Search
Invitrogen
ATR Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
{{$ctrl.translations['antibody.pdp.skuNotes.additionalInformation']}}:
FIGURE: 1 / 7
ATR Antibody (PA5-85507) in ICC/IF
ATR Polyclonal Antibody detects ATR protein at nucleolus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATR protein stained by ATR Polyclonal Antibody (Product # PA5-85507) diluted at 1:5,000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-85507
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ATR. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Positive Control: 293T, A431, HeLa, HepG2, Raji, Raji NE, Raji nuclear extract.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 2310008J16Rik; 2810405N18Rik; ataxia telangiectasia and Rad3-related protein; atr; ATR serine/threonine kinase; EC 2.7.11.1; FRAP-related protein-1; kinase ATR; MEC1, mitosis entry checkpoint 1, homolog; protein kinase ATR; TEM8; unnamed protein product
Gene Aliases: FCTCS; FRP1; MEC1; SCKL; SCKL1
UniProt ID: (Human) Q13535
Entrez Gene ID: (Human) 545, (Mouse) 245000
DNA damage checkpoint
G2/M transition of mitotic cell cycle
telomere maintenance
DNA replication
DNA repair
double-strand break repair
chromatin remodeling
protein phosphorylation
cellular response to DNA damage stimulus
nuclear envelope organization
embryo implantation
negative regulation of DNA replication
response to mechanical stimulus
replication fork processing
positive regulation of telomere maintenance via telomerase
cellular response to UV
positive regulation of DNA damage response, signal transduction by p53 class mediator
mitotic G2/M transition checkpoint
nuclear envelope disassembly
chromosome organization
protein localization to chromosome, telomeric region
cellular response to gamma radiation
replicative senescence
establishment of RNA localization to telomere
DNA strand resection involved in replication fork processing
positive regulation of telomerase catalytic core complex assembly
protein localization to site of double-strand break
regulation of double-strand break repair
response to xenobiotic stimulus
interstrand cross-link repair
response to arsenic-containing substance
regulation of cellular response to stress
establishment of macromolecular complex localization to telomere
regulation of cellular response to heat
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support