Immunofluorescent analysis of Adiponectin Receptor 2 was performed using 70% confluent 3T3-L1 cells differentiated with StemPro Adipogenesis Supplement (A10065-01) for 5 days. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Adiponectin Receptor 2 Rabbit Polyclonal Antibody (PA1-1071) at 2 ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues D M F A T P Q Y R G V R of mouse Adiponectin Receptor 2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/mL|
|Immunofluorescence (IF)||2 µg/mL|
|Western Blot (WB)||1:50-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
PA1-1071 detects Adiponectin Receptor 2 from mouse and rat samples.
PA1-1071 has been successfully used in Western blot applications. By Western blot, this antibody detects a single ~43 kDa band representing Adiponectin Receptor 2 from mouse liver.
The PA1-1071 immunogen is a synthetic peptide corresponding to residues D M F A T P Q Y R G V R of mouse Adiponectin Receptor 2.
Adiponectin (also known as 30-kDa adipocyte complement-related protein; Acrp30) is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Levels of adiponectin in the blood are decreased under conditions of obesity, insulin resistance and type 2 diabetes. Administration of adiponectin causes glucose-lowering effects and ameliorates insulin resistance in mice. Conversely, adiponectin-deficient mice exhibit insulin resistance and diabetes. This insulin-sensitizing effect of adiponectin seems to be mediated by an increase in fatty-acid oxidation through activation of AMP kinase and PPAR- . Here we report the cloning of complementary DNAs encoding adiponectin receptors 1 and 2 (AdipoR1 and AdipoR2) by expression cloning. AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. These two adiponectin receptors are predicted to contain seven transmembrane domains, but are structurally and functionally distinct from G-protein-coupled receptors. Expression of AdipoR1/R2 or suppression of AdipoR1/R2 expression by small-interfering RNA supports our conclusion that they serve as receptors for globular and full-length adiponectin, and that they mediate increased AMP kinase and PPAR- ligand activities, as well as fatty-acid oxidation and glucose uptake by adiponectin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A peptide-modified chitosan-collagen hydrogel for cardiac cell culture and delivery.
PA1-1071 was used in western blot to evaluate a peptide-modified chitosan-collagen hydrogel for cardiac cell culture and delivery
|Reis LA,Chiu LL,Liang Y,Hyunh K,Momen A,Radisic M||Acta biomaterialia (8:1022)||2012|
Regulation of adiponectin secretion by soy isoflavones has implication for endocrine function of the testis.
PA1-1071 was used in western blot to investigate the effect of soy isoflavones on adiponectin and on endocrine function of the testis
|Pfaehler A,Nanjappa MK,Coleman ES,Mansour M,Wanders D,Plaisance EP,Judd RL,Akingbemi BT||Toxicology letters (209:78)||2012|