|Immunofluorescence (IF)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1,000|
|Western Blot (WB)||See 13 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion protein containing residues 400-460 of the human ATR protein.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-450 detects ATR (Ataxia Telangiectasia Mutated (ATM) and Rad3-related protein) from human cells.
PA1-450 has been successfully used in Western blot immunofluoresence and immunoprecipitation procedures. By Western blot, this antibody detects an ~305 kDa protein representing ATR in lysate from UV irradiated K562 cells.
PA1-450 antigen is a fusion protein corresponding to amino acid residues 400-460 from human ATR.
Ataxia Telangiectasia Mutated (ATM) and Rad3-related protein (ATR) is a phosphatidylinositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR is a member of the DNA-PK kinases closely related to ATM and DNA-PK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes. Several known components of the NuRD complex including histone deacetylase 1 (HDAC1), HDAC2, and CHD4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Ataxia telangiectasia and Rad3-related protein; Ataxia Telangiectasia Mutated (ATM) and Rad3-Related Protein; FRAP-related protein 1; FRAP-related protein-1; MEC1, mitosis entry checkpoint 1, homolog; Serine/threonine-protein kinase ATR
Gene Aliases: ATR; FCTCS; FRP1; MEC1; SCKL; SCKL1
UniProt ID: (Human) Q13535
Entrez Gene ID: (Human) 545
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