Invitrogen

Arginase 1 Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (53) Arginase 1 antibodies

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Questions & Answers

Datasheet

Questions & Answers

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Arginase 1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of Arginase 1 in human bone marrow using a Arginase 1 polyclonal antibody (Product # PA5-82106). The analysis shows strong cytoplasmic and nuclear positivity in a subset of hematopoietic cells.

Arginase 1 Antibody in Western Blot (WB) — Western blot analysis of Arginase 1 by a Arginase 1 polyclonal antibody (Product # PA5-82106). Analysis in human liver tissue.

Arginase 1 Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of Arginase 1 demonstrates antibody specificity. Immunohistochemical analysis of Arginase 1 using anti-Arginase 1 Polyclonal Antibody (Product #PA5-82106), shows significant staining of Arginase 1 in liver and shows minimal or weak staining in kidney tissues. The relative expression levels of Arginase 1 within each tissue is shown using RNA-Seq. {RE}

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:2500-1:5000

Western Blot (WB)

0.04-0.4 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human ARG1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2789267

Product Specific Information

Immunogen sequence: TIGIIGAPFS KGQPRGGVEE GPTVLRKAGL LEKLKEQECD VKDYGDLPFA DIPNDSPFQI VKNPRSVGKA SEQLAGKVAE VKKNGRISLV LGGDHSLAIG SISGHARVHP DLGVIWVDA

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: A-I; arginase 1 liver; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase

Gene Aliases: ARG1

UniProt ID: (Human) P05089

Entrez Gene ID: (Human) 383

Molecular Function: hydrolase