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Invitrogen
Aurora B Polyclonal Antibody
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FIGURE: 1 / 4
Aurora B Antibody (PA5-17632) in ICC/IF
Immunofluorescence analysis of Aurora B was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Aurora B Monoclonal Antibody (Product # PA5-17632) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # ... View More
Product Details
PA5-17632
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the amino terminus of human Aurora B/AIM1
Conjugate
Form
Liquid
Concentration
27 µg/mL
Amount
2.7 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
The Aurora kinases associate with microtubules during chromosome movement and segregation. AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localization, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis: one containing all four members and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AIM-1; ARK-2; Aurora 1; aurora B; Aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora- and IPL1-like midbody-associated protein 1; aurora- and Ipl1-like midbody-associated protein 1 homolog; aurora-1; aurora-B; aurora-related kinase 2; Aurora/IPL1-related kinase 2; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase 12; serine/threonine kinase 5; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase aurora-B; STK-1; unnamed protein product
Gene Aliases: AIK2; AIM-1; AIM1; AIRK2; AL022959; ARK-2; ARK2; AurB; AURKB; aurkb-sv1; aurkb-sv2; IPL1; PPP1R48; STK-1; STK1; STK12; STK5
UniProt ID: (Human) Q96GD4, (Mouse) O70126, (Rat) O55099
Entrez Gene ID: (Human) 9212, (Mouse) 20877, (Rat) 114592
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein serine/threonine/tyrosine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
kinase binding
protein serine kinase activity
transferase activity, transferring phosphorus-containing groups
histone serine kinase activity
metal ion binding
non-receptor serine/threonine protein kinase
negative regulation of transcription from RNA polymerase II promoter
mitotic cell cycle
mitotic cytokinesis
negative regulation of B cell apoptotic process
spindle organization
mitotic spindle organization
positive regulation of microtubule depolymerization
positive regulation of telomere maintenance
regulation of cytokinesis
negative regulation of cytokinesis
positive regulation of cytokinesis
regulation of microtubule-based process
protein localization to kinetochore
cellular response to UV
cleavage furrow formation
post-translational protein modification
cell cycle G2/M phase transition
mitotic cytokinesis checkpoint
negative regulation of innate immune response
mitotic spindle midzone assembly
cell division
attachment of mitotic spindle microtubules to kinetochore
regulation of chromosome segregation
midbody abscission
positive regulation of mitotic sister chromatid segregation
positive regulation of cell cycle process
positive regulation of mitotic cell cycle spindle assembly checkpoint
mitotic spindle assembly
repair of mitotic kinetochore microtubule attachment defect
cGAS/STING signaling pathway
negative regulation of cGAS/STING signaling pathway
regulation of signal transduction by p53 class mediator
positive regulation of mitotic sister chromatid separation
positive regulation of attachment of mitotic spindle microtubules to kinetochore
positive regulation of mitotic cytokinesis
positive regulation of lateral attachment of mitotic spindle microtubules to kinetochore
mitotic sister chromatid biorientation
protein phosphorylation
cell cycle
mitotic nuclear division
phosphorylation
spindle checkpoint
negative regulation of protein binding
histone H3-S28 phosphorylation
aging
cell proliferation
abscission
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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