Invitrogen

Activin A Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (22) Activin A antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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Activin A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Inhibin beta-A (Product # PA5-21939) antibody at 1:200 dilution.

Activin A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded CLEAR CELL OVCA xenograft, using Inhibin beta A (Product # PA5-21939) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Activin A Antibody in Western Blot (WB) — Western Blot using Activin A Polyclonal Antibody (Product # PA5-21939). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Activin A Polyclonal Antibody (Product # PA5-21939) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Activin A Antibody — Figure 5 In vitro models of DKD-related injury. HK-2 studies following incubation with high glucose (HG, 25 mmol/L), transforming growth factor-beta1 (TGF-beta1; 5 ng/mL) and albumin (ALB, 5 mg/mL). Time-dependent effect of HG+TGF-beta1 on the mRNA/gene expression of activin A, monocyte chemoattractant protein-1 (MCP-1; an inflammatory marker), type I collagen (mesenchymal marker) and E-cadherin (epithelial marker) in injured HK-2 cells (A) Immunofluorescent staining of HK-2 cells injured by HG+TGF-beta1 at 12 hours (h) compared with baseline (B) Time-dependent effect for activin A and p16 expression in injured HK-2 cells (C) Protein levels of activin A in the supernatant of HK-2 treated with HG+TGF-beta1 (D) and combinations of HG with or without ALB (E) at 24 hours. Values are mean+-SEM. a P<0.05 compared with untreated HK-2 groups; b p<0.05 compared with the previous group; c p<0.05 compared with all prior groups. Scale bars: 20 um. {TM}

Product Details

PA5-21939

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to a region within amino acids 362 and 426 of Inhibin beta A View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.66 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11153440

Product Specific Information

Recommended positive controls: A549, H1299, HCT-116.

Predicted reactivity: Mouse (100%), Rat (100%), Cat (100%), Pig (100%), Rabbit (100%), Chicken (100%), Sheep (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Activin A mutations are associated with fibrodysplasia ossificans progressive.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Senescence marker activin A is increased in human diabetic kidney disease: association with kidney function and potential implications for therapy.

BMJ open diabetes research & care

Bian X,Griffin TP,Zhu X,Islam MN,Conley SM,Eirin A,Tang H,O'Shea PM,Palmer AK,McCoy RG,Herrmann SM,Mehta RA,Woollard JR,Rule AD,Kirkland JL,Tchkonia T,Textor SC,Griffin MD,Lerman LO,Hickson LJ

Published figure using Activin A polyclonal antibody (Product # PA5-21939) in Immunocytochemistry

Mon Aug 31 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: Activin beta-A chain; EDF; erythroid differentiation factor; Erythroid differentiation protein; follicle-stimulating hormone-releasing protein; FSH-releasing protein; Inhibin beta A chain; inhibin, beta A (activin A, activin AB alpha polypeptide); Inhibin, beta-1

Gene Aliases: EDF; FRP; INHBA

UniProt ID: (Human) P08476

Entrez Gene ID: (Human) 3624

Molecular Function: growth factor

Function(s)

Process(es)

cytokine activity transforming growth factor beta receptor binding hormone activity protein binding growth factor activity peptide hormone binding inhibin binding identical protein binding protein heterodimerization activity type II activin receptor binding

Activin A Polyclonal Antibody

Product Details

PA5-21939

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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