Invitrogen

BACE1 Polyclonal Antibody

28 Published Figures

27 References

View all (14) BACE1 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

BACE1 Antibody — Published figure using BACE1 polyclonal antibody (Product # PA1-757)

BACE1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of BACE1 (green) showing staining in the Golgi apparatus of Neuro-2a cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a BACE1 polyclonal antibody (Product # PA1-757) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

BACE1 Antibody in Western Blot (WB) — Western blot analysis of BACE1 was performed by loading 20 µg of SH-SY5Y whole cell lysates and 10 µL PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk/TBST for at least 1 hour at room temperature. The membrane was probed with a BACE1 polyclonal antibody (Product # PA1-757, “-“) or BACE1 polyclonal antibody pre-incubated with immunizing peptide (Product # PEP-093, “+”) at a dilution of 1:1000 overnight at 4°C on a rocking platform, followed by a goat anti-rabbit IgG-HRP Superclonal secondary antibody (Product # A27036) at a dilution of 1:1,000 for at least 1 hour at room temperature. Detection was performed using SuperSignal West Dura (Product # 34076).

Product Details

Tested Applications

Dilution

ELISA (ELISA)

Assay dependent

Immunocytochemistry (ICC)

1:20-1:200

Immunofluorescence (IF)

1:20-1:200

Immunoprecipitation (IP)

Assay dependent

Western Blot (WB)

1:200-1:2000

Published Applications

Immunocytochemistry (ICC)

See 1 publication below

Immunofluorescence (IF)

See 3 publications below

Western Blot (WB)

See 18 publications below

Immunoprecipitation (IP)

See 4 publications below

ELISA (ELISA)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C(485) L R Q Q H D D F A D D I S L L K(501) of human beta-secretase.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_325863

Product Specific Information

PA1-757 detects transfected beta-secretase 1.

PA1-757 has been successfully used in ELISA, ICC/IF, Western blot and immunoprecipitation applications. The molecular weight of BACE1 detected may vary depending on the lysate tested. This may be due to glycosylation differences in various cell lines.

The PA1-757 immunizing peptide (Cat. # PEP-093) is available for use in neutralization and control experiments.

Target Information

Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer's disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE), of which there are two isoforms, BACE1 and 2. This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. Found associated with the presenilins is the transmembrane glycoprotein nicastrin. Nicastrin has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: APP beta-secretase; asp 2; ASP2; Aspartyl protease 2; BACE1; Beta-secretase 1; beta-secretase 1 precursor variant 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; Beta-Site APP-Cleaving Enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2

Gene Aliases: ASP2; BACE; BACE1; C76936; HSPC104; KIAA1149

UniProt ID: (Human) P56817, (Mouse) P56818

Entrez Gene ID: (Human) 23621, (Mouse) 23821

Molecular Function: aspartic protease hydrolase protease