Immunofluorescent analysis of Androgen Receptor (green) in LNCaP (right panel) and negative control U2OS cells (left panel). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with an Androgen Receptor polyclonal antibody (Product # PA5-16750) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from N-terminal of rat AR|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Immunoprecipitation (IP)||2 ug|
|Western Blot (WB)||1:100-1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA5-16750 targets Androgen Receptor in IF, IHC (P), IP, and WB applications and shows reactivity with human, mouse, and rat samples.
The androgen receptor (AR) is a member of the steroid superfamily of ligand-dependent transcription factors. The expression of AR is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed as a marker of hormone-responsiveness.
IP-MS enrichment of AR (LFQ intensity): AR was enriched 1428-fold from LNCAP lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and AR antibody (Part No. PA5-16750). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Estradiol alleviates acinar cell apoptosis and chronic pancreatitis in male Wistar Bonn/Kobori rats.
PA5-16750 was used in immunohistochemistry to study the effects of estradiol on acinar cell apoptosis and chronic pancreatitis in male Wistar Bonn/Kobori rats
|Nakamura S,Yamada T,Hashimoto T,Takahashi S,Sogawa M,Ohara H,Nakazawa T,Sano H,Kuno A,Joh T,Nomura T,Arakawa T,Itoh M||Pancreas (26:e59)||2003|