|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Western Blot (WB)||1 ug/ml|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Immunohistochemistry (IHC)||See 4 publications below|
|Western Blot (WB)||See 4 publications below|
|Immunohistochemistry - Free Floating (IHC (Free))||See 1 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Fruit fly, Human|
|Published species reactivity||Rat , Fruit fly , Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: M(1) S E R A A D D V R G E P R R A A(17) C|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-749 detects ataxin 7 protein from transfected cell lysate.
PA1-749 has successfully been used in immunohistochemistry and Western blot procedures. By Western blot, this antibody detects an ~96 kDa protein representing human ataxin 7 in transfected COS-1 cells.
The PA1-749 immunizing peptide corresponds to amino acid residues 1-17 from human ataxin 7. This sequence is completely conserved in mouse ataxin 7. This peptide (Cat. # PEP-217) is available for use in neutralization and control experiments.
Spinocerebellar ataxia type 7 (SCA7) belongs to a class of hereditary neurodegenrative diseases characterized by abnormally expanded polyglutamine (CAG) repeats at the N-terminus. This autosomal dominant cerebellar ataxia primarily affects the cerebellum, retina, and brain stem and causes dementia, macular degeneration and other neurodegenerative characteristics.
SCA7 gene encodes the ubiquitous 892 residue protein ataxin-7. This protein is typically located in the cytoplasm and on the nuclear membrane of normal brain neurons. In cells where there is a mutation of the SCA7 gene, ataxin 7 accumulates in intranuclear inclusions and can result in cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ADCAII; Ataxin-7; OPCA3; SCA7; Spinocerebellar ataxia type 7 protein
Gene Aliases: ADCAII; ATXN7; OPCA3; SCA7
UniProt ID: (Human) O15265
Entrez Gene ID: (Human) 6314
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