Reconstituted by adding 1 mL sterile distilled water.
The antiSerum does not cross-react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
In immunoelectrophoresis in agarose-plates use 2 µL human plasma or equivalent against 120 µL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 µL antiserum in 3 mm diameter center well and 2 µL plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Coagulation factor IX is a single chain polypeptide with a molecular mass of 57 kDa. Normal concentrations of factor IX in human plasma is approximately 3 µg/mL. Factor IX deficiency and lack of coagulation activity is seen in patients suffering from the innate X-chromosome linked disorder, haemophilia B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Christmas factor; Coagulation factor IX; Coagulation factor IXa heavy chain; Coagulation factor IXa light chain; EC 188.8.131.52; factor 9; factor IX F9; Factor9; FIX F9; plasma thromboplastic component; Plasma thromboplastin component; PTC; RP6-88D7.1; serine protease
Gene Aliases: F9; F9 p22; FIX; HEMB; P19; PTC; THPH8
UniProt ID: (Human) P00740
Entrez Gene ID: (Human) 2158
Molecular Function: serine protease