Invitrogen

Arginase 1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

3 Published Figures

3 References

View all (66) Arginase 1 antibodies

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Datasheet

Protocols

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Arginase 1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Arginase 1 in 4% paraformaldehyde-fixed HeLa cells using Arginase 1 polyclonal antibody (Product # PA5-85267) at a dilution of 1:200.

Arginase 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of Arginase 1 was performed in paraffin-embedded mouse liver tissue using Arginase 1 Polyclonal Antibody (Product # PA5-85267) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Arginase 1 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry (Frozen) analysis of Arginase-1 was performed in frozen-sectioned mouse hippocampus tissue using Arginase 1 Polyclonal Antibody (Product # PA5-85267) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI.

Arginase 1 Antibody in Western Blot (WB) — Knockout of Arginase 1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRIPSR760199_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Arginase 1 was performed by loading 30 µg of Hep G2 Cas9 (Lane 1) and Hep G2 Arginase 1 KO (Lane 2) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-Arginase 1 Polyclonal Antibody (Product # PA5-85267, 1:2000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:5000 dilution) using the iBright™ FL 1500 (Product # A44115). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to Arginase 1.

Arginase 1 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of Arginase 1 in HepG2 cells (control: unlabeled cells) using Arginase 1 polyclonal antibody (Product # PA5-85267) at a dilution of 1:50. Dylight 488-conjugated was used as a secondary antibody.

Arginase 1 Antibody in Immunoprecipitation (IP) — Immunoprecipitation analysis of Arginase 1 in HepG2 whole cell extracts with Arginase 1 polyclonal antibody (Product # PA5-85267) using 5 µg of sample, followed by anti-Rabbit IgG secondary antibody.

Arginase 1 Antibody in Immunohistochemistry (IHC) — Figure 6 CLU expression is associated with decreased ratio of NOS 2 + (M1 marker)/ARG 1 + (M2 marker) cells at day 7 following renal IRI. ARG 1 + and NOS 2 + cells were identified by immunohistochemistry as described in the ""Methods"" section. (a) The numbers of ARG 1 + cells. * P = 0.0010 (WT versus KO, two-tailed t -test). (b) The numbers of NOS 2 + cells. ** P = 0.0021 (WT versus KO, two-tailed t -test). (c) The difference in the M1/M2 ratio between the WT and CLU-KO groups was confirmed by the NOS 2 + /ARG 1 + ratio in each kidney. *** P = 0.0179 (WT versus KO, two-tailed t -test). Data in a , b and c are representative of three independent experiments and presented as the mean +- s.d. ( n = 3). (d) Representative images of three independent experiments. ARG 1 + (top 2) and NOS 2 + (bottom 2) cells (dark brown) in the tubulointerstitial area, the perivascular space and injured tubular epithelium of the renal cortical and medullary junction. Red arrowheads represent positively stained cells; Black arrowheads correspond to false-positive staining. Scale bar: 100 um. ARG 1; arginase 1; CLU, clusterin; DT, distal convoluted tubule; G, glomerulus; IA, interlobular artery; KO, clusterin knockout; NOS 2, nitric oxide synthase 2; PT, proximal convoluted tubule; WT, wild type.

Arginase 1 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein inArginase 1 KO cell line compared to control cell line using Anti-Arginase 1 Polyclonal Antibody (Product # PA5-85267). {KO}

Product Details

PA5-85267

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunohistochemistry (Frozen) (IHC (F))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Flow Cytometry (Flow)	1:50-1:200	View 1 publication 1 publication
ELISA (ELISA)	1:1,000-1:10,000	-
Immunoprecipitation (IP)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Full length human Arginase 1 Recombinant protein. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.53 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2792410

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (86%), Rat (86%), Pig (89%), Rabbit (91%), Bovine (90%).

Positive Control: Huh7, HepG2, mouse liver, rat liver.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

HA-coated collagen nanofibers for urethral regeneration via in situ polarization of M2 macrophages.

Journal of nanobiotechnology

Niu Y,Stadler FJ,Yang X,Deng F,Liu G,Xia H

Published figure using Arginase 1 polyclonal antibody (Product # PA5-85267) in Western Blot

Wed Sep 22 00:00:00 EDT 2021

DA-DRD5 signaling controls colitis by regulating colonic M1/M2 macrophage polarization.

Cell death & disease

Liu L,Wu Y,Wang B,Jiang Y,Lin L,Li X,Yang S

Published figure using Arginase 1 polyclonal antibody (Product # PA5-85267) in Flow Cytometry

Mon May 17 00:00:00 EDT 2021

Clusterin regulates macrophage expansion, polarization and phagocytic activity in response to inflammation in the kidneys.

Immunology and cell biology

Weng X,Zhao H,Guan Q,Shi G,Feng S,Gleave ME,Nguan CC,Du C

PA5-85267 was used in Immunohistochemistry to investigate the role of clusterin in macrophage polarization and phagocytosis.

Mon Mar 01 00:00:00 EST 2021

Clusterin regulates macrophage expansion, polarization and phagocytic activity in response to inflammation in the kidneys.

Immunology and cell biology

Weng X,Zhao H,Guan Q,Shi G,Feng S,Gleave ME,Nguan CC,Du C

PA5-85267 was used in Immunohistochemistry to investigate the role of clusterin in macrophage polarization and phagocytosis.

Mon Mar 01 00:00:00 EST 2021

Bioinformatics

Protein Aliases: A-I; AI type I arginase; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase

Gene Aliases: AI; AI256583; Arg-1; ARG1; PGIF

UniProt ID: (Human) P05089, (Rat) P07824, (Mouse) Q61176

Entrez Gene ID: (Human) 383, (Rat) 29221, (Mouse) 11846

Molecular Function: hydrolase

Function(s)

urea cycle liver development positive regulation of endothelial cell proliferation arginine catabolic process aging response to herbicide response to manganese ion response to zinc ion response to selenium ion regulation of L-arginine import response to amine arginine catabolic process to ornithine lung development collagen biosynthetic process response to vitamin A response to vitamin E response to drug response to amino acid response to cadmium ion response to axon injury response to methylmercury mammary gland involution maternal process involved in female pregnancy protein homotrimerization cellular response to hydrogen peroxide cellular response to lipopolysaccharide cellular response to interleukin-4 cellular response to glucagon stimulus cellular response to dexamethasone stimulus cellular response to transforming growth factor beta stimulus arginine metabolic process female pregnancy response to wounding response to lipopolysaccharide response to peptide hormone response to steroid hormone

Process(es)

arginase activity manganese ion binding hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines metal ion binding

Arginase 1 Polyclonal Antibody

Product Details

PA5-85267

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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