Invitrogen

ATP6V1B1 Polyclonal Antibody

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ATP6V1B1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of V-ATPase B1 in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with V-ATPase B1 polyclonal antibody (Product # PA5-101951) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

ATP6V1B1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded V-ATPase B1 in human urothelial cancer tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with V-ATPase B1 polyclonal antibody (Product # PA5-101951) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

ATP6V1B1 Antibody in Western Blot (WB) — Western blot analysis of V-ATPase B1 in HepG2 cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with V-ATPase B1 polyclonal antibody (Product # PA5-101951).

Product Details

PA5-101951

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human ATP6V1B1(Accession P15313), corresponding to amino acid residues M396-C419. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2851383

Target Information

Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and a integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A-H) make up the V1 domain and five subunits (a, d, c, c' and c") make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. The V-ATPase V1 B subunit exists as two isoforms. In the inner ear, the V-ATPase B1 isoform functions in proton secretion and is required to maintain proper endolymph pH and normal auditory function. The gene encoding the human V-ATPase B1 isoform maps to chromosome 2cen-q13. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. The V-ATPase B2 isoform is expressed in kidney and is the only B isoform expressed in osteoclasts.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta 56/58 kDa, isoform 1; ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 1; ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1; ATPase, H+ transporting, V1 subunit B, isoform 1; Endomembrane proton pump 58 kDa subunit; H(+)-transporting two-sector ATPase, 58kD subunit; H+-ATPase beta 1 subunit; lysosomal 56/58kDa; V-ATPase B1; V-ATPase B1 subunit; V-ATPase subunit B 1; V-type proton ATPase subunit B, kidney isoform; vacuolar proton pump 3; Vacuolar proton pump subunit B 1; vacuolar proton pump, subunit 3

Gene Aliases: ATP6B1; ATP6V1B1; AW208839; D630003L15; D630030L16Rik; D630039P21Rik; RTA1B; VATB; VMA2; Vpp-3; VPP3

UniProt ID: (Human) P15313

Entrez Gene ID: (Human) 525, (Mouse) 110935

Molecular Function: ATP synthase