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Immunofluorescence analysis of Androgen Receptor was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Androgen Receptor Rabbit Polyclonal Antibody (PA1111B) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing punctate nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) E V Q L G L G R V Y P R P P S K T Y R G(21) C of human AR.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Western Blot (WB)||2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-111B detects androgen receptor (AR) from human samples. This antibody does not detect estrogen, progesterone, or glucocorticoid receptors.
PA1-111B has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~110 kDa protein representing AR from HepG2 nuclear extracts.
The PA1-111B immunogen is a synthetic peptide corresponding to residues M(1) E V Q L G L G R V Y P R P P S K T Y R G(21) C of human AR. This sequence is completely conserved between human, mouse, and rat AR. The PA1-111B immunizing peptide (Cat. # PEP-006) is available for use in neutralization and control experiments.
Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Androgen receptors (AR) have been found in a variety of tissues, including reproductive organs, central nervous system and skeletal muscle.
The corresponding gene for the androgen receptor is NR3C4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Effects of in utero exposure to di(n-butyl) phthalate for estrogen receptors α, β, and androgen receptor of Leydig cell on rats.
PA1-111B was used in immunohistochemistry and western blot to study the expression of ER-alpha, ER-beta and AR in rat Leydig cells following in utero di(n-butyl) phthalate exposure
|Wakui S,Shirai M,Motohashi M,Mutou T,Oyama N,Wempe MF,Takahashi H,Inomata T,Ikegami M,Endou H,Asari M||Toxicologic pathology (42:877)||2014|
The role of androgen-induced growth factor (FGF8) on genital tubercle development in a hypospadiac male rat model of prenatal exposure to di-n-butyl phthalate.
PA1-111B was used in western blot to study the role of androgen-induced growth factor in genital tubercle development developmental in hypospadiac male rats
|Liu SB,Ma Z,Sun WL,Sun XW,Hong Y,Ma L,Qin C,Stratton HJ,Liu Q,Jiang JT||Toxicology (293:53)||2012|
Influence of atrazine administration and reduction of calorie intake on prostate carcinogenesis in probasin/SV40 T antigen transgenic rats.
PA1-111B was used in immunohistochemistry to detect the effects of atrazine exposure on the development of androgen dependent prostate carcinoma
|Kandori H,Suzuki S,Asamoto M,Murasaki T,Mingxi T,Ogawa K,Shirai T||Cancer science (96:221)||2005|
Liganded androgen receptor interaction with beta-catenin: nuclear co-localization and modulation of transcriptional activity in neuronal cells.
PA1-111B was used in immunocytochemistry to investigate the association between beta-catenin and androgen receptor.
|Pawlowski JE,Ertel JR,Allen MP,Xu M,Butler C,Wilson EM,Wierman ME||The Journal of biological chemistry (277:20702)||2002|
ANDR; androgen nuclear receptor variant 2; androgen receptor splice variant 4b; dihydrotestosterone receptor; nuclear receptor subfamily 3 group C member 4
AIS; AR; AR8; DHTR; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM