Immunofluorescence analysis of Androgen Receptor was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Androgen Receptor Rabbit Polyclonal Antibody (PA1111B) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing punctate nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) E V Q L G L G R V Y P R P P S K T Y R G(21) C of human AR.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Western Blot (WB)||2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-111B detects androgen receptor (AR) from human samples. This antibody does not detect estrogen, progesterone, or glucocorticoid receptors.
PA1-111B has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~110 kDa protein representing AR from HepG2 nuclear extracts.
The PA1-111B immunogen is a synthetic peptide corresponding to residues M(1) E V Q L G L G R V Y P R P P S K T Y R G(21) C of human AR. This sequence is completely conserved between human, mouse, and rat AR. The PA1-111B immunizing peptide (Cat. # PEP-006) is available for use in neutralization and control experiments.
Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Androgen receptors (AR) have been found in a variety of tissues, including reproductive organs, central nervous system and skeletal muscle.
The corresponding gene for the androgen receptor is NR3C4.
IP-MS enrichment of AR (LFQ intensity): AR was enriched 229-fold from LNCAP lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and AR antibody (Part No. PA1-111B). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Effects of in utero exposure to di(n-butyl) phthalate for estrogen receptors ¿, ß, and androgen receptor of Leydig cell on rats.
PA1-111B was used in immunohistochemistry and western blot to study the expression of ER-alpha, ER-beta and AR in rat Leydig cells following in utero di(n-butyl) phthalate exposure
|Wakui S,Shirai M,Motohashi M,Mutou T,Oyama N,Wempe MF,Takahashi H,Inomata T,Ikegami M,Endou H,Asari M||Toxicologic pathology (42:877)||2014|
The role of androgen-induced growth factor (FGF8) on genital tubercle development in a hypospadiac male rat model of prenatal exposure to di-n-butyl phthalate.
PA1-111B was used in western blot to study the role of androgen-induced growth factor in genital tubercle development developmental in hypospadiac male rats
|Liu SB,Ma Z,Sun WL,Sun XW,Hong Y,Ma L,Qin C,Stratton HJ,Liu Q,Jiang JT||Toxicology (293:53)||2012|
Influence of atrazine administration and reduction of calorie intake on prostate carcinogenesis in probasin/SV40 T antigen transgenic rats.
PA1-111B was used in immunohistochemistry to detect the effects of atrazine exposure on the development of androgen dependent prostate carcinoma
|Kandori H,Suzuki S,Asamoto M,Murasaki T,Mingxi T,Ogawa K,Shirai T||Cancer science (96:221)||2005|
Liganded androgen receptor interaction with beta-catenin: nuclear co-localization and modulation of transcriptional activity in neuronal cells.
PA1-111B was used in immunocytochemistry to investigate the association between beta-catenin and androgen receptor.
|Pawlowski JE,Ertel JR,Allen MP,Xu M,Butler C,Wilson EM,Wierman ME||The Journal of biological chemistry (277:20702)||2002|