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Immunofluorescent analysis of BACE1 (green) showing staining in the Golgi apparatus of Neuro-2a cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a BACE1 polyclonal antibody (Product # PA1-757) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C(485) L R Q Q H D D F A D D I S L L K(501) of human beta-secretase.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:200-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-757 detects transfected beta-secretase 1.
PA1-757 has been successfully used in ELISA, ICC/IF, Western blot and immunoprecipitation applications. The molecular weight of BACE1 detected may vary depending on the lysate tested. This may be due to glycosylation differences in various cell lines.
The PA1-757 immunizing peptide (Cat. # PEP-093) is available for use in neutralization and control experiments.
Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer"e;s disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE), of which there are two isoforms, BACE1 and 2. This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. Found associated with the presenilins is the transmembrane glycoprotein nicastrin. Nicastrin has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Elucidation of the BACE1 regulating factor GGA3 in Alzheimer's disease.
PA1-757 was used in western blot to study whether the BACE1-modulator GGA3 contributes to Alzheimer's disease pathology
|Natunen T,Parrado AR,Helisalmi S,Pursiheimo JP,Sarajärvi T,Mäkinen P,Kurkinen KM,Mullin K,Alafuzoff I,Haapasalo A,Bertram L,Soininen H,Tanzi RE,Hiltunen M||Journal of Alzheimer's disease : JAD (37:217)||2013|
Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport.
PA1-757 was used in western blot to study the role of calsyntenin-1 in protecting APP from processing by ectodomain proteases during anterograde axonal transport
|Steuble M,Diep TM,Schätzle P,Ludwig A,Tagaya M,Kunz B,Sonderegger P||Biology open (1:761)||2012|
Depletion of GGA1 and GGA3 mediates postinjury elevation of BACE1.
PA1-757 was used in western blot to study the role of GGA1 and GGA3 in the elevation of BACE1 in the acute phase after injury
|Walker KR,Kang EL,Whalen MJ,Shen Y,Tesco G||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:10423)||2012|
Involvement of receptor tyrosine kinase Tyro3 in amyloidogenic APP processing and β-amyloid deposition in Alzheimer's disease models.
PA1-757 was used in western blot to study the role of receptor tyrosine kinase Tyro3 in APP processing and beta amyloid deposition
|Zheng Y,Wang Q,Xiao B,Lu Q,Wang Y,Wang X||PloS one (7:null)||2012|
Minocycline corrects early, pre-plaque neuroinflammation and inhibits BACE-1 in a transgenic model of Alzheimer's disease-like amyloid pathology.
PA1-757 was used in western blot to study the effect of minocycline on neuroinflammation and BACE-1 activity in a transgenic model of Alzheimer's disease
|Ferretti MT,Allard S,Partridge V,Ducatenzeiler A,Cuello AC||Journal of neuroinflammation (9:null)||2012|
The contribution of activated astrocytes to Aβ production: implications for Alzheimer's disease pathogenesis.
PA1-757 was used in western blot to investigate the involvement of activated astrocytes in beta-amyloid production in Alzheimer disease
|Zhao J,O'Connor T,Vassar R||Journal of neuroinflammation (8:null)||2011|
Docosahexaenoic acid-derived neuroprotectin D1 induces neuronal survival via secretase- and PPARγ-mediated mechanisms in Alzheimer's disease models.
PA1-757 was used in western blot to study the mechanism for the effect of docosahexaenoic acid-derived neuroprotectin D1 on neuronal survival
|Zhao Y,Calon F,Julien C,Winkler JW,Petasis NA,Lukiw WJ,Bazan NG||PloS one (6:null)||2011|
Down-regulation of seladin-1 increases BACE1 levels and activity through enhanced GGA3 depletion during apoptosis.
PA1-757 was used in western blot to investigate how seladin-1 regulates BACE1 levels and activity during apoptosis
|Sarajärvi T,Haapasalo A,Viswanathan J,Mäkinen P,Laitinen M,Soininen H,Hiltunen M||The Journal of biological chemistry (284:34433)||2009|
Thiamine deficiency induces oxidative stress and exacerbates the plaque pathology in Alzheimer's mouse model.
PA1-757 was used in western blot to investigate the effect of thiamine deficiency on Alzheimer's pathogenesis
|Karuppagounder SS,Xu H,Shi Q,Chen LH,Pedrini S,Pechman D,Baker H,Beal MF,Gandy SE,Gibson GE||Neurobiology of aging (30:1587)||2009|
Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion.
PA1-757 was used in western blot to investigate the role of ubiquilin 1 during the amyloid precursor protein trafficking and Abeta secretion.
|Hiltunen M,Lu A,Thomas AV,Romano DM,Kim M,Jones PB,Xie Z,Kounnas MZ,Wagner SL,Berezovska O,Hyman BT,Tesco G,Bertram L,Tanzi RE||The Journal of biological chemistry (281:32240)||2006|
Energy inhibition elevates beta-secretase levels and activity and is potentially amyloidogenic in APP transgenic mice: possible early events in Alzheimer's disease pathogenesis.
PA1-757 was used in western blot to investigate the role of impaired energy production in the brain in the AD pathogenesis.
|Velliquette RA,O'Connor T,Vassar R||The Journal of neuroscience : the official journal of the Society for Neuroscience (25:10874)||2005|
BACE is degraded via the lysosomal pathway.
PA1-757 was used in western blot to investigate BACE degradation via the lysosomal pathway.
|Koh YH,von Arnim CA,Hyman BT,Tanzi RE,Tesco G||The Journal of biological chemistry (280:32499)||2005|
Statins cause intracellular accumulation of amyloid precursor protein, beta-secretase-cleaved fragments, and amyloid beta-peptide via an isoprenoid-dependent mechanism.
PA1-757 was used in western blot to demonstrate the effect of isoprenylation on intracellular amyloid beta-peptide level.
|Cole SL,Grudzien A,Manhart IO,Kelly BL,Oakley H,Vassar R||The Journal of biological chemistry (280:18755)||2005|
Presenilin 1 stabilizes the C-terminal fragment of the amyloid precursor protein independently of gamma-secretase activity.
PA1-757 was used in western blot to demonstrate the effect of presenilin 1 on Abeta production .
|Pitsi D,Octave JN||The Journal of biological chemistry (279:25333)||2004|
Ubiquitin regulates GGA3-mediated degradation of BACE1.
PA1-757 was used in immunoprecipitation to investigate the effect of ubiquitin during GGA3-mediated degradation of BACE1
|Kang EL,Cameron AN,Piazza F,Walker KR,Tesco G||The Journal of biological chemistry (285:24108)||2010|
Phosphorylation of the translation initiation factor eIF2alpha increases BACE1 levels and promotes amyloidogenesis.
PA1-757 was used in immunoprecipitation to study the roles of the eIF2alpha phosphorylation in sporadic AD
|O'Connor T,Sadleir KR,Maus E,Velliquette RA,Zhao J,Cole SL,Eimer WA,Hitt B,Bembinster LA,Lammich S,Lichtenthaler SF,Hébert SS,De Strooper B,Haass C,Bennett DA,Vassar R||Neuron (60:988)||2008|
The metabolism of beta-amyloid converting enzyme and beta-amyloid precursor protein processing.
PA1-757 was used in immunoprecipitation to investigate BACE metabolism and its influence on beta-amyloid production
|Benjannet S,Cromlish JA,Diallo K,Chrétien M,Seidah NG||Biochemical and biophysical research communications (325:235)||2004|
Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE.
PA1-757 was used in immunoprecipitation to investigate beta-secretase cleavage of APP protein mediated by BACE
|Vassar R,Bennett BD,Babu-Khan S,Kahn S,Mendiaz EA,Denis P,Teplow DB,Ross S,Amarante P,Loeloff R,Luo Y,Fisher S,Fuller J,Edenson S,Lile J,Jarosinski MA,Biere AL,Curran E,Burgess T,Louis JC,Collins F,Treanor J,Rogers G,Citron M||Science (New York, N.Y.) (286:735)||1999|
Beta-site amyloid precursor protein cleaving enzyme 1 levels become elevated in neurons around amyloid plaques: implications for Alzheimer's disease pathogenesis.
PA1-757 was used in ELISA to study the expression of beta-site amyloid precursor protein cleaving enzyme 1 in neurons neighboring amyloid plaques.
|Zhao J,Fu Y,Yasvoina M,Shao P,Hitt B,O'Connor T,Logan S,Maus E,Citron M,Berry R,Binder L,Vassar R||The Journal of neuroscience : the official journal of the Society for Neuroscience (27:3639)||2007|
APP beta-secretase; asp 2; ASP2; aspartyl protease 2; BACE1; beta-secretase 1; beta-secretase 1 precursor variant 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; beta-site amyloid precursor protein cleaving enzyme 1; beta-site APP cleaving enzyme 1; Beta-Site APP-Cleaving Enzyme 1; memapsin-2; membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2
ASP2; BACE; BACE1; C76936; HSPC104; KIAA1149