|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 15 amino acid peptide located near human Adiponectin carboxy-terminus.|
|Contains||0.02% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay dependent|
|Immunocytochemistry (ICC)||10 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||10 ug/ml|
|Western Blot (WB)||1 - 2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. In Western blot, this antibody detects a band at ~26.4kDa in human HL60 cell lysates. A suggested positive control for PA1-84881 is rat brain.
Adiponectin, also known as Acrp30, is a major adipokine secreted into the bloodstream from adipose tissue to modulate metabolism, including glucose regulation and fatty acid catabolism. Unlike most other adipokines, Adiponectin is secreted exclusively by differentiating adipocytes at reduced levels during obesity. The serum level of Adiponectin is inversely correlated with BMI (body mass index) of an individual, and has an anti-inflammatory action, playing an important role in type II diabetes (insulin sensitivity) and atherosclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.