PA1-111B detects androgen receptor (AR) from human samples. This antibody does not detect estrogen, progesterone, or glucocorticoid receptors.
PA1-111B has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~110 kDa protein representing AR from HepG2 nuclear extracts.
The PA1-111B immunogen is a synthetic peptide corresponding to residues M(1) E V Q L G L G R V Y P R P P S K T Y R G(21) C of human AR. This sequence is completely conserved between human, mouse, and rat AR. The PA1-111B immunizing peptide (Cat. # PEP-006) is available for use in neutralization and control experiments.
The androgen receptor (AR) is a member of the steroid-hormone receptor superfamily of nuclear receptors. The receptor is more than 90 kDa and has three major functional domains: the N-terminal domain, DNA-binding domain, and the androgen-binding domain. The androgen receptor is a ligand-activated transcription factor that binds active testosterone (T) and dihydrotestosterone (DHT). Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Androgen Receptor is a phosphoprotein, and also regulates mitogen-activated protein kinase (MAP kinase). The inhibition of the MEK1/2 pathway correlates directly with a change in phosphorylation state of the androgen receptor. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease, and male infertility. Mutations in this gene are associated with complete androgen insensitivity (CAIS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: androgen nuclear receptor variant 2; Androgen receptor; androgen receptor splice variant 4b; AR-A; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4; RP11-383C12.1; Variant AR45
Gene Aliases: AIS; AR; AR8; DHTR; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM
UniProt ID: (Human) P10275
Entrez Gene ID: (Human) 367