PA1-1746 detects human, rat, mouse, bovine, canine, and porcine aggrecan neo G1.
PA1-1746 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~64 kDa protein representing the neoepitope sequence (NITEGE) generated by aggrecanase-mediated cleavage at Glu373-Ala374 of aggrecan core proteins.
The PA1-1746 immunogen is a synthetic peptide corresponding to residues residues C G G N(387) I T E G E(392) of human aggrecan.
Aggrecan is a member of large, aggregating proteoglycans (also including, versican, brevican and neurocan) found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMP's) and aggrecanase (ADAMTS). Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis.
The C-terminal NITEGE fragment is one of the G1 fragments produced when aggrecan is cleaved with aggrecanase. It is the most abundant fragment found in normal human synovial fluid and articular cartilage, and is the major product produced by aggrecanase cleavage. The NITEGE fragment has also been shown to accumulate rapidly after an acute cartilage injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AGC1; AGCAN; aggrecan 1; aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122); Aggrecan core protein; Aggrecan core protein 2; aggrecan epidermal growth factor-like domain-1; aggrecan, structural proteoglycan of cartilage; cartilage aggregating proteoglycan; Cartilage-specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; Chondroitin sulfate proteoglycan core protein 1; CSPCP; CSPG1; CSPGCP; large aggregating proteoglycan; large aggregating proteoglycan, antigen; MSK16; SEDK
Gene Aliases: ACAN; Agc; AGC1; AGCAN; b2b183Clo; BOS_20116; cmd; CSPCP; CSPG1; CSPGCP; MSK16; SEDK