Proteintech

AXL Polyclonal Antibody

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Antibody Testing Data (6)

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AXL Antibody (13196-1-AP) in WB

Human liver tissue were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Image Gallery

AXL Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug AXL antibody (13196-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

AXL Antibody in Immunoprecipitation (IP) — IP result of anti-AXL (IP:13196-1-AP, 5ug; Detection:13196-1-AP 1:1000) with HepG2 cells lysate 9000ug.

Product Details

13196-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

AXL Fusion Protein Ag3898 (545-894 aa encoded by BC032229)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody detects a single band at 140 kDa, which is considered to represent the auto-phosphorylated form of endogenous Axl.

Immunogen sequence: GEFGAVMEG QLNQDDSILK VAVKTMKIAI CTRSELEDFL SEAVCMKEFD HPNVMRLIGV CFQGSERESF PAPVVILPFM KHGDLHSFLL YSRLGDQPVY LPTQMLVKFM ADIASGMEYL STKRFIHRDL AARNCMLNEN MSVCVADFGL SKKIYNGDYY RQGRIAKMPV KWIAIESLAD RVYTSKSDVW SFGVTMWEIA TRGQTPYPGV ENSEIYDYLR RGNRLKQPAD CLDGLYALMS RCWELNPQDR PSFTELREDL ENTLKALPPA QEPDEILYVN MDEGGGYPEP PGAAGGADPP TQPDPKDSCS CLTAAEVHPA GRYVLCPSTT PSPAQPADRG SPAAPGQEDG A (545-894 aa encoded by BC032229)

Target Information

AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma. The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Axl promotes intracranial aneurysm rupture by regulating macrophage polarization toward M1 via STAT1/HIF-1α.

Frontiers in immunology

Han Y,Li G,Zhang Z,Zhang X,Zhao B,Yang H

Fri May 26 00:00:00 EDT 2023

Axl promotes intracranial aneurysm rupture by regulating macrophage polarization toward M1 via STAT1/HIF-1α.

Frontiers in immunology

Han Y,Li G,Zhang Z,Zhang X,Zhao B,Yang H

Fri May 26 00:00:00 EDT 2023

NAT10-mediated AXL mRNA N4-acetylcytidine modification promotes pancreatic carcinoma progression.

Experimental cell research

Zong G,Wang X,Guo X,Zhao Q,Wang C,Shen S,Xiao W,Yang Q,Jiang W,Shen J,Wan R

Sat Jul 15 00:00:00 EDT 2023

SUFU suppresses ferroptosis sensitivity in breast cancer cells via Hippo/YAP pathway.

iScience

Fang K,Du S,Shen D,Xiong Z,Jiang K,Liang D,Wang J,Xu H,Hu L,Zhai X,Jiang Y,Xia Z,Xie C,Jin D,Cheng W,Meng S,Wang Y

Fri Jul 15 00:00:00 EDT 2022

Combination of sunitinib and ¹⁷⁷Lu-labeled antibody cG250 targeted radioimmunotherapy: A promising new therapeutic strategy for patients with advanced renal cell cancer.

Neoplasia (New York, N.Y.)

Oosterwijk-Wakka JC,de Weijert MCA,Franssen GM,Kolev DR,de Haan TAFJ,Boerman OC,Mulders PFA,Oosterwijk E

Sat Oct 01 00:00:00 EDT 2022

Combination of sunitinib and ¹⁷⁷Lu-labeled antibody cG250 targeted radioimmunotherapy: A promising new therapeutic strategy for patients with advanced renal cell cancer.

Neoplasia (New York, N.Y.)

Oosterwijk-Wakka JC,de Weijert MCA,Franssen GM,Kolev DR,de Haan TAFJ,Boerman OC,Mulders PFA,Oosterwijk E

Sat Oct 01 00:00:00 EDT 2022

Combination of sunitinib and ¹⁷⁷Lu-labeled antibody cG250 targeted radioimmunotherapy: A promising new therapeutic strategy for patients with advanced renal cell cancer.

Neoplasia (New York, N.Y.)

Oosterwijk-Wakka JC,de Weijert MCA,Franssen GM,Kolev DR,de Haan TAFJ,Boerman OC,Mulders PFA,Oosterwijk E

Sat Oct 01 00:00:00 EDT 2022

Combination of sunitinib and ¹⁷⁷Lu-labeled antibody cG250 targeted radioimmunotherapy: A promising new therapeutic strategy for patients with advanced renal cell cancer.

Neoplasia (New York, N.Y.)

Oosterwijk-Wakka JC,de Weijert MCA,Franssen GM,Kolev DR,de Haan TAFJ,Boerman OC,Mulders PFA,Oosterwijk E

Sat Oct 01 00:00:00 EDT 2022

Identification of an AXL kinase inhibitor in triple-negative breast cancer by structure-based virtual screening and bioactivity test.

Chemical biology & drug design

Li P,Niu Y,Li S,Zu X,Xiao M,Yin L,Feng J,He J,Shen Y

Tue Feb 01 00:00:00 EST 2022

Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop.

Cell death & disease

Shen Y,Zhu Q,Xiao M,Yin L,Feng W,Feng J,He J,Li P,Chen X,Ding W,Zhong J,Zeng Z,Xie Z,Liu J,Zu X

Tue Aug 30 00:00:00 EDT 2022

Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop.

Cell death & disease

Shen Y,Zhu Q,Xiao M,Yin L,Feng W,Feng J,He J,Li P,Chen X,Ding W,Zhong J,Zeng Z,Xie Z,Liu J,Zu X

Tue Aug 30 00:00:00 EDT 2022

Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop.

Cell death & disease

Shen Y,Zhu Q,Xiao M,Yin L,Feng W,Feng J,He J,Li P,Chen X,Ding W,Zhong J,Zeng Z,Xie Z,Liu J,Zu X

Tue Aug 30 00:00:00 EDT 2022

Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop.

Cell death & disease

Shen Y,Zhu Q,Xiao M,Yin L,Feng W,Feng J,He J,Li P,Chen X,Ding W,Zhong J,Zeng Z,Xie Z,Liu J,Zu X

Tue Aug 30 00:00:00 EDT 2022

Activated AXL Protects Against Hepatic Ischemia-reperfusion Injury by Upregulating SOCS-1 Expression.

Transplantation

Wang Z,Liu D,Yan Q,Liu F,Zhan M,Qi S,Fang Q,Yao L,Wang W,Zhang R,Du J,Chen L

Fri Jul 01 00:00:00 EDT 2022

Activated AXL Protects Against Hepatic Ischemia-reperfusion Injury by Upregulating SOCS-1 Expression.

Transplantation

Wang Z,Liu D,Yan Q,Liu F,Zhan M,Qi S,Fang Q,Yao L,Wang W,Zhang R,Du J,Chen L

Fri Jul 01 00:00:00 EDT 2022

Activated AXL Protects Against Hepatic Ischemia-reperfusion Injury by Upregulating SOCS-1 Expression.

Transplantation

Wang Z,Liu D,Yan Q,Liu F,Zhan M,Qi S,Fang Q,Yao L,Wang W,Zhang R,Du J,Chen L

Fri Jul 01 00:00:00 EDT 2022

Activated AXL Protects Against Hepatic Ischemia-reperfusion Injury by Upregulating SOCS-1 Expression.

Transplantation

Wang Z,Liu D,Yan Q,Liu F,Zhan M,Qi S,Fang Q,Yao L,Wang W,Zhang R,Du J,Chen L

Fri Jul 01 00:00:00 EDT 2022

GPI-anchored ligand-BioID2-tagging system identifies Galectin-1 mediating Zika virus entry.

iScience

Gao SS,Shi R,Sun J,Tang Y,Zheng Z,Li JF,Li H,Zhang J,Leng Q,Xu J,Chen X,Zhao J,Sy MS,Feng L,Li C

Thu Dec 22 00:00:00 EST 2022

Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.

International journal of molecular sciences

De Tomi E,Campagnari R,Orlandi E,Cardile A,Zanrè V,Menegazzi M,Gomez-Lira M,Gotte G

Mon Jan 31 00:00:00 EST 2022

Gas6 Promotes Microglia Efferocytosis and Suppresses Inflammation Through Activating Axl/Rac1 Signaling in Subarachnoid Hemorrhage Mice.

Translational stroke research

Tang J,Jin Y,Jia F,Lv T,Manaenko A,Zhang LF,Zhang Z,Qi X,Xue Y,Zhao B,Zhang X,Zhang JH,Lu J,Hu Q

Thu Nov 03 00:00:00 EDT 2022

Gas6 Promotes Microglia Efferocytosis and Suppresses Inflammation Through Activating Axl/Rac1 Signaling in Subarachnoid Hemorrhage Mice.

Translational stroke research

Tang J,Jin Y,Jia F,Lv T,Manaenko A,Zhang LF,Zhang Z,Qi X,Xue Y,Zhao B,Zhang X,Zhang JH,Lu J,Hu Q

Thu Nov 03 00:00:00 EDT 2022

Embryonic-Derived Myb^- Macrophages Enhance Bacterial Clearance and Improve Survival in Rat Sepsis.

International journal of molecular sciences

Jerkic M,Litvack ML,Gagnon S,Otulakowski G,Zhang H,Rotstein O,Kavanagh BP,Post M,Laffey JG

Sat Mar 20 00:00:00 EDT 2021

Non-volatile acylphloroglucinol components from Eucalyptus robusta inhibit Zika virus by impairing RdRp activity of NS5.

Bioorganic chemistry

Yao ZW,Liu H,Zhou R,Feng MY,Wang F,Qin XJ,Chen XX,Zheng CB,Luo RH,Yang LM,Cen S,Xiong SD,Liu HY,Zheng YT

Mon Nov 01 00:00:00 EDT 2021

Cell membrane skeletal protein 4.1R participates in entry of Zika virus into cells.

Virus research

Su S,Liu X,Tian RR,Qiao KX,Zheng CB,Gao WC,Yang LM,Kang QZ,Zheng YT

Wed Dec 01 00:00:00 EST 2021

Ginsenoside Rb1 attenuates age-associated vascular impairment by modulating the Gas6 pathway.

Pharmaceutical biology

Ke S,Wu L,Wang M,Liu D,Shi G,Zhu J,Qian X

Wed Dec 01 00:00:00 EST 2021

Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.

Cancer gene therapy

Dong M,Xiao Q,Hu J,Cheng F,Zhang P,Zong W,Tang Q,Li X,Mao F,He Y,Yu X,Wan F,Lei T,Guo D,Wang B

Tue Dec 01 00:00:00 EST 2020

Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.

Cancer gene therapy

Dong M,Xiao Q,Hu J,Cheng F,Zhang P,Zong W,Tang Q,Li X,Mao F,He Y,Yu X,Wan F,Lei T,Guo D,Wang B

Tue Dec 01 00:00:00 EST 2020

Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.

Cancer gene therapy

Dong M,Xiao Q,Hu J,Cheng F,Zhang P,Zong W,Tang Q,Li X,Mao F,He Y,Yu X,Wan F,Lei T,Guo D,Wang B

Tue Dec 01 00:00:00 EST 2020

Therapeutic activity of DCC-2036, a novel tyrosine kinase inhibitor, against triple-negative breast cancer patient-derived xenografts by targeting AXL/MET.

International journal of cancer

Shen Y,Zhang W,Liu J,He J,Cao R,Chen X,Peng X,Xu H,Zhao Q,Zhong J,Ding W,Lei X,Jiang Y,Zu X

Fri Feb 01 00:00:00 EST 2019

Therapeutic activity of DCC-2036, a novel tyrosine kinase inhibitor, against triple-negative breast cancer patient-derived xenografts by targeting AXL/MET.

International journal of cancer

Shen Y,Zhang W,Liu J,He J,Cao R,Chen X,Peng X,Xu H,Zhao Q,Zhong J,Ding W,Lei X,Jiang Y,Zu X

Fri Feb 01 00:00:00 EST 2019

Vitamin K2 inhibits rat vascular smooth muscle cell calcification by restoring the Gas6/Axl/Akt anti-apoptotic pathway.

Molecular and cellular biochemistry

Qiu C,Zheng H,Tao H,Yu W,Jiang X,Li A,Jin H,Lv A,Li H

Fri Sep 01 00:00:00 EDT 2017

Vitamin K2 inhibits rat vascular smooth muscle cell calcification by restoring the Gas6/Axl/Akt anti-apoptotic pathway.

Molecular and cellular biochemistry

Qiu C,Zheng H,Tao H,Yu W,Jiang X,Li A,Jin H,Lv A,Li H

Fri Sep 01 00:00:00 EDT 2017

Vitamin K2 regression aortic calcification induced by warfarin via Gas6/Axl survival pathway in rats.

European journal of pharmacology

Jiang X,Tao H,Qiu C,Ma X,Li S,Guo X,Lv A,Li H

Mon Sep 05 00:00:00 EDT 2016

Vitamin K2 regression aortic calcification induced by warfarin via Gas6/Axl survival pathway in rats.

European journal of pharmacology

Jiang X,Tao H,Qiu C,Ma X,Li S,Guo X,Lv A,Li H

Mon Sep 05 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Adhesion-related kinase; AXL oncogene; AXL transforming sequence/gene; AZF1; oncogene AXL; sAXL; soluble AXL; Tyrosine-protein kinase receptor UFO; ufo oncogene homolog

Gene Aliases: AI323647; ARK; AXL; JTK11; Tyro7; UFO

UniProt ID: (Human) P30530, (Mouse) Q00993

Entrez Gene ID: (Human) 558, (Mouse) 26362

Molecular Function: transmembrane signal receptor