Invitrogen

B-Raf (V600E) Recombinant Rabbit Monoclonal Antibody (RM8)

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B-Raf (V600E) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of formalin fixed and paraffin embedded Thyroid Carcinoma tissue. Allele specific PCR validated to be Positive for BRAF V600E.

B-Raf (V600E) Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of formalin fixed and paraffin embedded melanoma tissue sections. Samples were probed using a B-Raf Antibody (Product # MA5-24661).

B-Raf (V600E) Antibody in Western Blot (WB) — Western Blot analysis of cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein. Samples were probed using a B-Raf Antibody (Product # MA5-24661).

B-Raf (V600E) Antibody in ELISA (ELISA) — Sandwich ELISA performed on the lysates of 293T cells overexpressing BRAF V600E or BRAF wild type His-tag proteins, shows the specificity of RM8 to the V600E mutation. Samples were probed using a B-Raf Antibody (Product # MA5-24661) and His-tag fusion proteins were captured by an Anti-His-Tag antibody.

Product Details

MA5-24661

Applications	Tested Dilution	Publications
Western Blot (WB)	0.5-2 µg/mL	View 1 publication 1 publication
Immunohistochemistry (IHC)	2-10 µg/mL	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	2-10 µg/mL	-
ELISA (ELISA)	0.5-2 µg/mL	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	RM8
Immunogen	Peptide corresponding to B-Raf V600E mutant.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.2-7.4, with 50% glycerol, 1% BSA
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2661889

Product Specific Information

This antibody reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

B-raf belongs to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in the gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

LGR5⁺ epithelial tumor stem-like cells generate a 3D-organoid model for ameloblastoma.

Cell death & disease

Chang TH,Shanti RM,Liang Y,Zeng J,Shi S,Alawi F,Carrasco L,Zhang Q,Le AD

MA5-24661 was used in Immunohistochemistry to explore whether LGR5+ epithelial cells in Ameloblastoma (AM) possess stem-like cell properties and their potential contribution to pathogenesis and recurrence of AM.

Thu May 07 00:00:00 EDT 2020

Src-Dependent DBL Family Members Drive Resistance to Vemurafenib in Human Melanoma.

Cancer research

Feddersen CR,Schillo JL,Varzavand A,Vaughn HR,Wadsworth LS,Voigt AP,Zhu EY,Jennings BM,Mullen SA,Bobera J,Riordan JD,Stipp CS,Dupuy AJ

MA5-24661 was used in Western Blotting to show a simple, rapid, and flexible genetic screening approach that identifies genes that drive resistance to MAPK inhibitors when overexpressed in human melanoma cells.

Tue Oct 01 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); FLJ95109; MGC126806; murine sarcoma viral (v-raf) oncogene homolog B1; p94; Proto-oncogene B-Raf; Serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B; v-Raf murine sarcoma viral oncogene homolog B1

Gene Aliases: B-raf; B-RAF1; BRAF; BRAF1; NS7; RAFB1

UniProt ID: (Human) P15056

Entrez Gene ID: (Human) 673

Molecular Function: non-receptor serine/threonine protein kinase