Immunofluorescent analysis of Angiostatin (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Angiostatin polyclonal antibody (Product # PA1-600) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: V(98) Y L S E C K T G I G N G Y R G T M S(116)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-600 detects recombinant human and mouse angiostatin.
PA1-600 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects a 50 kDa protein representing recombinant human angiostatin.
PA1-600 immunizing peptide corresponds to amino acid residues 98-116 from mouse angiostatin. This sequence is 95% and 84% conserved in rat and human angiostatin, respectively.
Angiostatin is a proteolytic fragment that comprises the first four kringle domains of the ~85 kDa plasminogen. Angiostatin reduces endothelial cell proliferation and acts as an potent inhibitor of angiogenesis and tumor growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Treatment of medulloblastoma with oncolytic measles viruses expressing the angiogenesis inhibitors endostatin and angiostatin.
PA1-600 was used in western blot to study the use of oncolytic measles viruses expressing endostatin and angiostatin for medulloblastoma therapy
|Hutzen B,Bid HK,Houghton PJ,Pierson CR,Powell K,Bratasz A,Raffel C,Studebaker AW||BMC cancer (14:null)||2014|