Invitrogen

Ataxin 7 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

18 Published Figures

23 References

View all (7) Ataxin 7 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Ataxin 7 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 22

Ataxin 7 Antibody (PA1-749) in ICC/IF

Immunofluorescence analysis of Ataxin 7 was performed using 70% confluent log phase U-87 MG cells treated with 100 ng of Nocodazole for 16 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ataxin 7 Rabbit Polyclonal Antibody (Product # PA1-749) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Seco... View More

Ataxin 7 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Ataxin 7 Antibody in Immunohistochemistry (Frozen) (IHC (F))

Ataxin 7 Antibody in Immunohistochemistry (IHC)

Ataxin 7 Antibody in Immunohistochemistry - Free Floating (IHC (Free))

Ataxin 7 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

PA1-749

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 2 publications 2 publications

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 3 publications 3 publications

Immunoprecipitation (IP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Fruit fly, Human

Published species

Fruit fly, Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: M(1) S E R A A D D V R G E P R R A A(17) C

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2061308

Product Specific Information

PA1-749 detects ataxin 7 protein from transfected cell lysate.

PA1-749 has successfully been used in immunohistochemistry and Western blot procedures. By Western blot, this antibody detects an ~96 kDa protein representing human ataxin 7 in transfected COS-1 cells.

The PA1-749 immunizing peptide corresponds to amino acid residues 1-17 from human ataxin 7. This sequence is completely conserved in mouse ataxin 7. This peptide (Cat. # PEP-217) is available for use in neutralization and control experiments.

Target Information

Spinocerebellar ataxia type 7 (SCA7) belongs to a class of hereditary neurodegenrative diseases characterized by abnormally expanded polyglutamine (CAG) repeats at the N-terminus. This autosomal dominant cerebellar ataxia primarily affects the cerebellum, retina, and brain stem and causes dementia, macular degeneration and other neurodegenerative characteristics. SCA7 gene encodes the ubiquitous 892 residue protein ataxin-7. This protein is typically located in the cytoplasm and on the nuclear membrane of normal brain neurons. In cells where there is a mutation of the SCA7 gene, ataxin 7 accumulates in intranuclear inclusions and can result in cell death.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SCA7 Mouse Cerebellar Pathology Reveals Preferential Downregulation of Key Purkinje Cell-Identity Genes and Shared Disease Signature with SCA1 and SCA2.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Niewiadomska-Cimicka A,Doussau F,Perot JB,Roux MJ,Keime C,Hache A,Piguet F,Novati A,Weber C,Yalcin B,Meziane H,Champy MF,Grandgirard E,Karam A,Messaddeq N,Eisenmann A,Brouillet E,Nguyen HHP,Flament J,Isope P,Trottier Y

PA1-749 was used in Western Blot to reveal a disease signature shared among several SCAs and uncovers potential molecular targets for their treatment.

Wed Jun 02 00:00:00 EDT 2021

DNAzyme Cleavage of CAG Repeat RNA in Polyglutamine Diseases.

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics

Zhang N,Bewick B,Schultz J,Tiwari A,Krencik R,Zhang A,Adachi K,Xia G,Yun K,Sarkar P,Ashizawa T

PA1-749 was used in Western Blot to develop a DNAzyme capable of destroying expanded CAG repeats present in polyglutamine diseases.

Thu Jul 01 00:00:00 EDT 2021

Respiratory dysfunction in a mouse model of spinocerebellar ataxia type 7.

Disease models & mechanisms

Fusco AF,Pucci LA,Switonski PM,Biswas DD,McCall AL,Kahn AF,Dhindsa JS,Strickland LM,La Spada AR,ElMallah MK

PA1-749 was used in Immunohistochemistry to reveal the importance of phrenic and hypoglossal motor neuron pathology associated with respiratory failure and upper airway dysfunction.

Thu Jul 01 00:00:00 EDT 2021

Metabolic and Organelle Morphology Defects in Mice and Human Patients Define Spinocerebellar Ataxia Type 7 as a Mitochondrial Disease.

Cell reports

Ward JM,Stoyas CA,Switonski PM,Ichou F,Fan W,Collins B,Wall CE,Adanyeguh I,Niu C,Sopher BL,Kinoshita C,Morrison RS,Durr A,Muotri AR,Evans RM,Mochel F,La Spada AR

Published figure using Ataxin 7 polyclonal antibody (Product # PA1-749) in Western Blot

Tue Jan 29 00:00:00 EST 2019

Mutant CAG Repeats Effectively Targeted by RNA Interference in SCA7 Cells.

Genes

Fiszer A,Wroblewska JP,Nowak BM,Krzyzosiak WJ

PA1749 was used in immunocytochemistry to suggest that the selective targeting of a repeat tract in a mutant ATXN7 transcript by RNA interference is a feasible approach to treat spinocerebellar ataxia type 7

Sat Dec 17 00:00:00 EST 2016

Lentiviral vector-mediated overexpression of mutant ataxin-7 recapitulates SCA7 pathology and promotes accumulation of the FUS/TLS and MBNL1 RNA-binding proteins.

Molecular neurodegeneration

Alves S,Marais T,Biferi MG,Furling D,Marinello M,El Hachimi K,Cartier N,Ruberg M,Stevanin G,Brice A,Barkats M,Sittler A

PA1-749 was used in immunohistochemistry - frozen section and western blot to study recapitulation of SCA7 pathology and promotion of accumulation of the FUS/TLS and MBNL1 RNA-binding proteins by lentiviral vector-mediated overexpression of mutant ataxin

Thu Jul 28 00:00:00 EDT 2016

Proteolytic cleavage of ataxin-7 promotes SCA7 retinal degeneration and neurological dysfunction.

Human molecular genetics

Guyenet SJ,Mookerjee SS,Lin A,Custer SK,Chen SF,Sopher BL,La Spada AR,Ellerby LM

PA1-749 was used in immunohistochemistry and western blot to determine if caspase cleavage contributes to spinocerebellar ataxia type 7 disease pathogenesis using transgenic mice.

Wed Jul 15 00:00:00 EDT 2015

Nonallele specific silencing of ataxin-7 improves disease phenotypes in a mouse model of SCA7.

Molecular therapy : the journal of the American Society of Gene Therapy

Ramachandran PS,Boudreau RL,Schaefer KA,La Spada AR,Davidson BL

PA1-749 was used in immunohistochemistry - free floating and western blot to test if reducing ataxin-7 in Purkinje cells is beneficial in an animal model of SCA7

Mon Sep 01 00:00:00 EDT 2014

Nonallele specific silencing of ataxin-7 improves disease phenotypes in a mouse model of SCA7.

Molecular therapy : the journal of the American Society of Gene Therapy

Ramachandran PS,Boudreau RL,Schaefer KA,La Spada AR,Davidson BL

PA1-749 was used in immunohistochemistry - free floating and western blot to test if reducing ataxin-7 in Purkinje cells is beneficial in an animal model of SCA7

Mon Sep 01 00:00:00 EDT 2014

RNA interference-based therapy for spinocerebellar ataxia type 7 retinal degeneration.

PloS one

Ramachandran PS,Bhattarai S,Singh P,Boudreau RL,Thompson S,Laspada AR,Drack AV,Davidson BL

PA1-749 was used in western blot to study the beneficial retinal effects of RNAi-mediated knockdown of ataxin-7 in spinocerebellar ataxia type 7

Fri Jan 09 00:00:00 EST 2015

Interferon β induces clearance of mutant ataxin 7 and improves locomotion in SCA7 knock-in mice.

Brain : a journal of neurology

Chort A,Alves S,Marinello M,Dufresnois B,Dornbierer JG,Tesson C,Latouche M,Baker DP,Barkats M,El Hachimi KH,Ruberg M,Janer A,Stevanin G,Brice A,Sittler A

PA1-749 was used in Western Blotting and Immunohistochemistry to show that interferon beta induces PML-dependent clearance of ataxin-7, and improves motor function.

Sat Jun 01 00:00:00 EDT 2013

Interferon β induces clearance of mutant ataxin 7 and improves locomotion in SCA7 knock-in mice.

Brain : a journal of neurology

Chort A,Alves S,Marinello M,Dufresnois B,Dornbierer JG,Tesson C,Latouche M,Baker DP,Barkats M,El Hachimi KH,Ruberg M,Janer A,Stevanin G,Brice A,Sittler A

PA1-749 was used in Western Blotting and Immunohistochemistry to show that interferon beta induces PML-dependent clearance of ataxin-7, and improves motor function.

Sat Jun 01 00:00:00 EDT 2013

Reduction of mutant ataxin-7 expression restores motor function and prevents cerebellar synaptic reorganization in a conditional mouse model of SCA7.

Human molecular genetics

Furrer SA,Waldherr SM,Mohanachandran MS,Baughn TD,Nguyen KT,Sopher BL,Damian VA,Garden GA,La Spada AR

PA1-749 was used in immunohistochemistry to study whether spinocerebellar ataxia type 7 neurological features and neurodegeneration are reversible in a conditional murine model

Fri Mar 01 00:00:00 EST 2013

Histone deacetylase-3 interacts with ataxin-7 and is altered in a spinocerebellar ataxia type 7 mouse model.

Molecular neurodegeneration

Duncan CE,An MC,Papanikolaou T,Rugani C,Vitelli C,Ellerby LM

Published figure using Ataxin 7 polyclonal antibody (Product # PA1-749) in Western Blot

Sun Oct 27 00:00:00 EDT 2013

Histone deacetylase-3 interacts with ataxin-7 and is altered in a spinocerebellar ataxia type 7 mouse model.

Molecular neurodegeneration

Duncan CE,An MC,Papanikolaou T,Rugani C,Vitelli C,Ellerby LM

Published figure using Ataxin 7 polyclonal antibody (Product # PA1-749) in Western Blot

Sun Oct 27 00:00:00 EDT 2013

Histone deacetylase-3 interacts with ataxin-7 and is altered in a spinocerebellar ataxia type 7 mouse model.

Molecular neurodegeneration

Duncan CE,An MC,Papanikolaou T,Rugani C,Vitelli C,Ellerby LM

Published figure using Ataxin 7 polyclonal antibody (Product # PA1-749) in Western Blot

Sun Oct 27 00:00:00 EDT 2013

Spinocerebellar ataxia type 7 cerebellar disease requires the coordinated action of mutant ataxin-7 in neurons and glia, and displays non-cell-autonomous bergmann glia degeneration.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Furrer SA,Mohanachandran MS,Waldherr SM,Chang C,Damian VA,Sopher BL,Garden GA,La Spada AR

PA1-749 was used in immunohistochemistry to investigate the role of ataxin-7 in spinocerebellar ataxia type 7 cerebellar disease

Wed Nov 09 00:00:00 EST 2011

Posttranslational modification of ataxin-7 at lysine 257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragment.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Mookerjee S,Papanikolaou T,Guyenet SJ,Sampath V,Lin A,Vitelli C,DeGiacomo F,Sopher BL,Chen SF,La Spada AR,Ellerby LM

PA1-749 was used in western blot and immunoprecipitation to study the effect of ataxin-7 post-translational modification on its autophagy-mediated proteolytic processing.

Wed Dec 02 00:00:00 EST 2009

Posttranslational modification of ataxin-7 at lysine 257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragment.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Mookerjee S,Papanikolaou T,Guyenet SJ,Sampath V,Lin A,Vitelli C,DeGiacomo F,Sopher BL,Chen SF,La Spada AR,Ellerby LM

PA1-749 was used in western blot and immunoprecipitation to study the effect of ataxin-7 post-translational modification on its autophagy-mediated proteolytic processing.

Wed Dec 02 00:00:00 EST 2009

A conditional pan-neuronal Drosophila model of spinocerebellar ataxia 7 with a reversible adult phenotype suitable for identifying modifier genes.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Latouche M,Lasbleiz C,Martin E,Monnier V,Debeir T,Mouatt-Prigent A,Muriel MP,Morel L,Ruberg M,Brice A,Stevanin G,Tricoire H

PA1-749 was used in immunohistochemistry to investigate a conditional Drosophila model for spinocerebellar ataxia 7 .

Wed Mar 07 00:00:00 EST 2007

Proteolytic cleavage of ataxin-7 by caspase-7 modulates cellular toxicity and transcriptional dysregulation.

The Journal of biological chemistry

Young JE,Gouw L,Propp S,Sopher BL,Taylor J,Lin A,Hermel E,Logvinova A,Chen SF,Chen S,Bredesen DE,Truant R,Ptacek LJ,La Spada AR,Ellerby LM

PA1-749 was used in western blot to study the relationship between ataxin-7 and caspase-7 and its role on SCA7 disease pathogenesis.

Fri Oct 12 00:00:00 EDT 2007

PML clastosomes prevent nuclear accumulation of mutant ataxin-7 and other polyglutamine proteins.

The Journal of cell biology

Janer A,Martin E,Muriel MP,Latouche M,Fujigasaki H,Ruberg M,Brice A,Trottier Y,Sittler A

PA1-749 was used in immunocytochemistry to study the pathogenesis of spinocerebellar ataxia type 7.

Mon Jul 03 00:00:00 EDT 2006

PML clastosomes prevent nuclear accumulation of mutant ataxin-7 and other polyglutamine proteins.

The Journal of cell biology

Janer A,Martin E,Muriel MP,Latouche M,Fujigasaki H,Ruberg M,Brice A,Trottier Y,Sittler A

PA1-749 was used in immunocytochemistry to study the pathogenesis of spinocerebellar ataxia type 7.

Mon Jul 03 00:00:00 EDT 2006

PML clastosomes prevent nuclear accumulation of mutant ataxin-7 and other polyglutamine proteins.

The Journal of cell biology

Janer A,Martin E,Muriel MP,Latouche M,Fujigasaki H,Ruberg M,Brice A,Trottier Y,Sittler A

PA1-749 was used in immunocytochemistry to study the pathogenesis of spinocerebellar ataxia type 7.

Mon Jul 03 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: Ataxin-7; Atxn7-PA; Atxn7-PB; Atxn7-PC; Atxn7-PD; CG9866-PA; CG9866-PB; CG9866-PC; CG9866-PD; LD40170p; Spinocerebellar ataxia type 7 protein

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Gene Aliases: ADCAII; Ataxin7; ATXN7; CG9866; Dmel\CG9866; Dmel_CG9866; OPCA3; SCA7

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UniProt ID: (Human) O15265

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Entrez Gene ID: (Human) 6314, (Fruit fly) 33423

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Function(s)

chromatin binding protein binding molecular_function

Process(es)

SAGA complex assembly positive regulation of histone H2B ubiquitination microtubule cytoskeleton organization transcription, DNA-templated nucleus organization visual perception histone deubiquitination negative regulation of phosphorylation negative regulation of insulin-like growth factor receptor signaling pathway positive regulation of transcription from RNA polymerase II promoter

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Ataxin 7 Polyclonal Antibody

Ataxin 7 Antibody (PA1-749) in ICC/IF

Product Details

PA1-749

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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