Invitrogen

B3GALTL Polyclonal Antibody

View all (3) B3GALTL antibodies

Questions & Answers

Questions & Answers

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B3GALTL Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of B3GALTL in HUVEC cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with B3GALTL polyclonal antibody (Product # PA5-101457) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

B3GALTL Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded B3GALTL in mouse testis tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with B3GALTL polyclonal antibody (Product # PA5-101457) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

B3GALTL Antibody in Western Blot (WB) — Western blot analysis of B3GALTL in HepG2 cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with B3GALTL polyclonal antibody (Product # PA5-101457).

Product Details

PA5-101457

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human B3GALTL, corresponding to a region within C-terminal amino acids. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2850892

Target Information

The protein encoded by this gene is a beta-1, 3-glucosyltransferase that transfers glucose to O-linked fucosylglycans on thrombospondin type-1 repeats (TSRs) of several proteins. The encoded protein is a type II membrane protein. Defects in this gene are a cause of Peters-plus syndrome (PPS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: beta 1,3-galactosyltransferase-like; Beta 3-glucosyltransferase; beta 3-glycosyltransferase-like; Beta-1,3-glucosyltransferase; Beta-3-glycosyltransferase-like; Beta3Glc-T; UDP-GAL:beta-GlcNAc beta-1,3-galactosyltransferase-like

Gene Aliases: B3GALTL; B3Glc-T; B3GLCT; B3GTL; beta3Glc-T; Gal-T; Gm1057

UniProt ID: (Human) Q6Y288, (Mouse) Q8BHT6

Entrez Gene ID: (Human) 145173, (Mouse) 381694

Molecular Function: glycosyltransferase

fucose metabolic process protein O-linked fucosylation carbohydrate metabolic process biological_process

Process(es)

transferase activity, transferring glycosyl groups alpha-1,6-mannosyltransferase activity alpha-1,2-mannosyltransferase activity mannosyltransferase activity alpha-1,3-mannosyltransferase activity alpha-1,3-galactosyltransferase activity molecular_function UDP-glucose:glycoprotein glucosyltransferase activity glycolipid mannosyltransferase activity oligosaccharyl transferase activity dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity acetylglucosaminyltransferase activity acetylgalactosaminyltransferase activity galactosyltransferase activity fucosyltransferase activity O antigen polymerase activity lipopolysaccharide-1,6-galactosyltransferase activity transferase activity cellulose synthase activity 9-phenanthrol UDP-glucuronosyltransferase activity 1-phenanthrol glycosyltransferase activity 9-phenanthrol glycosyltransferase activity 1,2-dihydroxy-phenanthrene glycosyltransferase activity phenanthrol glycosyltransferase activity beta-1,4-mannosyltransferase activity alpha-1,2-galactosyltransferase activity dolichyl pyrophosphate Man7GlcNAc2 alpha-1,3-glucosyltransferase activity lipopolysaccharide-1,5-galactosyltransferase activity dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity inositol phosphoceramide synthase activity glucosyltransferase activity alpha-(1->3)-fucosyltransferase activity alpha-(1->6)-fucosyltransferase activity indole-3-butyrate beta-glucosyltransferase activity salicylic acid glucosyltransferase (ester-forming) activity salicylic acid glucosyltransferase (glucoside-forming) activity benzoic acid glucosyltransferase activity chondroitin hydrolase activity dolichyl-pyrophosphate Man7GlcNAc2 alpha-1,6-mannosyltransferase activity cytokinin 9-beta-glucosyltransferase activity