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Invitrogen
B3GNT5 Polyclonal Antibody
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FIGURE: 1 / 1
B3GNT5 Antibody (PA5-89820) in WB
Western blot analysis of B3GNT5 in extracts of various cell lines. Samples were incubated with B3GNT5 Polyclonal antibody (Product # PA5-89820) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-89820
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 36-378 of human B3GNT5 (NP_1144361)
Conjugate
Form
Liquid
Concentration
2.03 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: DNHIVSHMKS YSYRYLINSY DFVNDTLSLK HTSAGPRYQY LINHKEKCQA QDVLLLLFVK TAPENYDRRS GIRRTWGNEN YVRSQLNANI KTLFALGTPN PLEGEELQRK LAWEDQRYND IIQQDFVDSF YNLTLKLLMQ FSWANTYCPH AKFLMTADDD IFIHMPNLIE YLQSLEQIGV QDFWIGRVHR GAPPIRDKSS KYYVSYEMYQ WPAYPDYTAG AAYVISGDVA AKVYEASQTL NSSLYIDDVF MGLCANKIGI VPQDHVFFSG EGKTPYHPCI YEKMMTSHGH LEDLQDLWKN ATDPKVKTIS KGFFGQIYCR LMKIILLCKI SYVDTYPCRA AFI; Positive Samples: HT-29, A-549; Cellular Location: Golgi apparatus membrane, Single-pass type II membrane protein
Target Information
B3GNT5 is a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II membrane protein. It exhibits strong activity to transfer GlcNAc to glycolipid substrates and is identified as the most likely candidate for lactotriaosylceramide synthase. This enzyme is essential for the expression of Lewis X epitopes on glycolipids.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: beta 1,3 N-acetyglucosaminyltransferase Lc3 synthase; beta-1,3-Gn-T5; beta-1,3-N-acetylglucosaminyltransferase 5; beta-1,3-N-acetylglucosaminyltransferase bGnT-5; beta3GnT5; glycosyltransferase; BGnT-5; Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase; Lactotriaosylceramide synthase; Lc(3)Cer synthase; lc3 synthase; UDP-GlcNAc:beta-Gal beta-1,3-N-acetylglucosaminyltransferase 5; unnamed protein product
Gene Aliases: B3GN-T5; B3GNT5; beta3Gn-T5
UniProt ID: (Human) Q9BYG0
Entrez Gene ID: (Human) 84002
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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