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          • Primary Antibodies ›
          • Amyloid Precursor Protein Antibodies

          Invitrogen

          Amyloid Precursor Protein Polyclonal Antibody

          View all (98) Amyloid Precursor Protein antibodies

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          Cite Amyloid Precursor Protein Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (5)
          Amyloid Precursor Protein Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Amyloid Precursor Protein Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          Amyloid Precursor Protein Antibody (PA5-34657) in ICC/IF

          Immunocytochemistry-Immunofluorescence analysis of beta Amyloid was performed in SK N SH cells fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Amyloid Polyclonal Antibody (Product # PA5 34657) diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 µm. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          Amyloid Precursor Protein Antibody in Immunocytochemistry (ICC/IF)
          Amyloid Precursor Protein Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Amyloid Precursor Protein Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Amyloid Precursor Protein Antibody in Western Blot (WB)
          Amyloid Precursor Protein Antibody in Western Blot (WB)
          Amyloid Precursor Protein Polyclonal Antibody

          Product Details

          PA5-34657

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:1,000
          -

          Immunohistochemistry (Frozen) (IHC (F))

          Assay-dependent
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the C-terminus region of human APP. The exact sequence is proprietary.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7, with 10% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2552009

          Product Specific Information

          Recommended positive controls: HepG2, NIH-3T3, U87-MG, SK-N-SH, IMR32, SK-N-AS.

          Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (93%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (98%), Chimpanzee (100%), Bovine (100%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          Amyloid Precursor Protein (APP) or Amyloid beta precursor protein functions as a cell surface kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. APP is important for neurite growth, neuronal adhesion and axonogenesis. APP is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms resulting from alternative splicing. Proteolytic cleavage of APP by beta- and gamma-secretases results in the generation of beta amyloid, which is the primary component of senile plaques. Senile plaques are one of the major histopathologic features of Alzheimer's disease. Abnormal regulation and processing of APP also plays a role in Down's syndrome, early onset familial Alzheimer's disease, and cerebral hemorrhage.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: ABPP; AG; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; amyloid A4; Amyloid b precursor protein; amyloid beta (A4) precursor protein; amyloid beta A4 protein; amyloid beta precursor protein (ABPP); amyloid beta protein; amyloid beta-protein precursor; Amyloid precursor protein; amyloid protein; Amyloid β precursor protein; Amyloid-beta (A4) precursor protein; Amyloid-beta A4 protein; Amyloid-beta precursor protein; Amyloidogenic glycoprotein; APP; APPI; appican; beta-amyloid A4; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); beta-amyloid precursor protein; Cerebral vascular amyloid peptide; CVAP; Pan-Abeta; peptidase nexin-II; PN-II; PreA4; protease nexin II; Protease nexin-II; testicular tissue protein Li 2; unnamed protein product

          View more View less

          Gene Aliases: A4; AAA; ABETA; ABPP; AD1; Adap; Ag; alpha-sAPP; APP; APPI; betaApp; CTFgamma; CVAP; E030013M08Rik; PN-II; PN2; preA4

          View more View less

          UniProt ID: (Human) B2R5V1, (Rat) P08592, (Mouse) P12023

          View more View less

          Entrez Gene ID: (Human) 351, (Rat) 54226, (Mouse) 11820

          View more View less

          Function(s)
          G-protein coupled receptor binding DNA binding chromatin binding serine-type endopeptidase inhibitor activity receptor binding frizzled binding insulin receptor binding integrin binding protein binding heparin binding peptidase activator activity enzyme binding peptidase inhibitor activity receptor activator activity acetylcholine receptor activator activity acetylcholine receptor binding apolipoprotein binding chemoattractant activity identical protein binding protein homodimerization activity heparan sulfate proteoglycan binding metal ion binding ephrin receptor binding transition metal ion binding protein heterodimerization activity receptor agonist activity low-density lipoprotein particle receptor binding RAGE receptor binding chaperone binding PTB domain binding growth factor receptor binding amylin binding protein serine/threonine kinase binding molecular function activator activity heparan sulfate binding protease inhibitor
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter microglial cell activation response to yeast astrocyte activation involved in immune response regulation of transcription from RNA polymerase II promoter regulation of translation cellular copper ion homeostasis endocytosis apoptotic process cell adhesion G-protein coupled receptor signaling pathway adenylate cyclase-activating G-protein coupled receptor signaling pathway adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Notch signaling pathway chemical synaptic transmission axonogenesis central nervous system development learning or memory learning memory mating behavior locomotory behavior axo-dendritic transport negative regulation of cell proliferation associative learning adult locomotory behavior visual learning response to lead ion regulation of gene expression positive regulation of macromolecule metabolic process positive regulation of gene expression negative regulation of gene expression response to auditory stimulus microglia development axon midline choice point recognition neuron remodeling dendrite development calcium-mediated signaling antibacterial humoral response antifungal humoral response regulation of Wnt signaling pathway extracellular matrix organization neuron projection development phospholipase D-activating G-protein coupled receptor signaling pathway positive regulation of chemokine production positive regulation of interferon-gamma production positive regulation of interleukin-1 beta production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production positive regulation of superoxide anion generation regulation of toll-like receptor signaling pathway ionotropic glutamate receptor signaling pathway regulation of multicellular organism growth lipoprotein metabolic process positive regulation of apoptotic process positive regulation of MAPK cascade positive regulation of neuron apoptotic process innate immune response positive regulation of nitric oxide biosynthetic process positive regulation of neuron differentiation positive regulation of G-protein coupled receptor protein signaling pathway positive regulation of Toll signaling pathway positive regulation of glycolytic process positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade astrocyte activation regulation of long-term neuronal synaptic plasticity collateral sprouting in absence of injury positive regulation of inflammatory response regulation of synapse structure or activity synapse organization defense response to Gram-negative bacterium defense response to Gram-positive bacterium positive regulation of calcium-mediated signaling positive regulation of cell activation cognition positive chemotaxis positive regulation of membrane protein ectodomain proteolysis regulation of protein metabolic process positive regulation of protein metabolic process protein homooligomerization protein tetramerization regulation of neurotransmitter uptake positive regulation of protein kinase B signaling low-density lipoprotein particle mediated signaling antimicrobial humoral immune response mediated by antimicrobial peptide protein trimerization positive regulation of ERK1 and ERK2 cascade response to interleukin-1 cellular response to copper ion cellular response to manganese ion cellular response to cAMP cellular response to norepinephrine stimulus positive regulation of monocyte chemotaxis negative regulation of canonical Wnt signaling pathway modulation of excitatory postsynaptic potential NMDA selective glutamate receptor signaling pathway positive regulation of gene expression, epigenetic positive regulation of cAMP/PKA signal transduction regulation of spontaneous synaptic transmission negative regulation of protein localization to nucleus regulation of beta-amyloid clearance negative regulation of long-term synaptic potentiation positive regulation of long-term synaptic potentiation positive regulation of NIK/NF-kappaB signaling regulation of calcium ion transmembrane transport via high voltage-gated calcium channel negative regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of dendritic spine maintenance positive regulation of amyloid precursor protein catabolic process regulation of endoplasmic reticulum stress-induced neuron intrinsic apoptotic signaling pathway negative regulation of blood circulation positive regulation of G protein-coupled receptor internalization positive regulation of endothelin production positive regulation of protein import cellular response to beta-amyloid regulation of presynapse assembly positive regulation of response to endoplasmic reticulum stress regulation of amyloid fibril formation positive regulation of amyloid fibril formation regulation of response to calcium ion amyloid fibril formation cellular response to nerve growth factor stimulus neuron projection maintenance positive regulation of T cell migration positive regulation of excitatory postsynaptic potential positive regulation of extrinsic apoptotic signaling pathway suckling behavior mRNA polyadenylation protein phosphorylation response to oxidative stress regulation of epidermal growth factor-activated receptor activity cholesterol metabolic process negative regulation of endopeptidase activity positive regulation of peptidase activity positive regulation of G2/M transition of mitotic cell cycle forebrain development regulation of protein binding negative regulation of neuron differentiation neuromuscular process controlling balance synaptic growth at neuromuscular junction neuron apoptotic process smooth endoplasmic reticulum calcium ion homeostasis nervous system development negative regulation of peptidase activity
          It has to be done as per old AB suggested Products section.

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