|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 737-751 of human APP.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.02% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||1/500 - 1/1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-84165 recognizes both intact APP and also the C99 fragment generated by Beta-secretase. The sequence recognized by this antibody corresponds to amino acids 85-99 of the C99 fragment.
Heat-mediated antigen retrieval is recommended for the staining of paraffin sections.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Alzheimer's disease related markers, cellular toxicity and behavioral deficits induced six weeks after oligomeric amyloid-ß peptide injection in rats.
PA1-84165 was used in western blot to study the ability of a single intracerebroventricular injection of soluble oligomers of a beta-amyloid fragment to induce Alzhiemers's disease pathology in a rat model
|Zussy C,Brureau A,Keller E,Marchal S,Blayo C,Delair B,Ixart G,Maurice T,Givalois L||PloS one (8:null)||2013|