Search

  • Order Status
  • Quick Order

Invitrogen

B-Raf (K601E Mutant) Polyclonal Antibody

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Datasheet
Certificates
Protocols
Questions & Answers
Tech Support
Additional Information:
{{banner.description}}
{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}

Product Details

PA5-143383

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide surrounding mutant K601E of human B-Raf. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.54 mg/mL

Amount

54 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2938378

Product Specific Information

Store as concentrated solution.

Centrifuge briefly prior to opening vial.

Target Information

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases|This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion|Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung|Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes|A pseudogene of this gene has been identified on the X chromosome

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: B-raf; B-Raf proto-oncogene, serine/threonine kinase; B-RAF1; BRAF1; casein kinase I alpha S-like; Casein kinase I isoform alpha; Casein kinase I isoform alpha-like; CASP-1; caspase 1, apoptosis-related cysteine peptidase; Caspase-1; CK1; CKI-alpha; CKI-alpha-like; clock regulator kinase; CSNK1A1; cysteine protease; down-regulated in lung cancer; epididymis secretory sperm binding protein Li 77p; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1B-convertase; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; NS7; p45; RAFB1; unnamed protein product

View more View less

Gene Aliases: CASP1; CK1; CK1a; CKIa; CSNK1A1; CSNK1A1L; HEL-S-77p; HLCDGP1; ICE; IL1BC; IL1BCE; P45; PRO2975

View more View less

UniProt ID: (Human) P29466, (Human) P48729, (Human) Q8N752

View more View less

Entrez Gene ID: (Human) 834, (Human) 1452, (Human) 122011

View more View less

Function(s)
endopeptidase activity cysteine-type endopeptidase activity protein binding peptidase activity cysteine-type peptidase activity cysteine-type endopeptidase activator activity involved in apoptotic process hydrolase activity enzyme binding kinase binding cytokine binding identical protein binding CARD domain binding nucleotide binding protein kinase activity protein serine/threonine kinase activity ATP binding kinase activity transferase activity protein serine kinase activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter plasma membrane repair pattern recognition receptor signaling pathway proteolysis apoptotic process inflammatory response signal transduction osmosensory signaling pathway protein secretion protein autoprocessing protein catabolic process response to lipopolysaccharide positive regulation of interleukin-1 beta production positive regulation of interleukin-18 production interleukin-18-mediated signaling pathway defense response to bacterium regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling icosanoid biosynthetic process ceramide biosynthetic process regulation of inflammatory response positive regulation of inflammatory response protein maturation defense response to virus protein poly-ADP-ribosylation pyroptosis interleukin-1-mediated signaling pathway cellular response to lipopolysaccharide cellular response to mechanical stimulus cellular response to interferon-gamma cytokine precursor processing signaling receptor ligand precursor processing AIM2 inflammasome complex assembly pyroptotic cell death positive regulation of tumor necrosis factor-mediated signaling pathway autophagosome assembly protein phosphorylation Golgi organization cell surface receptor signaling pathway negative regulation of autophagy positive regulation of autophagy Wnt signaling pathway viral protein processing regulation of Wnt signaling pathway SCF-dependent proteasomal ubiquitin-dependent protein catabolic process cellular response to nutrient levels cellular response to nutrient positive regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of Rho protein signal transduction TORC1 signaling proteasome-mediated ubiquitin-dependent protein catabolic process NLRP3 inflammasome complex assembly intermediate filament cytoskeleton organization cell division negative regulation of canonical Wnt signaling pathway negative regulation of NLRP3 inflammasome complex assembly positive regulation of NLRP3 inflammasome complex assembly negative regulation of TORC1 signaling positive regulation of TORC1 signaling
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support