Immunohistochemistry analysis of Androgen Receptor showing staining in the cytoplasm and nucleus of paraffin-treated human prostate tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Androgen Receptor polyclonal antibody (Product # PA1-110) diluted by 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Rat, Pig, Hamster, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion protein containing residues 331-572 of human androgen receptor.|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:10-1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 16 publications below|
|ChIP assay (ChIP)||See 1 publications below|
|Western Blot (WB)||See 3 publications below|
|Immunoprecipitation (IP)||See 3 publications below|
|Immunocytochemistry (ICC)||See 4 publications below|
PA1-110 detects androgen receptor (AR) from human, primate, rat, and mouse tissues. This antibody does not detect estrogen, progesterone, or glucocorticoid receptors.
PA1-110 has been successfully used in Western blot, immunocytochemistry, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~110 kDa protein representing AR from rat brain homogenate. Immunohistochemical staining of AR in rat brain with PA1-110 results in intense nuclear staining.
For fixation of frozen sections, 2% paraformaldehyde + 15% picric acid solution in phosphate buffer, pH 7.4 is recommended.
The PA1-110 immunogen is a fusion protein containing residues 331-572 of human androgen receptor.
Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Androgen receptors (AR) have been found in a variety of tissues, including reproductive organs, central nervous system and skeletal muscle.
The corresponding gene for the androgen receptor is NR3C4.
IP-MS enrichment of AR (LFQ intensity): AR was enriched 551-fold from LNCAP lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and AR antibody (Part No. PA1-110). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Testosterone and reward prediction-errors in healthy men and men with schizophrenia.
PA1-110 was used in immunohistochemistry - frozen section to test if testosterone can have a direct effect on dopamine neurons
|Morris RW,Purves-Tyson TD,Weickert CS,Rothmond D,Lenroot R,Weickert TW||Schizophrenia research (168:649)||2015|
High fidelity patient-derived xenografts for accelerating prostate cancer discovery and drug development.
PA1-110 was used in immunohistochemistry to establish a set of transplantable xenografts from prostate cancer patients with the potential to aid drug discovery and development
|Lin D,Wyatt AW,Xue H,Wang Y,Dong X,Haegert A,Wu R,Brahmbhatt S,Mo F,Jong L,Bell RH,Anderson S,Hurtado-Coll A,Fazli L,Sharma M,Beltran H,Rubin M,Cox M,Gout PW,Morris J,Goldenberg L,Volik SV,Gleave ME,Collins CC,Wang Y||Cancer research (74:1272)||2014|
Deletion of leucine zipper tumor suppressor 2 (Lzts2) increases susceptibility to tumor development.
PA1-110 was used in immunohistochemistry to study the increased tumor susceptibility of mice ablated for Lzts2
|Johnson DT,Luong R,Lee SH,Peng Y,Shaltouki A,Lee JT,Lin D,Wang Y,Sun Z||The Journal of biological chemistry (288:3727)||2013|
Androgen deprivation by flutamide modulates uPAR, MMP-9 expressions, lipid profile, and oxidative stress: amelioration by daidzein.
PA1-110 was used in immunohistochemistry to study the beneficial effect of daidzein on the changes in uPAR, MMP-9, lipid metabolism and oxidative stress induced by flutamide androgen deprivation therapy
|Lateef A,Khan AQ,Tahir M,Khan R,Rehman MU,Ali F,Hamiza OO,Sultana S||Molecular and cellular biochemistry (374:49)||2013|
Differential effects of androgens on coronary blood flow regulation and arteriolar diameter in intact and castrated swine.
PA1-110 was used in immunohistochemistry to study the effect of androgens on coronary blood flow regulation and arteriolar diameter in intact and castrated swine
|O'Connor EK,Ivey JR,Bowles DK||Biology of sex differences (3:null)||2012|
Testosterone regulation of sex steroid-related mRNAs and dopamine-related mRNAs in adolescent male rat substantia nigra.
PA1-110 was used in immunohistochemistry to study testosterone regulation of sex steroid-related mRNAs and dopamine-related mRNAs in adolescent male rat substantia nigra
|Purves-Tyson TD,Handelsman DJ,Double KL,Owens SJ,Bustamante S,Weickert CS||BMC neuroscience (13:null)||2012|
Sex hormones receptors play a crucial role in the control of femoral and mandibular growth in newborn mice.
PA1-110 was used in immunohistochemistry to investigate the effect of sex hormones receptors on bone development in neonatal mice
|Márquez Hernández RA,Ohtani J,Fujita T,Sunagawa H,Kawata T,Kaku M,Motokawa M,Tanne K||European journal of orthodontics (33:564)||2011|
Induction of apoptosis in the LNCaP human prostate carcinoma cell line and prostate adenocarcinomas of SV40T antigen transgenic rats by the Bowman-Birk inhibitor.
PA1-110 was used in immunohistochemistry to investigate the influence of Bowman-Birk inhibitor on cell apoptosis in prostate cancer
|Tang M,Asamoto M,Ogawa K,Naiki-Ito A,Sato S,Takahashi S,Shirai T||Pathology international (59:790)||2009|
Inhibition of prostate carcinogenesis in probasin/SV40 T antigen transgenic rats by leuprorelin, a luteinizing hormone-releasing hormone agonist.
PA1-110 was used in immunohistochemistry to investigate the inhibitory effect of leuprorelin acetate on prostate carcinogenesis in a rat model
|Said MM,Hokaiwado N,Tang M,Ogawa K,Suzuki S,Ghanem HM,Esmat AY,Asamoto M,Refaie FM,Shirai T||Cancer science (97:459)||2006|
Endogenous testosterone increases L-type Ca2+ channel expression in porcine coronary smooth muscle.
PA1-110 was used in immunohistochemistry to investigate the possible effect of endogenous testosterone on regulation of CASM L-type voltage-gated calcium current by means of regulation of VGCC expression and activity
|Bowles DK,Maddali KK,Ganjam VK,Rubin LJ,Tharp DL,Turk JR,Heaps CL||American journal of physiology. Heart and circulatory physiology (287:H2091)||2004|
Role of p63 and basal cells in the prostate.
PA1-110 was used in immunohistochemistry to study the function of p63 and basal cells in the prostate
|Kurita T,Medina RT,Mills AA,Cunha GR||Development (Cambridge, England) (131:4955)||2004|
hZimp10 is an androgen receptor co-activator and forms a complex with SUMO-1 at replication foci.
PA1-110 was used in immunohistochemistry to study the role of a novel PIAS-like protein, hZimp10, in transcription and DNA replication.
|Sharma M,Li X,Wang Y,Zarnegar M,Huang CY,Palvimo JJ,Lim B,Sun Z||The EMBO journal (22:6101)||2003|
Manifestation of Y-chromosomal deletions in the human testis: a morphometrical and immunohistochemical evaluation.
PA1-110 was used in immunohistochemistry to study the morphological parameters of testicular biopsies from 17 patients diagnosed for Y chromosome microdeletions
|Luetjens CM,Gromoll J,Engelhardt M,Von Eckardstein S,Bergmann M,Nieschlag E,Simoni M||Human reproduction (Oxford, England) (17:2258)||2002|
Effects of genetic background on prostate and taste bud carcinogenesis due to SV40 T antigen expression under probasin gene promoter control.
PA1-110 was used in immunohistochemistry to evaluate a transgenic rat model for studing prostate and taste bud carcinogenesis
|Asamoto M,Hokaiwado N,Cho YM,Shirai T||Carcinogenesis (23:463)||2002|
Actions of the endocrine disruptor methoxychlor and its estrogenic metabolite on in vitro embryonic rat seminiferous cord formation and perinatal testis growth.
PA1-110 was used in immunohistochemistry to study the effect of methoxychlor and its estrogenic metabolite on embryonic rat seminiferous cord formation and perinatal testis growth
|Cupp AS,Skinner MK||Reproductive toxicology (Elmsford, N.Y.) (15:317)||2001|
Sex differences in the distribution of androgen receptors in the human hypothalamus.
PA1-110 was used in immunohistochemistry to investigate the differential distribution of AR in human hypothalamus between sexes
|Fernández-Guasti A,Kruijver FP,Fodor M,Swaab DF||The Journal of comparative neurology (425:422)||2000|
Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line.
PA1-110 was used in immunohistochemistry to define a new immortalized human prostatic cell line
|Webber MM,Bello D,Kleinman HK,Wartinger DD,Williams DE,Rhim JS||Carcinogenesis (17:1641)||1996|
ETV1 is a novel androgen receptor-regulated gene that mediates prostate cancer cell invasion.
PA1-110 was used in ChIP assay to investigate the influence of ETV1 on cancer invasion
|Cai C,Hsieh CL,Omwancha J,Zheng Z,Chen SY,Baert JL,Shemshedini L||Molecular endocrinology (Baltimore, Md.) (21:1835)||2007|
c-Jun has multiple enhancing activities in the novel cross talk between the androgen receptor and Ets variant gene 1 in prostate cancer.
PA1-110 was used in western blot to study roles of c-Jun in a novel cross-talk between the androgen receptor (AR) and its new target gene, Ets variant gene 1
|Cai C,Hsieh CL,Shemshedini L||Molecular cancer research : MCR (5:725)||2007|
c-Jun enhancement of androgen receptor transactivation is associated with prostate cancer cell proliferation.
PA1-110 was used in western blot to investigate the role of c-Jun during androgen receptor transactivation and its relationship with prostate cancer cell proliferation
|Chen SY,Cai C,Fisher CJ,Zheng Z,Omwancha J,Hsieh CL,Shemshedini L||Oncogene (25:7212)||2006|
Androgenic up-regulation of androgen receptor cDNA expression in androgen-independent prostate cancer cells.
PA1-110 was used in western blot to study the androgen-mediated up-regulation of androgen receptor cDNA expression in androgen-independent prostate cancer cells
|Dai JL,Maiorino CA,Gkonos PJ,Burnstein KL||Steroids (61:531)||1996|
Androgen regulation of soluble guanylyl cyclasealpha1 mediates prostate cancer cell proliferation.
PA1-110 was used in immunoprecipitation to study how androgens regulate the proliferation of prostate cancer cell by means of the alpha 1-subunit gene of soluble guanylyl cyclase
|Cai C,Chen SY,Zheng Z,Omwancha J,Lin MF,Balk SP,Shemshedini L||Oncogene (26:1606)||2007|
Steroid-induced androgen receptor-oestradiol receptor beta-Src complex triggers prostate cancer cell proliferation.
PA1-110 was used in immunoprecipitation and western blot to study the role of the androgen receptor-oestradiol receptor beta-Src complex in triggering prostate cancer cell proliferation
|Migliaccio A,Castoria G,Di Domenico M,de Falco A,Bilancio A,Lombardi M,Barone MV,Ametrano D,Zannini MS,Abbondanza C,Auricchio F||The EMBO journal (19:5406)||2000|
BAG-1L protein enhances androgen receptor function.
PA1-110 was used in immunoprecipitation to study the regulatory role of the BAG-1L protein in androgen receptor fucntion
|Froesch BA,Takayama S,Reed JC||The Journal of biological chemistry (273:11660)||1998|
Modifications of testosterone-dependent behaviors by estrogen receptor-alpha gene disruption in male mice.
PA1-110 was used in immunocytochemistry to study the effect of estrogen receptor-alpha gene disruption on testosterone-dependent behaviors in male mice
|Ogawa S,Washburn TF,Taylor J,Lubahn DB,Korach KS,Pfaff DW||Endocrinology (139:5058)||1998|
Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18.
PA1-110 was used in immunocytochemistry to characterize human immortalized prostatic epithelial cell lines responsive to androgen
|Bello D,Webber MM,Kleinman HK,Wartinger DD,Rhim JS||Carcinogenesis (18:1215)||1997|
Androgen receptors in cultured rat adipose precursor cells during proliferation and differentiation: regional specificities and regulation by testosterone.
PA1-110 was used in immunocytochemistry to study the expression of androgen receptors in cultured rat adipocyte precursors isolated from intra-abdominal and subcutaneous fat depots
|Dieudonne MN,Pecquery R,Leneveu MC,Jaubert AM,Giudicelli Y||Endocrine (3:537)||1995|
Distribution of androgen receptor-like immunoreactivity in the brains of intact and castrated male hamsters.
PA1-110 was used in immunocytochemistry to investigate the distribution of androgen receptor-like immunoreactivity in the brains of intact and castrated male hamsters
|Clancy AN,Whitman C,Michael RP,Albers HE||Brain research bulletin (33:325)||1994|