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          • Primary Antibodies ›
          • At2g21960 Antibodies

          Agrisera

          At2g21960 Polyclonal Antibody

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          Cite At2g21960 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          At2g21960 Antibody in Western Blot (WB)
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          At2g21960 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

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          At2g21960 Antibody (AS15-2834) in WB

          2,4,8 µg of chlorophyll from Arabidopsis thaliana thylakoids were separated on 12% SDS-PAGE and blotted 1 h to nitrocellulose membrane using tank transfer. Blots were blocked with 10% Milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1,000 overnight with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed three times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit Ig G horseradish pe... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          At2g21960 Antibody in Western Blot (WB)

          Product Details

          AS15-2834

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          -
          Product Specifications

          Species Reactivity

          Plant

          Host/Isotype

          Rabbit

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          KLH-conjugated peptide, derived from Arabidopsis thaliana.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Amount

          50 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4

          Contains

          no preservative

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

          For reconstitution add 50 µL of sterile water.

          Specific Species Reactivity: Arabidopsis thaliana

          Target Information

          At2g21960 encodes a nuclear protein-coding gene product that localizes to the chloroplast thylakoid membrane, where it behaves as an integral membrane protein detectable in thylakoid preparations (apparent molecular mass around 35 kDa by immunoblot). The encoded polypeptide is predicted to be hydrophobic and membrane embedded, consistent with a role within the thylakoid membrane system rather than the soluble stroma or lumen. Functional annotation for At2g21960 remains limited, with major databases still classifying it as uncharacterized; however, its experimentally supported thylakoid association supports involvement in chloroplast/thylakoid membrane organization or photosynthetic membrane biogenesis/maintenance processes, potentially as a lineage-specific auxiliary factor required for proper assembly, stability, or regulation of thylakoid protein complexes.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: T26J12.20; T26J12_20

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          It has to be done as per old AB suggested Products section.

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