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Invitrogen
Arginase 1 Polyclonal Antibody
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FIGURE: 1 / 25
Arginase 1 Antibody (PA5-85267) in ICC/IF
Immunocytochemistry analysis of Arginase 1 in HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min. Samples were incubated with Arginase 1 polyclonal antibody (Product # PA5-85267) using a dilution of 1:500 (green). Blue: Fluoroshield with DAPI. Scale bar = 10 μm.
Product Details
PA5-85267
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length human Arginase 1 Recombinant protein.
Conjugate
Form
Liquid
Concentration
0.72 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (86%), Rat (86%), Pig (89%), Rabbit (91%), Bovine (90%).
Positive Control: Huh7, HepG2, mouse liver, rat liver.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: A-I; AI type I arginase; Arginase; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase
Gene Aliases: AI; AI256583; Arg-1; ARG1; PGIF
UniProt ID: (Human) P05089, (Rat) P07824, (Mouse) Q61176
Entrez Gene ID: (Human) 383, (Rat) 29221, (Mouse) 11846
urea cycle
liver development
positive regulation of endothelial cell proliferation
arginine catabolic process
aging
response to herbicide
response to manganese ion
response to zinc ion
response to selenium ion
regulation of L-arginine import
response to amine
arginine catabolic process to ornithine
lung development
collagen biosynthetic process
response to vitamin A
response to vitamin E
response to drug
response to amino acid
response to cadmium ion
response to axon injury
response to methylmercury
mammary gland involution
maternal process involved in female pregnancy
protein homotrimerization
cellular response to hydrogen peroxide
cellular response to lipopolysaccharide
cellular response to interleukin-4
cellular response to glucagon stimulus
cellular response to dexamethasone stimulus
cellular response to transforming growth factor beta stimulus
arginine metabolic process
female pregnancy
response to wounding
response to lipopolysaccharide
response to peptide hormone
response to steroid hormone
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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