Invitrogen

Amyloid Precursor Protein Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (7)
Advanced Verification (3)

Amyloid Precursor Protein Antibody in Western Blot (WB)

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Amyloid Precursor Protein Antibody (PA5-17829) in WB

Western blot was performed using Anti-beta Amyloid Polyclonal Antibody (Product # PA5-17829) and ~~120, 90 kDa bands corresponding to beta Amyloid was observed across the panel tested except for K-562, HEL 92.1.7, Mouse Skeletal Muscle and Rat Skeletal Muscle which are reported to be negative. Whole cell extracts (30 µg lysate) of PC-3 (Lane 1), SH-SY5Y (Lane 2), K-562 (Lane 3) and HEL 92.1.7 (Lane 4) (Fig.... View More

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Amyloid Precursor Protein Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of beta Amyloid was performed using 70% confluent log phase PC-3. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with beta Amyloid Polyclonal Antibody (Product # PA5-17829) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization in plasma membrane, cytoplasm and nucleus. Panel e represents negative cell line K-562 with no expression. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Amyloid Precursor Protein Antibody in Immunoprecipitation (IP) — Immunoprecipitation of APP was performed on HEK293T cells. Antigen-antibody complexes were formed by incubating 500 µg of HEK293T whole cell lysate (in 500 µL volume) with 5 µL of an APP polyclonal antibody (Product # PA5-17829) overnight at 4°C. The immune complexes were captured on 30 µL of protein G agarose, washed extensively, and eluted with 6X Laemmli buffer. Samples were resolved on an 8% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with an APP polyclonal antibody (Product # PA5-17829) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated mouse anti-rabbit light chain specific secondary antibody at a dilution of 1:40,000 for 1 hour at room temperature. Chemiluminescent detection was performed using ECL substrate. Data courtesy of the Innovators Program.

Amyloid Precursor Protein Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues tested owing to their inherent genetic constitution. Relative expression of Amyloid Precursor Protein was observed in PC-3, SH-SY5Y, Mouse Brain and Rat Brain in comparison to K-562, HEL 92.1.7, Mouse Skeletal Muscle and Rat Skeletal Muscle using Anti-beta Amyloid Polyclonal antibody (Product # PA5-17829) in Western Blot. {RE}

Product Details

PA5-17829

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunocytochemistry (ICC/IF)

1:50-1:200

Immunoprecipitation (IP)

2-5 µL

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues surrounding Thr668 of human APP695

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

197 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 100µg/mL BSA

Contains

no preservative

Storage conditions

-20°C

RRID

AB_10982330

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Amyloid Precursor Protein (APP) or Amyloid beta precursor protein functions as a cell surface kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. APP is important for neurite growth, neuronal adhesion and axonogenesis. APP is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms resulting from alternative splicing. Proteolytic cleavage of APP by beta- and gamma-secretases results in the generation of beta amyloid, which is the primary component of senile plaques. Senile plaques are one of the major histopathologic features of Alzheimer's disease. Abnormal regulation and processing of APP also plays a role in Down's syndrome, early onset familial Alzheimer's disease, and cerebral hemorrhage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Abeta40; Abeta42; ABPP; AG; AICD-50; AICD-57; AICD-59; AID(50); AID(57); AID(59); Alpha-CTF; Alpha-secretase C-terminal fragment; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; amyloid A4; Amyloid b precursor protein; amyloid beta (A4) precursor protein; amyloid beta A4 protein; Amyloid intracellular domain 50; Amyloid intracellular domain 57; Amyloid intracellular domain 59; Amyloid precursor protein; Amyloid β precursor protein; Amyloid-beta (A4) precursor protein; Amyloid-beta A4 protein; Amyloid-beta precursor protein; Amyloid-beta protein 40; Amyloid-beta protein 42; Amyloidogenic glycoprotein; APP; APP-C57; APP-C59; APP-C99; APPI; appican; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); beta-amyloid precursor protein; Beta-APP40; Beta-APP42; Beta-CTF; Beta-secretase C-terminal fragment; C31; C80; C83; C99; Cerebral vascular amyloid peptide; CVAP; Gamma-CTF(50); Gamma-CTF(57); Gamma-CTF(59); Gamma-secretase C-terminal fragment 50; Gamma-secretase C-terminal fragment 57; Gamma-secretase C-terminal fragment 59; N-APP; P3(40); P3(42); Pan-Abeta; peptidase nexin-II; PN-II; PreA4; protease nexin II; Protease nexin-II; S-APP-alpha; S-APP-beta; Soluble APP-alpha; Soluble APP-beta; testicular tissue protein Li 2

Gene Aliases: A4; AAA; ABETA; ABPP; AD1; Adap; Ag; APP; APPI; betaApp; CTFgamma; CVAP; E030013M08Rik; PN-II; PN2

UniProt ID: (Human) P05067, (Rat) P08592, (Mouse) P12023

Entrez Gene ID: (Human) 351, (Rat) 54226, (Mouse) 11820

Molecular Function: protease inhibitor