Invitrogen

BACE1 Polyclonal Antibody

31 Published Figures

29 References

View all (16) BACE1 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

BACE1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of BACE1 (green) showing staining in the Golgi apparatus of Neuro-2a cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a BACE1 polyclonal antibody (Product # PA1-757) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

BACE1 Antibody in Western Blot (WB) — Western blot analysis of BACE1 was performed by loading 20 µg of SH-SY5Y whole cell lysates and 10 µL PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk/TBST for at least 1 hour at room temperature. The membrane was probed with a BACE1 polyclonal antibody (Product # PA1-757, “-“) or BACE1 polyclonal antibody pre-incubated with immunizing peptide (Product # PEP-093, “+”) at a dilution of 1:1000 overnight at 4°C on a rocking platform, followed by a goat anti-rabbit IgG-HRP Superclonal secondary antibody (Product # A27036) at a dilution of 1:1,000 for at least 1 hour at room temperature. Detection was performed using SuperSignal West Dura (Product # 34076).

BACE1 Antibody — Published figure using BACE1 polyclonal antibody (Product # PA1-757)

Product Details

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay dependent

View 1 publication 1 publication

Immunocytochemistry (ICC)

1:20-1:200

View 2 publications 2 publications

Immunofluorescence (IF)

1:20-1:200

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay dependent

View 4 publications 4 publications

Western Blot (WB)

1:200-1:2000

View 19 publications 19 publications

Product Specifications

Species

Human, Mouse

Published species

Artificial Control, Human, Mouse

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C(485) L R Q Q H D D F A D D I S L L K(501) of human beta-secretase.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_325863

Product Specific Information

PA1-757 detects transfected beta-secretase 1.

PA1-757 has been successfully used in ELISA, ICC/IF, Western blot and immunoprecipitation applications. The molecular weight of BACE1 detected may vary depending on the lysate tested. This may be due to glycosylation differences in various cell lines.

The PA1-757 immunizing peptide (Cat. # PEP-093) is available for use in neutralization and control experiments.

Target Information

Accumulation of the amyloid-b (Ab) plaque in the cerebral cortex is a critical event in the pathogenesis Alzheimer's disease. Ab peptide is generated by proteolytic cleavage of the b-amyloid protein precursor(APP) at b- and g-sites by two proteases. APP is first cleaved by b-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-b peptide, which is deposited in the brains of all sufferers of Alzheimer's disease. The long-sought b-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). bACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: APP beta-secretase; asp 2; ASP2; Aspartyl protease 2; BACE 1; BACE-1D; BACE-I-432; Beta secretase; Beta-secretase 1; beta-secretase 1 precursor variant 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; beta-site APP-cleaving enzyme 1; Memapsin; Memapsin-2; Memapsin2; Membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2

Gene Aliases: ASP2; BACE; BACE1; C76936; HSPC104; KIAA1149

UniProt ID: (Human) P56817, (Mouse) P56818

Entrez Gene ID: (Human) 23621, (Mouse) 23821

Molecular Function: aspartic protease hydrolase protease