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Invitrogen
Aurora B Polyclonal Antibody
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FIGURE: 1 / 15
Aurora B Antibody (36-5200) in ICC/IF
Immunofluorescent analysis of Aurora B Antibody was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Aurora B Antibody (Product # 36-5200) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 45 minutes at room ... View More
Product Details
36-5200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Bovine,
Human,
Sheep
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the N-terminal region of the human Aurora B protein.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
The Aurora kinases associate with microtubules during chromosome movement and segregation. AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localization, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis: one containing all four members and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AIM-1; ARK-2; Aurora 1; Aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora- and IPL1-like midbody-associated protein 1; aurora-1; aurora-B; aurora-related kinase 2; Aurora/IPL1-related kinase 2; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase 12; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase aurora-B; STK-1
Gene Aliases: AIK2; AIM-1; AIM1; AIRK2; ARK2; AurB; AURKB; aurkb-sv1; aurkb-sv2; IPL1; PPP1R48; STK1; STK12; STK5
UniProt ID: (Human) Q96DV5
Entrez Gene ID: (Human) 9212
negative regulation of transcription from RNA polymerase II promoter
negative regulation of B cell apoptotic process
protein phosphorylation
spindle organization
sister chromatid cohesion
aging
cell proliferation
attachment of spindle microtubules to kinetochore
abscission
histone modification
protein sumoylation
anaphase-promoting complex-dependent catabolic process
spindle checkpoint
negative regulation of protein binding
positive regulation of telomere maintenance via telomerase
negative regulation of cytokinesis
positive regulation of cytokinesis
protein localization to kinetochore
cellular response to UV
cleavage furrow formation
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
histone H3-S28 phosphorylation
protein autophosphorylation
mitotic spindle midzone assembly
positive regulation of telomerase activity
regulation of chromosome segregation
regulation of signal transduction by p53 class mediator
positive regulation of telomere capping
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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