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Invitrogen
Alix Polyclonal Antibody
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FIGURE: 1 / 1
Alix Antibody (PA5-95321) in WB
Oncology letters 2024 -
Figure 1. Identification of plasma EVs. (A and B) Observation of EVs by transmission electron microscopy. (A) Scale bar, 500 nm. (B) Scale bar, 100 nm. (C) Nanoparticle tracking analysis. Left, sample EV particle size distribution map; right, display of particles in the nanoparticle tracking analysis detection window. Maximum area, 1,000 nm; minimum area, 5 nm; minimum brightness, 20 nm. (D) Qualitative det... View More
Product Details
PA5-95321
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human ALIX recombinant protein (Position: A2-D330).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Human ALIX shares 96.7% and 95.2% amino acid (aa) sequence identity with mouse and rat ALIX, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human HepG2 whole cell, human PANC-1 whole cell, rat liver tissue, rat RH35 whole cell, mouse liver tissue, mouse NIH/3T3 whole cell. IHC: rat liver tissue, huamn lung tissue, huamn oesophagus squama tissue, huamn placenta tissue, mouse intestine tissue. ICC/IF: T-47D cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Apoptotic linked-gene-product 2 (ALG-2) interacting protein X (ALIX) is a conserved adaptor protein which is ubiquitously expressed. Alix was originally reported to play a role in apoptosis but has recently been shown to be involved in other cellular mechanisms including endosomal sorting, endocytosis, viral budding and actin cytoskeleton assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ALG-2 interacting protein 1; ALG-2 interacting protein X; ALG-2-interacting protein 1; ALG-2-interacting protein X; Alg2-interacting protein 1; Alg2-interacting protein X; apoptosis-linked gene 2-interacting protein X; dopamine receptor interacting protein 4; E2F1-inducible protein; Eig2; Hp95; MGC17003; PDCD6-interacting protein; Programmed cell death 6-interacting protein; similar to XP95; possible role in signal transduction and cell growth/death; unnamed protein product
Gene Aliases: AI480591; AIP1; ALIX; AW544830; C76364; DRIP4; Eig2; HP95; KIAA1375; MCPH29; mKIAA1375; PDCD6IP; RGD1561176
UniProt ID: (Human) Q8WUM4, (Rat) Q9QZA2, (Mouse) Q9WU78
Entrez Gene ID: (Human) 10015, (Rat) 501083, (Mouse) 18571
mitotic cytokinesis
actomyosin contractile ring assembly
apoptotic process
nucleus organization
mitotic metaphase plate congression
regulation of centrosome duplication
protein transport
macroautophagy
multivesicular body assembly
viral budding via host ESCRT complex
maintenance of epithelial cell apical/basal polarity
viral budding
protein homooligomerization
cell division
midbody abscission
bicellular tight junction assembly
regulation of membrane permeability
ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
extracellular exosome biogenesis
regulation of mitotic spindle assembly
positive regulation of exosomal secretion
regulation of extracellular exosome assembly
positive regulation of extracellular exosome assembly
cell separation after cytokinesis
transport
cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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