Immunohistochemical analysis of Apolipoprotein D in paraffin-embedded human kidney following steamed antigen retrieval with a citrate buffer, pH 6, using an Apolipoprotein D polyclonal antibody (Product# PA5-37837) at a dilution of 5ug/ml. Detection was performed using an AP-conjugated secondary antibody.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence EIEKIPTTFENGRC, from the internal region of APOD|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
|Western Blot (WB)||0.3-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is reported to work in a sandwich-ELISA as both as capture and detection antibody with Product # PA5-37838.
This gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. This glycoprotein is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism.
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