Proteintech

Amphiphysin Polyclonal Antibody

View all (10) Amphiphysin antibodies

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Questions & Answers

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Amphiphysin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer using 13379-1-AP (Amphiphysin antibody) at dilution of 1:100 (under 40x lens).

Amphiphysin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer using 13379-1-AP (Amphiphysin antibody) at dilution of 1:100 (under 10x lens).

Amphiphysin Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 13379-1-AP (Amphiphysin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Amphiphysin Antibody in Western Blot (WB) — Mouse lung tissue were subjected to SDS PAGE followed by western blot with 13379-1-AP (Amphiphysin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Amphiphysin Antibody in Immunoprecipitation (IP) — IP result of anti-Amphiphysin (IP:13379-1-AP, 4ug; Detection:13379-1-AP 1:1500) with mouse brain tissue lysate 4000ug.

Product Details

13379-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 3 publications 3 publications
Immunohistochemistry (IHC)	-	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	-	View 5 publications 5 publications
Flow Cytometry (Flow)	Assay-dependent	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Amphiphysin Fusion Protein Ag4204 (346-695 aa encoded by BC034376) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.31 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: KKEETLLDL DFDPFKPEVT PAGSAGVTHS PMSQTLPWDL WTTSTDLVQP ASGGSFNGFT QPQDTSLFTM QTDQSMICNL AESEQAPPTE PKAEEPLAAV TPAVGLDLGM DTRAEEPVEE AVIIPGADAD AAVGTLVSAA EGAPGEEAEA ETATVPAGEG VSLEEAKIGT ETTEGAESAQ PEAEELEATV PQEKVIPSVV IEPASNHEEE GENEITIGAE PKETTEDAAP PGPTSETPEL ATEQKPIQDP QPTPSAPAMG AADQLASARE ASQELPPGFL YKVETLHDFE AANSDELTLQ RGDVVLVVPS DSEADQDAGW LVGVKESDWL QYRDLATYKG LFPENFTRRL D (346-695 aa encoded by BC034376)

Target Information

Amphiphysins, members of the BAR (Bin-Amphiphysin-Rvsp) protein super family, play a key role in clathrin-mediated endocytosis of synaptic vesicles (SVs). All members of the BAR family share a highly conserved N-terminal BAR domain and a C-terminal Src homology (SH3) domain. Two isoforms of amphiphysin have been identified and act in concert as a heterodimer. Amphiphysins interact via its carboxy-terminal SH3 domain with dynamin, synaptojanin and clathrin. These complexes are implicated in synaptic vesicle recycling at nerve terminal. Amphiphysins were also identified as the dominant autoantigen in paraneoplastic Stiff-Man Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

AMPH-1 is critical for breast cancer progression.

Journal of Cancer

Chen Y,Liu J,Li L,Xia H,Lin Z,Zhong T

Tue Sep 29 00:00:00 EDT 2020

AMPH-1 is critical for breast cancer progression.

Journal of Cancer

Chen Y,Liu J,Li L,Xia H,Lin Z,Zhong T

Tue Sep 29 00:00:00 EDT 2020

AMPH-1 is a tumor suppressor of lung cancer by inhibiting Ras-Raf-MEK-ERK signal pathway.

Lasers in medical science

Yang H,Wan Z,Huang C,Yin H,Song D

Mon Apr 01 00:00:00 EDT 2019

AMPH-1 is a tumor suppressor of lung cancer by inhibiting Ras-Raf-MEK-ERK signal pathway.

Lasers in medical science

Yang H,Wan Z,Huang C,Yin H,Song D

Mon Apr 01 00:00:00 EDT 2019

AMPH-1 is a tumor suppressor of lung cancer by inhibiting Ras-Raf-MEK-ERK signal pathway.

Lasers in medical science

Yang H,Wan Z,Huang C,Yin H,Song D

Mon Apr 01 00:00:00 EDT 2019

Conditional ablation of the prorenin receptor in nephron progenitor cells results in developmental programming of hypertension.

Physiological reports

Song R,Kidd L,Janssen A,Yosypiv IV

Sun Apr 01 00:00:00 EDT 2018

Stromally expressed β-catenin modulates Wnt9b signaling in the ureteric epithelium.

PloS one

Boivin FJ,Sarin S,Lim J,Javidan A,Svajger B,Khalili H,Bridgewater D

Thu Feb 25 00:00:00 EST 2016

Ablation of the renal stroma defines its critical role in nephron progenitor and vasculature patterning.

PloS one

Hum S,Rymer C,Schaefer C,Bushnell D,Sims-Lucas S

Mon Sep 29 00:00:00 EDT 2014

Renal blood flow and oxygenation drive nephron progenitor differentiation.

American journal of physiology. Renal physiology

Rymer C,Paredes J,Halt K,Schaefer C,Wiersch J,Zhang G,Potoka D,Vainio S,Gittes GK,Bates CM,Sims-Lucas S

Fri Aug 01 00:00:00 EDT 2014

Mdm2 is required for maintenance of the nephrogenic niche.

Developmental biology

Hilliard SA,Yao X,El-Dahr SS

Sat Mar 01 00:00:00 EST 2014

Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation.

Nature cell biology

Das A,Tanigawa S,Karner CM,Xin M,Lum L,Chen C,Olson EN,Perantoni AO,Carroll TJ

Sun Sep 01 00:00:00 EDT 2013

Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation.

Nature cell biology

Das A,Tanigawa S,Karner CM,Xin M,Lum L,Chen C,Olson EN,Perantoni AO,Carroll TJ

Sun Sep 01 00:00:00 EDT 2013

Canonical Wnt9b signaling balances progenitor cell expansion and differentiation during kidney development.

Development (Cambridge, England)

Karner CM,Das A,Ma Z,Self M,Chen C,Lum L,Oliver G,Carroll TJ

Fri Apr 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: Amphiphysin; amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen); amphiphysin 1; amphiphysin I; HGNC:471

Gene Aliases: AMPH; AMPH1

UniProt ID: (Human) P49418, (Rat) O08838, (Mouse) Q7TQF7

Entrez Gene ID: (Human) 273, (Rat) 60668, (Mouse) 218038

learning positive regulation of GTPase activity positive regulation of endocytosis synaptic vesicle endocytosis endocytosis chemical synaptic transmission

Process(es)

ARF guanyl-nucleotide exchange factor activity protein binding phospholipid binding protein C-terminus binding protein complex binding protein heterodimerization activity