Invitrogen

Agrin Polyclonal Antibody

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Agrin Polyclonal Antibody

Product Details

PA5-109369

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 30-350 of human AGRN (NP_940978.2).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.67 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2854780

Product Specific Information

Immunogen sequence: TCPERALERR EEEANVVLTG TVEEILNVDP VQHTYSCKVR VWRYLKGKDL VARESLLDGG NKVVISGFGD PLICDNQVST GDTRIFFVNP APPYLWPAHK NELMLNSSLM RITLRNLEEV EFCVEDKPGT HFTPVPPTPP DACRGMLCGF GAVCEPNAEG PGRASCVCKK SPCPSVVAPV CGSDASTYSN ECELQRAQCS QQRRIRLLSR GPCGSRDPCS NVTCSFGSTC ARSADGLTAS CLCPATCRGA PEGTVCGSDG ADYPGECQLL RRACARQENV FKKFDGPCDP CQGALPDPSR SCRVNPRTRR PEMLLRPESC P

Target Information

Agrin is a molecule that resides in the basal lamina of muscle cells and directs key events in post synaptic differentiation. Most notably, Agrin is responsible for the clustering of acetylcholine receptors (AChRs) on the cell surface and their localization to the neuromuscular junction. Several Agrin variants have been identified which arise from alternative mRNA splicings. Agrin splice forms having inserts at two sites in the carboxy terminus designated "y" and "z" display a high affinity for AChRs, while splice forms lacking these inserts associate with AChRs weakly. Muscle alpha-dystroglycan has been postulated to be the receptor for the clustering activity of agrin; however, this is a point of contention. Tyrosine phosphorylation has been implicated as a required early step in AChR aggregation. Interestingly, a unique receptor tyrosine kinase, designated MuSK, has been discovered that interacts with Agrin and is specifically localized to developing muscle.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Agrin; agrin proteoglycan; RP23-139J18.2

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Gene Aliases: AGR; AGRIN; AGRN; CMS8; CMSPPD; nmf380

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UniProt ID: (Human) Q5SVA1, (Rat) P25304

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Entrez Gene ID: (Human) 375790, (Mouse) 11603, (Rat) 25592

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Function(s)
dystroglycan binding structural constituent of cytoskeleton calcium ion binding protein binding sialic acid binding chondroitin sulfate binding laminin binding heparan sulfate proteoglycan binding acetylcholine receptor regulator activity BMP binding ATPase inhibitor activity ion channel binding transforming growth factor beta binding protease inhibitor
Process(es)
retinoid metabolic process glycosaminoglycan biosynthetic process glycosaminoglycan catabolic process cytoskeleton organization signal transduction G-protein coupled acetylcholine receptor signaling pathway extracellular matrix organization glycosaminoglycan metabolic process receptor clustering positive regulation of GTPase activity clustering of voltage-gated sodium channels positive regulation of synaptic growth at neuromuscular junction positive regulation of transcription from RNA polymerase II promoter synapse organization positive regulation of filopodium assembly regulation of protein phosphorylation positive regulation of protein phosphorylation plasma membrane organization chemical synaptic transmission multicellular organism development synapse assembly neuromuscular junction development regulation of synaptic growth at neuromuscular junction regulation of receptor activity cell differentiation positive regulation of protein binding regulation of GTPase activity positive regulation of neuron apoptotic process neurotransmitter receptor metabolic process protein heterotetramerization regulation of cardiac muscle contraction positive regulation of protein tyrosine kinase activity regulation of microtubule cytoskeleton organization skeletal muscle acetylcholine-gated channel clustering regulation of cardiac muscle cell membrane potential regulation of axon guidance negative regulation of sodium ion export from cell negative regulation of sodium:potassium-exchanging ATPase activity positive regulation of protein geranylgeranylation
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