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Invitrogen
ATR Polyclonal Antibody
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FIGURE: 1 / 8
ATR Antibody (PA1-450) in WB
Western blot analysis of ATR was performed with 10 µg of HeLa cells transfected with Transfection Reagent alone (Lane 1), 100nM Non-Targeting control siRNA (Lane 2), or 100nM siRNA against ATR (Lane 3). Proteins were resolved using a NuPAGE® Novex 4-12% Bis-Tris Gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and a protein size ladder. Proteins were wet transferred to a Pierce Nitrocellulose Membrane (Product # 88025) OR Pierce PVDF Membrane (Product # 88518) and blocked with Pierce Starting Block T20 ... View More
Product Details
PA1-450
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Fusion protein containing residues 400-460 of the human ATR protein.
Conjugate
Form
Liquid
Concentration
4 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-450 detects ATR (Ataxia Telangiectasia Mutated (ATM) and Rad3-related protein) from human cells.
PA1-450 has been successfully used in Western blot immunofluoresence and immunoprecipitation procedures. By Western blot, this antibody detects an ~305 kDa protein representing ATR in lysate from UV irradiated K562 cells.
PA1-450 antigen is a fusion protein corresponding to amino acid residues 400-460 from human ATR.
Target Information
The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 2310008J16Rik; 2810405N18Rik; Ataxia telangiectasia and Rad3-related protein; atr; ATR serine/threonine kinase; EC 2.7.11.1; FRAP-related protein 1; FRAP-related protein-1; kinase ATR; MEC1, mitosis entry checkpoint 1, homolog; protein kinase ATR; Serine/threonine-protein kinase ATR; TEM8
Gene Aliases: ATR; FCTCS; FRP1; MEC1; SCKL; SCKL1
UniProt ID: (Human) Q13535
Entrez Gene ID: (Human) 545
DNA damage checkpoint
G2/M transition of mitotic cell cycle
telomere maintenance
nucleobase-containing compound metabolic process
DNA replication
DNA repair
double-strand break repair
chromatin remodeling
cellular response to DNA damage stimulus
nuclear envelope organization
negative regulation of DNA replication
response to xenobiotic stimulus
response to mechanical stimulus
replication fork processing
positive regulation of telomere maintenance via telomerase
cellular response to UV
interstrand cross-link repair
positive regulation of DNA damage response, signal transduction by p53 class mediator
mitotic G2/M transition checkpoint
response to arsenic-containing substance
nuclear envelope disassembly
protein localization to chromosome, telomeric region
cellular response to gamma radiation
regulation of cellular response to stress
replicative senescence
establishment of RNA localization to telomere
establishment of macromolecular complex localization to telomere
DNA strand resection involved in replication fork processing
regulation of cellular response to heat
positive regulation of telomerase catalytic core complex assembly
protein localization to site of double-strand break
regulation of double-strand break repair
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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