Immunofluorescent analysis of Arf6 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were probed with an Arf6 polyclonal antibody (Product # PA1-093) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Human, Mouse, Non-human primate|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||C-terminal human recombinant protein fragment|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
PA1-093X has been successfully used in Western blot, IHC (P) and immunofluorescence applications with human, mouse, and monkey samples.
ADP-ribosylation factor 6 (ARF6) is a guanine nucleotide-binding protein involved in protein trafficking and the regulation of endocytic recycling and cytoskeleton remodeling. As part of the RAS superfamily, Arf-6 stimulates the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. It may also be involved in dendritic spine development and the regulation of dendritic branching and filopodia extension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Secretion of soluble vascular endothelial growth factor receptor 1 (sVEGFR1/sFlt1) requires Arf1, Arf6, and Rab11 GTPases.
PA1-093X was used in immunocytochemistry to study the role of the Arf1, Arf6 and Rab11 GTPases in the secretion of soluble VEGFR-1
|Jung JJ,Tiwari A,Inamdar SM,Thomas CP,Goel A,Choudhury A||PloS one (7:null)||2012|