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Invitrogen
B-Raf (K601E Mutant) Polyclonal Antibody
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FIGURE: 1 / 2
B-Raf (K601E Mutant) Antibody (PA5-143383) in ICC/IF
Immunofluorescent analysis of B-Raf (K601E Mutant) in WT transfected and mutant transfected 293T cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: B-Raf (K601E Mutant) stained by B-Raf (K601E Mutant) polyclonal antibody (Product # PA5-143383) diluted at 1:500. Blue: Fluoroshield with DAPI.
Product Details
PA5-143383
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide surrounding mutant K601E of human B-Raf. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.54 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% Proclin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Store as concentrated solution.
Centrifuge briefly prior to opening vial.
Target Information
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases|This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion|Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung|Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes|A pseudogene of this gene has been identified on the X chromosome
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-raf; B-Raf proto-oncogene, serine/threonine kinase; B-RAF1; BRAF1; casein kinase 1, alpha 1; casein kinase 1, alpha 1-like; casein kinase I alpha S-like; Casein kinase I isoform alpha; Casein kinase I isoform alpha-like; CASP-1; CASP1 nirs variant 1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); Caspase-1; CK1; CKI-alpha; CKI-alpha-like; clock regulator kinase; down-regulated in lung cancer; epididymis secretory sperm binding protein Li 77p; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1B-convertase; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; NS7; p45; RAFB1
Gene Aliases: CASP1; CK1; CK1a; CKIa; CSNK1A1; CSNK1A1L; HEL-S-77p; HLCDGP1; ICE; IL1BC; IL1BCE; P45; PRO2975
UniProt ID: (Human) P29466, (Human) P48729, (Human) Q8N752
Entrez Gene ID: (Human) 834, (Human) 1452, (Human) 122011
endopeptidase activity
cysteine-type endopeptidase activity
protein binding
cysteine-type endopeptidase activator activity involved in apoptotic process
cysteine-type endopeptidase activity involved in apoptotic process
protein kinase activity
protein serine/threonine kinase activity
ATP binding
kinase activity
protease
non-receptor serine/threonine protein kinase
response to hypoxia
proteolysis
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
signal transduction
regulation of autophagy
protein processing
response to lipopolysaccharide
interleukin-1 beta production
response to ATP
regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of interleukin-1 alpha secretion
positive regulation of interleukin-1 beta secretion
regulation of inflammatory response
mitochondrial depolarization
membrane hyperpolarization
pyroptosis
cellular response to mechanical stimulus
cellular response to organic substance
programmed necrotic cell death
toxin transport
protein phosphorylation
phagocytosis
Golgi organization
mitotic nuclear division
cell surface receptor signaling pathway
regulation of cell shape
Wnt signaling pathway
peptidyl-serine phosphorylation
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
intermediate filament cytoskeleton organization
cell division
negative regulation of canonical Wnt signaling pathway
regulation of GTP binding
beta-catenin destruction complex assembly
beta-catenin destruction complex disassembly
cell morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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