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Invitrogen

B-Raf (V600E) Recombinant Rabbit Monoclonal Antibody (RM8), Biotin

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B-Raf (V600E) Recombinant Rabbit Monoclonal Antibody (RM8), Biotin

Product Details

MA5-44555

Applications
Tested Dilution
Publications

Western Blot (WB)

0.5-2 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-5 µg/mL
-

Immunocytochemistry (ICC/IF)

0.5-5 µg/mL
-

Flow Cytometry (Flow)

Assay-dependent
-

ELISA (ELISA)

0.5-2 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

RM8

Immunogen

A peptide corresponding to BRAF V600E mutant. The immunogen range is a synthetic peptide within amino acids 550-650 of Akt with V600E mutant.
3D Epitope / Immunogen

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

1 mg/mL

Amount

50 µg

Purification

Protein A

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2926685

Product Specific Information

This antibody reacts to the Histone H3.3 G34R mutant. No cross reactivity with wild type Histone H3.3.

Target Information

This gene, B-RAF/BRAF, encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-Raf serine/threonine-protein; murine sarcoma viral (v-raf) oncogene homolog B1; p94; Proto-oncogene B-Raf; serine/threonine protein kinase; Serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B; v-Raf murine sarcoma viral oncogene homolog B1

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Gene Aliases: B-raf; B-RAF1; BRAF; BRAF-1; BRAF1; NS7; RAFB1

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UniProt ID: (Human) P15056

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Entrez Gene ID: (Human) 673

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Function(s)
nucleotide binding catalytic activity protein kinase activity protein serine/threonine kinase activity MAP kinase kinase activity MAP kinase kinase kinase activity calcium ion binding protein binding ATP binding zinc ion binding kinase activity transferase activity small GTPase binding identical protein binding metal ion binding scaffold protein binding protein serine kinase activity
Process(es)
MAPK cascade myeloid progenitor cell differentiation protein phosphorylation signal transduction epidermal growth factor receptor signaling pathway visual learning organ morphogenesis positive regulation of gene expression negative regulation of fibroblast migration positive regulation of glucose transport synaptic vesicle exocytosis cell differentiation thyroid gland development T cell differentiation in thymus positive regulation of peptidyl-serine phosphorylation substrate adhesion-dependent cell spreading somatic stem cell population maintenance regulation of cell proliferation negative regulation of apoptotic process stress fiber assembly CD4-positive, alpha-beta T cell differentiation positive T cell selection CD4-positive or CD8-positive, alpha-beta T cell lineage commitment negative regulation of neuron apoptotic process regulation of T cell differentiation alpha-beta T cell differentiation thymus development regulation of axon regeneration positive regulation of axon regeneration positive regulation of axonogenesis T cell receptor signaling pathway positive regulation of stress fiber assembly long-term synaptic potentiation head morphogenesis face development ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion cellular response to xenobiotic stimulus endothelial cell apoptotic process establishment of protein localization to membrane postsynaptic modulation of chemical synaptic transmission positive regulation of substrate adhesion-dependent cell spreading negative regulation of synaptic vesicle exocytosis negative regulation of endothelial cell apoptotic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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