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          • Primary Antibodies ›
          • B-Raf (K601E Mutant) Antibodies

          Invitrogen

          B-Raf (K601E Mutant) Polyclonal Antibody

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          Cite B-Raf (K601E Mutant) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          B-Raf (K601E Mutant) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          B-Raf (K601E Mutant) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          B-Raf (K601E Mutant) Antibody (PA5-143383) in ICC/IF

          Immunofluorescent analysis of B-Raf (K601E Mutant) in WT transfected and mutant transfected 293T cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: B-Raf (K601E Mutant) stained by B-Raf (K601E Mutant) polyclonal antibody (Product # PA5-143383) diluted at 1:500. Blue: Fluoroshield with DAPI. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          B-Raf (K601E Mutant) Antibody in Immunocytochemistry (ICC/IF)
          B-Raf (K601E Mutant) Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          PA5-143383

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          -

          Immunocytochemistry (ICC/IF)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Carrier-protein conjugated synthetic peptide surrounding mutant K601E of human B-Raf. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.54 mg/mL

          Amount

          54 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7, with 1% BSA, 20% glycerol

          Contains

          0.025% Proclin 300

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2938378

          Product Specific Information

          Store as concentrated solution.

          Centrifuge briefly prior to opening vial.

          Target Information

          This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases|This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion|Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung|Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes|A pseudogene of this gene has been identified on the X chromosome

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); B-raf; B-Raf proto-oncogene, serine/threonine kinase; B-RAF1; BRAF1; carboxypeptidase A1 (pancreatic); casein kinase I alpha S-like; caspase 1, apoptosis-related cysteine peptidase; CKI-alpha; CKI-alpha-like; class IVb beta tubulin; clock regulator kinase; CSNK1A1; cysteine protease; DNA ADP-ribosyltransferase PARP1; down-regulated in lung cancer; epididymis secretory sperm binding protein; epididymis secretory sperm binding protein Li 77p; G protein beta1/gamma2 subunit-interacting factor 1; H3 histone family, member A; HIST1H3A; histone 1, H3a; histone cluster 1 H3 family member a; histone cluster 1, H3a; histone H3/a; IL-1 beta-converting enzyme; IL1B-convertase; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; microtubule protein; microtubule-associated protein tau; NAD(+) ADP-ribosyltransferase 1; neurofibrillary tangle protein; NS7; paired helical filament-tau; pancreatic carboxypeptidase A; PHF-tau; poly (ADP-ribose) polymerase family, member 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; poly[ADP-ribose] synthase 1; protein phosphatase 1, regulatory subunit 103; protein poly-ADP-ribosyltransferase PARP1; RAFB1; Tau-derived paired helical filament hexapeptide; tubulin beta-2 chain; tubulin beta-2C chain; tubulin, beta 2C; tubulin, beta, 2; unnamed protein product

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          Gene Aliases: ADPRT; ADPRT 1; ADPRT1; ARTD1; Beta2; CK1; CK1a; CKIa; CPA; DDPAC; FTD1; FTDP-17; H3/A; H3FA; HEL-S-77p; HIST1H3A; HLCDGP1; ICE; IL1BC; LCAEOD; MAPTL; MSTD; MTBT1; MTBT2; P45; pADPRT-1; PARP; PARP-1; PARS; Poly-PARP; PPND; PPOL; PPP1R103; PRO2975; TAU; tau-40; Tau-PHF6; TUBB2; TUBB2C

          View more View less

          UniProt ID: (Human) P29466, (Human) P15085, (Human) P48729, (Human) Q8N752, (Human) P68431, (Human) P10636, (Human) P09874, (Human) P68371

          View more View less

          Entrez Gene ID: (Human) 834, (Human) 1357, (Human) 1452, (Human) 122011, (Human) 8350, (Human) 4137, (Human) 142, (Human) 10383

          View more View less

          Function(s)
          endopeptidase activity cysteine-type endopeptidase activity protein binding cysteine-type peptidase activity cysteine-type endopeptidase activator activity involved in apoptotic process enzyme binding kinase binding cytokine binding identical protein binding CARD domain binding metallocarboxypeptidase activity exopeptidase activity zinc ion binding protein kinase activity protein serine/threonine kinase activity ATP binding protein serine kinase activity DNA binding structural constituent of chromatin nucleosomal DNA binding cadherin binding protein heterodimerization activity AT DNA binding double-stranded DNA binding single-stranded DNA binding RNA binding actin binding enzyme inhibitor activity microtubule binding tubulin binding SH3 domain binding protein kinase binding protein binding, bridging apolipoprotein binding dynactin binding phosphatidylinositol binding sequence-specific DNA binding chaperone binding protein phosphatase 2A binding Hsp90 protein binding lipoprotein particle binding histone-dependent DNA binding microtubule lateral binding microtubule stabilizing activity phosphatidylinositol bisphosphate binding chromatin binding damaged DNA binding NAD+ ADP-ribosyltransferase activity enzyme activator activity estrogen receptor binding nucleosome binding ubiquitin protein ligase binding protein homodimerization activity histone deacetylase binding non-sequence-specific DNA binding, bending NAD binding RNA polymerase II sequence-specific DNA binding transcription factor binding R-SMAD binding NAD DNA ADP-ribosyltransferase activity transcription regulator activator activity NAD+-protein-serine ADP-ribosyltransferase activity NAD+-protein-aspartate ADP-ribosyltransferase activity NAD+-protein-glutamate ADP-ribosyltransferase activity NAD+-protein-tyrosine ADP-ribosyltransferase activity NAD+-protein-histidine ADP-ribosyltransferase activity NAD+-histone H2BS6 serine ADP-ribosyltransferase activity NAD+-histone H3S10 serine ADP-ribosyltransferase activity NAD+-histone H2BE35 glutamate ADP-ribosyltransferase activity single-strand break-containing DNA binding protein ADP-ribosylase activity double-stranded RNA binding GTPase activity structural constituent of cytoskeleton GTP binding MHC class I protein binding
          Process(es)
          microtubule cytoskeleton organization mitotic cell cycle cytoskeleton organization microtubule-based process sperm motility natural killer cell mediated cytotoxicity pattern recognition receptor signaling pathway proteolysis apoptotic process inflammatory response signal transduction osmosensory signaling pathway protein processing protein autoprocessing response to lipopolysaccharide positive regulation of interleukin-1 beta production positive regulation of interleukin-18 production defense response to bacterium regulation of apoptotic process positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of neuron apoptotic process icosanoid biosynthetic process regulation of inflammatory response positive regulation of inflammatory response protein maturation defense response to virus pyroptosis cellular response to lipopolysaccharide cellular response to mechanical stimulus cellular response to interferon-gamma positive regulation of release of cytochrome c from mitochondria cytokine precursor processing signaling receptor ligand precursor processing AIM2 inflammasome complex assembly positive regulation of NLRP3 inflammasome complex assembly positive regulation of tumor necrosis factor-mediated signaling pathway microglial cell activation cellular response to DNA damage stimulus cell-cell signaling synapse assembly learning or memory memory response to lead ion regulation of autophagy negative regulation of gene expression negative regulation of mitochondrial membrane potential rRNA metabolic process axonal transport of mitochondrion central nervous system neuron development regulation of microtubule polymerization or depolymerization regulation of microtubule polymerization positive regulation of microtubule polymerization cytoplasmic microtubule organization neuron projection development positive regulation of superoxide anion generation regulation of chromosome organization stress granule assembly cellular response to heat cellular response to reactive oxygen species positive regulation of axon extension microtubule polymerization astrocyte activation regulation of synaptic plasticity intracellular distribution of mitochondria generation of neurons synapse organization regulation of calcium-mediated signaling protein polymerization protein homooligomerization regulation of microtubule-based movement axon development regulation of microtubule cytoskeleton organization plus-end-directed organelle transport along microtubule regulation of mitochondrial fission negative regulation of mitochondrial fission fibril organization axonal transport regulation of cellular response to heat regulation of long term synaptic depression positive regulation of protein localization to synapse neurofibrillary tangle assembly negative regulation of establishment of protein localization to mitochondrion positive regulation of cellular protein localization amyloid fibril formation cellular response to nerve growth factor stimulus cellular response to brain-derived neurotrophic factor stimulus single strand break repair negative regulation of transcription from RNA polymerase II promoter telomere maintenance DNA repair double-strand break repair transcription from RNA polymerase II promoter mitochondrion organization transforming growth factor beta receptor signaling pathway response to gamma radiation positive regulation of cardiac muscle hypertrophy carbohydrate biosynthetic process signal transduction involved in regulation of gene expression macrophage differentiation DNA ADP-ribosylation mitochondrial DNA metabolic process positive regulation of DNA-templated transcription, elongation cellular response to insulin stimulus regulation of protein localization positive regulation of intracellular estrogen receptor signaling pathway negative regulation of transcription elongation from RNA polymerase II promoter cellular response to oxidative stress cellular response to UV protein modification process mitochondrial DNA repair regulation of circadian sleep/wake cycle, non-REM sleep response to ethanol negative regulation of innate immune response negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter decidualization positive regulation of mitochondrial depolarization positive regulation of SMAD protein import into nucleus positive regulation of necroptotic process protein poly-ADP-ribosylation protein auto-ADP-ribosylation protein localization to chromatin establishment of protein localization to chromatin cellular response to zinc ion cellular response to transforming growth factor beta stimulus replication fork reversal negative regulation of cGAS/STING signaling pathway positive regulation of protein localization to nucleus cellular response to oxygen-containing compound regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway response to aldosterone negative regulation of adipose tissue development negative regulation of telomere maintenance via telomere lengthening cellular response to beta-amyloid positive regulation of myofibroblast differentiation regulation of base-excision repair positive regulation of double-strand break repair via homologous recombination ATP generation from poly-ADP-D-ribose negative regulation of ATP biosynthetic process protein phosphorylation Golgi organization cell surface receptor signaling pathway Wnt signaling pathway viral protein processing regulation of Wnt signaling pathway cellular response to nutrient positive regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of Rho protein signal transduction proteasome-mediated ubiquitin-dependent protein catabolic process intermediate filament cytoskeleton organization negative regulation of canonical Wnt signaling pathway negative regulation of NLRP3 inflammasome complex assembly positive regulation of TORC1 signaling leukotriene metabolic process protein catabolic process response to cadmium ion chromatin organization nucleosome assembly heterochromatin assembly telomere organization regulation of gene expression, epigenetic
          It has to be done as per old AB suggested Products section.

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