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Invitrogen
ATRX Polyclonal Antibody
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FIGURE: 1 / 11
ATRX Antibody (PA5-21348) in ICC/IF
Immunocytochemistry analysis of ATRX in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Samples were incubated with ATRX polyclonal antibody (Product # PA5-21348) using a dilution of 1:1,000 (green). Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 μm.
Product Details
PA5-21348
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human ATRX. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.27 mg/mL
Amount
27 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, NIH3T3, PC-12, Rat-2.
Predicted reactivity: Mouse (97%), Chimpanzee (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATRX encodes a protein that contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. The mutations of this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha thalassemia/mental retardation syndrome (X-linked); alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae); alpha thalassemia/mental retardation syndrome X-linked homolog; ATP-dependent helicase ATRX; ATRX; helicase 2; helicase II; Heterochromatin protein 2; HP1 alpha-interacting protein; HP1-BP38 protein; pABP-2; RP5-875J14.1; Transcriptional regulator ATRX; unnamed protein product; X-linked helicase II; X-linked nuclear protein; XNP; Znf-HX
Gene Aliases: 4833408C14Rik; AI447451; ATR2; ATRX; DXHXS6677E; HP1-BP38; Hp1bp2; Hp1bp38; JMS; MRX52; MRXS3; RAD54; RAD54L; XH2; XNP; ZNF-HX
UniProt ID: (Human) P46100, (Mouse) Q61687
Entrez Gene ID: (Human) 546, (Rat) 246284, (Mouse) 22589
nucleotide binding
DNA binding
chromatin binding
helicase activity
protein binding
ATP binding
zinc ion binding
DNA translocase activity
hydrolase activity
ATPase activity
chromatin DNA binding
histone binding
metal ion binding
histone H3K9me2/3 reader activity
chromo shadow domain binding
methylated histone binding
DNA-binding transcription factor
gene-specific transcriptional regulator
meiotic spindle organization
DNA repair
chromatin organization
nucleosome assembly
chromatin remodeling
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
spermatogenesis
regulation of cell cycle process
positive regulation of nuclear cell cycle DNA replication
DNA damage response, signal transduction by p53 class mediator
forebrain development
replication fork processing
telomeric heterochromatin assembly
positive regulation of telomere maintenance
regulation of chromosome organization
post-embryonic forelimb morphogenesis
multicellular organism growth
positive regulation of transcription from RNA polymerase II promoter
Sertoli cell development
chromosome organization involved in meiotic cell cycle
protein localization to chromosome, telomeric region
seminiferous tubule development
cellular response to hydroxyurea
negative regulation of maintenance of mitotic sister chromatid cohesion, telomeric
DNA replication-independent nucleosome assembly
transcription, DNA-templated
biological_process
negative regulation of telomeric RNA transcription from RNA pol II promoter
chromatin modification
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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