Western blot analysis of Adiponectin Receptor 1 was performed by loading 25 ug of rat skeletal muscle (lane 1), human skeletal muscle (lane 2) and HepG2 (lane 3) lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with an Adiponectin Receptor 1 polyclonal antibody (Product # PA1-059) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~42kDa.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues R(20) E A D T V E L A E L G P L L E E K(37) of human Adiponectin Receptor 1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-059 detects human and rat adiponectin receptor 1.
PA1-059 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~42 kDa protein corresponding to human adiponectin receptor 1 from transfected hepatocytes.
The PA1-059 immunogen is a synthetic peptide corresponding to residues R(20) E A D T V E L A E L G P L L E E K(37) of human Adiponectin Receptor 1.
Adiponectin (also known as 30-kDa adipocyte complement-related protein; Acrp30) is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Levels of adiponectin in the blood are decreased under conditions of obesity, insulin resistance and type 2 diabetes. Administration of adiponectin causes glucose-lowering effects and ameliorates insulin resistance in mice. Conversely, adiponectin-deficient mice exhibit insulin resistance and diabetes. This insulin-sensitizing effect of adiponectin seems to be mediated by an increase in fatty-acid oxidation through activation of AMP kinase and PPAR- . Here we report the cloning of complementary DNAs encoding adiponectin receptors 1 and 2 (AdipoR1 and AdipoR2) by expression cloning. AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. These two adiponectin receptors are predicted to contain seven transmembrane domains, but are structurally and functionally distinct from G-protein-coupled receptors. Expression of AdipoR1/R2 or suppression of AdipoR1/R2 expression by small-interfering RNA supports our conclusion that they serve as receptors for globular and full-length adiponectin, and that they mediate increased AMP kinase and PPAR- ligand activities, as well as fatty-acid oxidation and glucose uptake by adiponectin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Increase of adiponectin receptor gene expression by physical exercise in soleus muscle of obese Zucker rats.
PA1-059 was used in western blot to investigate the influence of physical exercise on AdipoR1 level in obese rats
|Chang SP,Chen YH,Chang WC,Liu IM,Cheng JT||European journal of applied physiology (97:189)||2006|