Immunofluorescence analysis of Aurora B Antibody was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Aurora B Antibody (365200) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa flour 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing Nuclear localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Bovine, Sheep, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the N-terminal region of the human Aurora B protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Aurora-B kinase (AurB) is a serine/threonine kinase which is the enzymatic core of the Chromosomal Passenger Complex (CPC). This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localization, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis: one containing all four members and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Aurora kinase inhibitors reveal mechanisms of HURP in nucleation of centrosomal and kinetochore microtubules.
36-5200 was used in western blot to identify and test Aurora kinases inhibitors for anti-tumor activity.
|Wu JM,Chen CT,Coumar MS,Lin WH,Chen ZJ,Hsu JT,Peng YH,Shiao HY,Lin WH,Chu CY,Wu JS,Lin CT,Chen CP,Hsueh CC,Chang KY,Kao LP,Huang CY,Chao YS,Wu SY,Hsieh HP,Chi YH||Proceedings of the National Academy of Sciences of the United States of America (110:E1779)||2013|
|Aurora B expression in post-puberal testicular germ cell tumours.||Esposito F,Libertini S,Franco R,Abagnale A,Marra L,Portella G,Chieffi P||Journal of cellular physiology (221:435)||2009|
|Not Applicable||Not Cited||
Aurora B expression correlates with aggressive behaviour in glioblastoma multiforme.
36-5200 was used in immunocytochemistry, immunohistochemistry - paraffin section, and western blot to elucidate the correlation between aggressive behaviour in glioblastoma multiforme and aurora B expression
|Zeng WF,Navaratne K,Prayson RA,Weil RJ||Journal of clinical pathology (60:218)||2007|