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Invitrogen
Angiogenin Polyclonal Antibody
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FIGURE: 1 / 6
Angiogenin Antibody (PA5-78773) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of Angiogenin in U2OS cell using Angiogenin Polyclonal Antibody (Product # PA5-78773). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL and mouse anti-Beta Tubulin antibody overnight at 4°C. Cy3 conjugated goat anti-rabbit... View More
Product Details
PA5-78773
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human Angiogenin/ANG recombinant protein (Position: Q25-P147).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human hepatocellular carcinoma tumor tissue (HCCT). IHC: human hepatocellular carcinoma tissue, human lung cancer tissue. ICC/IF: U2OS cell. Flow: HepG2 cell.
Target Information
Angiogenin (ANG or ANG I) is important for the process of neovascularization and formation of new blood vessels. ANG is similar to pancreatic ribonuclease A and functions as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. It interacts with endothelial cell-surface actin and may cause changes in the cell cytoskeleton. ANG is thought to be involved in the development of solid tumors and its antagonists are capable of inhibiting tumor growth. Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9). Angiogenin is a genetic link between ALS and PD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Angiogenin; angiogenin, ribonuclease, RNase A family, 5; epididymis luminal protein 168; Homo sapiens epididymis luminal protein 168; MGC22466; MGC71966; Ribonuclease 5; ribonuclease A A1; ribonuclease A family member 5; RNase 5; RNASE4; unnamed protein product
Gene Aliases: ALS9; ANG; HEL168; RAA1; RNASE4; RNASE5
UniProt ID: (Human) P03950
Entrez Gene ID: (Human) 283
nucleic acid binding
DNA binding
actin binding
nuclease activity
endonuclease activity
endoribonuclease activity
ribonuclease activity
tRNA-specific ribonuclease activity
receptor binding
copper ion binding
protein binding
heparin binding
hydrolase activity
rRNA binding
peptide binding
protein homodimerization activity
ribosome binding
receptor agonist activity
angiogenesis
ovarian follicle development
oocyte maturation
response to hypoxia
response to yeast
placenta development
positive regulation of endothelial cell proliferation
cytoplasmic translation
receptor-mediated endocytosis
cell communication
signal transduction
rRNA transcription
response to hormone
tRNA catabolic process
cell migration
negative regulation of translation
antibacterial humoral response
antifungal humoral response
signaling
actin filament polymerization
cell differentiation
negative regulation of translation in response to stress
cellular response to stress
stress granule assembly
tRNA stabilization
positive regulation of phosphorylation
homeostatic process
negative regulation of apoptotic process
innate immune response
negative regulation of smooth muscle cell proliferation
positive regulation of protein secretion
defense response to Gram-positive bacterium
antimicrobial humoral immune response mediated by antimicrobial peptide
hematopoietic stem cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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