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Invitrogen
Alpha A Crystallin Polyclonal Antibody
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Product Details
PA1-009
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Rat
Published species
Chicken,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified alpha-A Crystallin from bovine lens.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 30mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-009 detects alpha-A crystallin from bovine and rat samples. This antibody does not detect the unphosphorylated form of the protein.
PA1-009 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~20 kDa protein representing alpha-A crystallin from bovine lens samples. Immunohistochemical staining of alpha-A crystallin in rat thymus samples results in staining of epithelial reticular cells.
The PA1-009 immunogen is purified alpha-A Crystallin from bovine lens.
Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.
Target Information
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Acry 1; alpha-A-crystallin; Alpha-crystallin A chain; CRYA 1; Crystallin Alpha 1; Crystallin, alpha polypeptide A; HSPB 4
Gene Aliases: Acry-1; CRYA1; CRYAA
UniProt ID: (Bovine) P02470, (Rat) P24623
Entrez Gene ID: (Bovine) 281718, (Rat) 24273
response to hypoxia
positive regulation of protein phosphorylation
mitochondrion organization
actin filament organization
microtubule-based process
tubulin complex assembly
negative regulation of gene expression
positive regulation of cell growth
protein refolding
response to hydrogen peroxide
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
embryonic camera-type eye morphogenesis
apoptotic process involved in morphogenesis
response to UV-A
lens fiber cell morphogenesis
lens development in camera-type eye
lens morphogenesis in camera-type eye
protein folding
apoptotic process
visual perception
response to lead ion
negative regulation of intracellular transport
response to drug
camera-type eye development
negative regulation of apoptotic process
protein homooligomerization
response to glucocorticoid
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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