Proteintech

ATP6V1E1 Polyclonal Antibody

ATP6V1E1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ATP6V1E1 Antibody (15280-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using (Product # 15280-1-AP) (ATP6V1E1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATP6V1E1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15280-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ATP6V1E1 Fusion Protein Ag7399 (1-226 aa encoded by BC004443)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MALSDADVQ KQIKHMMAFI EQEANEKAEE IDAKAEEEFN IEKGRLVQTQ RLKIMEYYEK KEKQIEQQKK IQMSNLMNQA RLKVLRARDD LITDLLNEAK QRLSKVVKDT TRYQVLLDGL VLQGLYQLLE PRMIVRCRKQ DFPLVKAAVQ KAIPMYKIAT KNDVDVQIDQ ESYLPEDIAG GVEIYNGDRK IKVSNTLESR LDLIAQQMMP EVRGALFGAN ANRKFLD (1-226 aa encoded by BC004443)

Target Information

Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification.

Journal of cell science

Choezom D,Gross JC

Tue Mar 01 00:00:00 EST 2022

Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase assembly.

Nature communications

Ratto E,Chowdhury SR,Siefert NS,Schneider M,Wittmann M,Helm D,Palm W

Wed Aug 17 00:00:00 EDT 2022

Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy.

Microorganisms

Yuan L,Chu B,Chen S,Li Y,Liu N,Zhu Y,Zhou D

Tue Nov 16 00:00:00 EST 2021

Butyrate-mediated autophagy inhibition limits cytosolic Salmonella Infantis replication in the colon of pigs treated with a mixture of Lactobacillus and Bacillus.

Veterinary research

Chu B,Zhu Y,Su J,Xia B,Zou Y,Nie J,Zhang W,Wang J

Wed Aug 05 00:00:00 EDT 2020

iTRAQ-based quantitative proteomic analysis for identification of oligodendroglioma biomarkers related with loss of heterozygosity on chromosomal arm 1p.

Journal of proteomics

Yang LS,Xu XE,Liu XP,Jin H,Chen ZQ,Liu XH,Wang Y,Huang FP,Shi Q

Fri Dec 21 00:00:00 EST 2012

iTRAQ-based quantitative proteomic analysis for identification of oligodendroglioma biomarkers related with loss of heterozygosity on chromosomal arm 1p.

Journal of proteomics

Yang LS,Xu XE,Liu XP,Jin H,Chen ZQ,Liu XH,Wang Y,Huang FP,Shi Q

Fri Dec 21 00:00:00 EST 2012

iTRAQ-based quantitative proteomic analysis for identification of oligodendroglioma biomarkers related with loss of heterozygosity on chromosomal arm 1p.

Journal of proteomics

Yang LS,Xu XE,Liu XP,Jin H,Chen ZQ,Liu XH,Wang Y,Huang FP,Shi Q

Fri Dec 21 00:00:00 EST 2012

iTRAQ-based quantitative proteomic analysis for identification of oligodendroglioma biomarkers related with loss of heterozygosity on chromosomal arm 1p.

Journal of proteomics

Yang LS,Xu XE,Liu XP,Jin H,Chen ZQ,Liu XH,Wang Y,Huang FP,Shi Q

Fri Dec 21 00:00:00 EST 2012

Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification.

Proceedings of the National Academy of Sciences of the United States of America

Wong D,Bach H,Sun J,Hmama Z,Av-Gay Y

Tue Nov 29 00:00:00 EST 2011

Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification.

Proceedings of the National Academy of Sciences of the United States of America

Wong D,Bach H,Sun J,Hmama Z,Av-Gay Y

Tue Nov 29 00:00:00 EST 2011

Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification.

Proceedings of the National Academy of Sciences of the United States of America

Wong D,Bach H,Sun J,Hmama Z,Av-Gay Y

Tue Nov 29 00:00:00 EST 2011

Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification.

Proceedings of the National Academy of Sciences of the United States of America

Wong D,Bach H,Sun J,Hmama Z,Av-Gay Y

Tue Nov 29 00:00:00 EST 2011

Bioinformatics

Protein Aliases: ATPase, H+ transporting lysosomal (vacuolar proton pump), 32 kDa; ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E; ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1; ATPase, H+ transporting, V1 subunit E; H(+)-ATPase E-like protein; H(+)-transporting two-sector ATPase, 31kDa subunit; H+ ATPase subunit E; H+-transporting ATP synthase chain E, vacuolar; lysosomal 31kDa; p31; V-ATPase 31 kDa subunit; V-ATPase subunit E 1; V-ATPase, subunit E; V-type proton ATPase subunit E 1; vacuolar H(+)-ATPase, E subunit; vacuolar H+ ATPase E1; Vacuolar proton pump subunit E 1; VATPase, H+ transporting, lysosomal V1 subunit E1

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Gene Aliases: 2410029D23Rik; ATP6E; ATP6E2; ATP6V1E; ATP6V1E1; D6Ertd385e; E2; P31; Vma4

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UniProt ID: (Human) P36543, (Mouse) P50518, (Rat) Q6PCU2

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Entrez Gene ID: (Human) 529, (Mouse) 11973, (Rat) 297566

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Function(s)

protein binding hydrogen-exporting ATPase activity, phosphorylative mechanism proton-transporting ATPase activity, rotational mechanism ATPase binding hydrolase activity

Process(es)

transport ion transport ATP hydrolysis coupled proton transport proton transport metabolic process insulin receptor signaling pathway regulation of macroautophagy transferrin transport ion transmembrane transport phagosome acidification

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATP6V1E1 Polyclonal Antibody

ATP6V1E1 Antibody (15280-1-AP) in IHC (P)

Product Details

15280-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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