Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunohistochemistry analysis of Androgen Receptor showing staining in the cytoplasm and nucleus of paraffin-treated human prostate tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Androgen Receptor polyclonal antibody (Product # PA1-110) diluted by 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Primate, Rat|
|Published species reactivity||Rat, Pig, Hamster, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion protein containing residues 331-572 of human androgen receptor.|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||1:10-1:100|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-110 detects androgen receptor (AR) from human, primate, rat, and mouse tissues. This antibody does not detect estrogen, progesterone, or glucocorticoid receptors.
PA1-110 has been successfully used in Western blot, immunocytochemistry, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~110 kDa protein representing AR from rat brain homogenate. Immunohistochemical staining of AR in rat brain with PA1-110 results in intense nuclear staining.
For fixation of frozen sections, 2% paraformaldehyde + 15% picric acid solution in phosphate buffer, pH 7.4 is recommended.
The PA1-110 immunogen is a fusion protein containing residues 331-572 of human androgen receptor.
Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Androgen receptors (AR) have been found in a variety of tissues, including reproductive organs, central nervous system and skeletal muscle.
The corresponding gene for the androgen receptor is NR3C4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
High fidelity patient-derived xenografts for accelerating prostate cancer discovery and drug development.
PA1-110 was used in immunohistochemistry to establish a set of transplantable xenografts from prostate cancer patients with the potential to aid drug discovery and development
|Lin D,Wyatt AW,Xue H,Wang Y,Dong X,Haegert A,Wu R,Brahmbhatt S,Mo F,Jong L,Bell RH,Anderson S,Hurtado-Coll A,Fazli L,Sharma M,Beltran H,Rubin M,Cox M,Gout PW,Morris J,Goldenberg L,Volik SV,Gleave ME,Collins CC,Wang Y||Cancer research (74:1272)||2014|
Deletion of leucine zipper tumor suppressor 2 (Lzts2) increases susceptibility to tumor development.
PA1-110 was used in immunohistochemistry to study the increased tumor susceptibility of mice ablated for Lzts2
|Johnson DT,Luong R,Lee SH,Peng Y,Shaltouki A,Lee JT,Lin D,Wang Y,Sun Z||The Journal of biological chemistry (288:3727)||2013|
Androgen deprivation by flutamide modulates uPAR, MMP-9 expressions, lipid profile, and oxidative stress: amelioration by daidzein.
PA1-110 was used in immunohistochemistry to study the beneficial effect of daidzein on the changes in uPAR, MMP-9, lipid metabolism and oxidative stress induced by flutamide androgen deprivation therapy
|Lateef A,Khan AQ,Tahir M,Khan R,Rehman MU,Ali F,Hamiza OO,Sultana S||Molecular and cellular biochemistry (374:49)||2013|
Differential effects of androgens on coronary blood flow regulation and arteriolar diameter in intact and castrated swine.
PA1-110 was used in immunohistochemistry to study the effect of androgens on coronary blood flow regulation and arteriolar diameter in intact and castrated swine
|O'Connor EK,Ivey JR,Bowles DK||Biology of sex differences (3:null)||2012|
Testosterone regulation of sex steroid-related mRNAs and dopamine-related mRNAs in adolescent male rat substantia nigra.
PA1-110 was used in immunohistochemistry to study testosterone regulation of sex steroid-related mRNAs and dopamine-related mRNAs in adolescent male rat substantia nigra
|Purves-Tyson TD,Handelsman DJ,Double KL,Owens SJ,Bustamante S,Weickert CS||BMC neuroscience (13:null)||2012|
Sex hormones receptors play a crucial role in the control of femoral and mandibular growth in newborn mice.
PA1-110 was used in immunohistochemistry to investigate the effect of sex hormones receptors on bone development in neonatal mice
|Márquez Hernández RA,Ohtani J,Fujita T,Sunagawa H,Kawata T,Kaku M,Motokawa M,Tanne K||European journal of orthodontics (33:564)||2011|
Induction of apoptosis in the LNCaP human prostate carcinoma cell line and prostate adenocarcinomas of SV40T antigen transgenic rats by the Bowman-Birk inhibitor.
PA1-110 was used in immunohistochemistry to investigate the influence of Bowman-Birk inhibitor on cell apoptosis in prostate cancer
|Tang M,Asamoto M,Ogawa K,Naiki-Ito A,Sato S,Takahashi S,Shirai T||Pathology international (59:790)||2009|
Inhibition of prostate carcinogenesis in probasin/SV40 T antigen transgenic rats by leuprorelin, a luteinizing hormone-releasing hormone agonist.
PA1-110 was used in immunohistochemistry to investigate the inhibitory effect of leuprorelin acetate on prostate carcinogenesis in a rat model
|Said MM,Hokaiwado N,Tang M,Ogawa K,Suzuki S,Ghanem HM,Esmat AY,Asamoto M,Refaie FM,Shirai T||Cancer science (97:459)||2006|
Endogenous testosterone increases L-type Ca2+ channel expression in porcine coronary smooth muscle.
PA1-110 was used in immunohistochemistry to investigate the possible effect of endogenous testosterone on regulation of CASM L-type voltage-gated calcium current by means of regulation of VGCC expression and activity
|Bowles DK,Maddali KK,Ganjam VK,Rubin LJ,Tharp DL,Turk JR,Heaps CL||American journal of physiology. Heart and circulatory physiology (287:H2091)||2004|
Role of p63 and basal cells in the prostate.
PA1-110 was used in immunohistochemistry to study the function of p63 and basal cells in the prostate
|Kurita T,Medina RT,Mills AA,Cunha GR||Development (Cambridge, England) (131:4955)||2004|
hZimp10 is an androgen receptor co-activator and forms a complex with SUMO-1 at replication foci.
PA1-110 was used in immunohistochemistry to study the role of a novel PIAS-like protein, hZimp10, in transcription and DNA replication.
|Sharma M,Li X,Wang Y,Zarnegar M,Huang CY,Palvimo JJ,Lim B,Sun Z||The EMBO journal (22:6101)||2003|
Manifestation of Y-chromosomal deletions in the human testis: a morphometrical and immunohistochemical evaluation.
PA1-110 was used in immunohistochemistry to study the morphological parameters of testicular biopsies from 17 patients diagnosed for Y chromosome microdeletions
|Luetjens CM,Gromoll J,Engelhardt M,Von Eckardstein S,Bergmann M,Nieschlag E,Simoni M||Human reproduction (Oxford, England) (17:2258)||2002|
Effects of genetic background on prostate and taste bud carcinogenesis due to SV40 T antigen expression under probasin gene promoter control.
PA1-110 was used in immunohistochemistry to evaluate a transgenic rat model for studing prostate and taste bud carcinogenesis
|Asamoto M,Hokaiwado N,Cho YM,Shirai T||Carcinogenesis (23:463)||2002|
Actions of the endocrine disruptor methoxychlor and its estrogenic metabolite on in vitro embryonic rat seminiferous cord formation and perinatal testis growth.
PA1-110 was used in immunohistochemistry to study the effect of methoxychlor and its estrogenic metabolite on embryonic rat seminiferous cord formation and perinatal testis growth
|Cupp AS,Skinner MK||Reproductive toxicology (Elmsford, N.Y.) (15:317)||2001|
Sex differences in the distribution of androgen receptors in the human hypothalamus.
PA1-110 was used in immunohistochemistry to investigate the differential distribution of AR in human hypothalamus between sexes
|Fernández-Guasti A,Kruijver FP,Fodor M,Swaab DF||The Journal of comparative neurology (425:422)||2000|
Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line.
PA1-110 was used in immunohistochemistry to define a new immortalized human prostatic cell line
|Webber MM,Bello D,Kleinman HK,Wartinger DD,Williams DE,Rhim JS||Carcinogenesis (17:1641)||1996|
ETV1 is a novel androgen receptor-regulated gene that mediates prostate cancer cell invasion.
PA1-110 was used in ChIP assay to investigate the influence of ETV1 on cancer invasion
|Cai C,Hsieh CL,Omwancha J,Zheng Z,Chen SY,Baert JL,Shemshedini L||Molecular endocrinology (Baltimore, Md.) (21:1835)||2007|
c-Jun has multiple enhancing activities in the novel cross talk between the androgen receptor and Ets variant gene 1 in prostate cancer.
PA1-110 was used in western blot to study roles of c-Jun in a novel cross-talk between the androgen receptor (AR) and its new target gene, Ets variant gene 1
|Cai C,Hsieh CL,Shemshedini L||Molecular cancer research : MCR (5:725)||2007|
c-Jun enhancement of androgen receptor transactivation is associated with prostate cancer cell proliferation.
PA1-110 was used in western blot to investigate the role of c-Jun during androgen receptor transactivation and its relationship with prostate cancer cell proliferation
|Chen SY,Cai C,Fisher CJ,Zheng Z,Omwancha J,Hsieh CL,Shemshedini L||Oncogene (25:7212)||2006|
Androgenic up-regulation of androgen receptor cDNA expression in androgen-independent prostate cancer cells.
PA1-110 was used in western blot to study the androgen-mediated up-regulation of androgen receptor cDNA expression in androgen-independent prostate cancer cells
|Dai JL,Maiorino CA,Gkonos PJ,Burnstein KL||Steroids (61:531)||1996|
Androgen regulation of soluble guanylyl cyclasealpha1 mediates prostate cancer cell proliferation.
PA1-110 was used in immunoprecipitation to study how androgens regulate the proliferation of prostate cancer cell by means of the alpha 1-subunit gene of soluble guanylyl cyclase
|Cai C,Chen SY,Zheng Z,Omwancha J,Lin MF,Balk SP,Shemshedini L||Oncogene (26:1606)||2007|
Steroid-induced androgen receptor-oestradiol receptor beta-Src complex triggers prostate cancer cell proliferation.
PA1-110 was used in immunoprecipitation and western blot to study the role of the androgen receptor-oestradiol receptor beta-Src complex in triggering prostate cancer cell proliferation
|Migliaccio A,Castoria G,Di Domenico M,de Falco A,Bilancio A,Lombardi M,Barone MV,Ametrano D,Zannini MS,Abbondanza C,Auricchio F||The EMBO journal (19:5406)||2000|
BAG-1L protein enhances androgen receptor function.
PA1-110 was used in immunoprecipitation to study the regulatory role of the BAG-1L protein in androgen receptor fucntion
|Froesch BA,Takayama S,Reed JC||The Journal of biological chemistry (273:11660)||1998|
Modifications of testosterone-dependent behaviors by estrogen receptor-alpha gene disruption in male mice.
PA1-110 was used in immunocytochemistry to study the effect of estrogen receptor-alpha gene disruption on testosterone-dependent behaviors in male mice
|Ogawa S,Washburn TF,Taylor J,Lubahn DB,Korach KS,Pfaff DW||Endocrinology (139:5058)||1998|
Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18.
PA1-110 was used in immunocytochemistry to characterize human immortalized prostatic epithelial cell lines responsive to androgen
|Bello D,Webber MM,Kleinman HK,Wartinger DD,Rhim JS||Carcinogenesis (18:1215)||1997|
Androgen receptors in cultured rat adipose precursor cells during proliferation and differentiation: regional specificities and regulation by testosterone.
PA1-110 was used in immunocytochemistry to study the expression of androgen receptors in cultured rat adipocyte precursors isolated from intra-abdominal and subcutaneous fat depots
|Dieudonne MN,Pecquery R,Leneveu MC,Jaubert AM,Giudicelli Y||Endocrine (3:537)||1995|
Distribution of androgen receptor-like immunoreactivity in the brains of intact and castrated male hamsters.
PA1-110 was used in immunocytochemistry to investigate the distribution of androgen receptor-like immunoreactivity in the brains of intact and castrated male hamsters
|Clancy AN,Whitman C,Michael RP,Albers HE||Brain research bulletin (33:325)||1994|
ANDR; androgen nuclear receptor variant 2; androgen receptor; androgen receptor (Testicular feminization), same as Tfm; androgen receptor splice variant 4b; dihydrotestosterone receptor; nuclear receptor subfamily 3 group C member 4
AIS; Andr; AR; AR8; AW320017; DHTR; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM