Invitrogen

BACE1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

45 Published Figures

39 References

View all (19) BACE1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

FIGURE: 1 / 51

BACE1 Antibody (PA1-757) in WB

Western blot analysis of BACE1 was performed by loading 25 µg of U87-MG (Lane 1), Hela (Lane 2) and mouse brain (Lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a BACE1 polyclonal antibody (Product # PA1-757) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blo... View More

Product Image Gallery

BACE1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of BACE1 (green) showing staining in the Golgi apparatus of Neuro-2a cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a BACE1 polyclonal antibody (Product # PA1-757) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

BACE1 Antibody in Western Blot, Immunoprecipitation (WB, IP) — Figure 1. The beta-secretase BACE1 interacts with gamma-secretase at endogenous levels in WT mouse brain microsomal lysate. (A) 1% CHAPSO-solubilized lysates of WT mouse brain microsomes were immunoprecipitated for BACE1 or ITGalpha1, ITGalpha2, and ITGbeta1 as controls. IPs were blotted to probe for coIP of the gamma-components NCT and PS1-NTF. (B) 1% CHAPSO-solubilized lysates of WT mouse brain microsomes were loaded onto BN-PAGE followed by second-dimension SDS-PAGE and blotted with antibodies to the indicated proteins. *, nonspecific signal; m and im, mature and immature forms.

BACE1 Antibody in Immunoprecipitation (IP) — Figure 2. BACE1 exists with gamma-secretase in an HMW complex isolated from mouse brain by FPLC and has proteolytic activity. (A) 1% CHAPSO-solubilized WT mouse brain microsomes were fractionated on a Superose 6 Increase SEC column by FPLC and blotted with antibodies to the indicated proteins. (B) FPLC fractions were pooled into HMW (6-9) or LMW (21-24) fractions and immunoprecipitated for BACE1 and then blotted with antibodies to the indicated proteins. Note that the amount of BACE1 that was immunoprecipitated varies and may not necessarily reflect the amount in the input. (C) FPLC fractions were blotted with certain antibodies to APP (top two panels, C7; bottom panel, 1G6). (D) FPLC fractions were incubated with a fluorogenic BACE1 peptide substrate. After incubation for 2 h at 37degC, fluorescence was read as BACE1 activity ( n = 3; error bars are SD). (E) 0.25% CHAPSO-solubilized WT mouse brain microsomes were immunoprecipitated for gamma-secretase (anti-NCT) in the absence or presence of two different beta-secretase inhibitors (AZD3293 and inhibitor IV, each at 10 uM), or else anti-TfR as a control. beta-Secretase activity assays were performed on the eluates extracted from the IP resin. n = 5 for the NCT IP, n = 2 for the TfR IP; error bars are SD. Unpaired Student's t test: **, P < 0.01; ***, P < 0.001. (F) Pooled HMW and LMW FPLC fractions from WT mouse brains were immunoprecipitated for gamma-secretase (anti-NCT). beta-Secretase activity assays were performed on the

BACE1 Antibody in ELISA (ELISA) — Published figure using BACE1 polyclonal antibody (Product # PA1-757) in ELISA

BACE1 Antibody — Figure 2 Combinations of pro-inflammatory cytokines elevate endogenous BACE1 levels in mouse primary astrocyte cultures . (A, B) Cell lysates of cytokine-stimulated mouse primary astrocytes analyzed in Fig. 1A-C were analyzed for BACE1 by immunoblot. (A) BACE1 immunoblot images. The mature BACE1 band at 70 kDa is indicated by the arrowhead. Pro-inflammatory agents (alone and combinations) and stimulation times are indicated. Cell lysate from un-stimulated BACE1 -/- primary astrocytes was used as a negative control, while cell lysate from a stable BACE1-overexpressing HEK-293 cell line was used as a positive control. GFAP and beta-actin immunosignals were loading controls as in Fig 1A-C. (B) Histograms represent quantifications of BACE1 immunoblot signals in (A) expressed as percent of vehicle control. Note that cytokine combinations including TNF-alpha and IFN-gamma were generally more potent at increasing endogenous BACE1 levels in astrocytes over time in culture, raising BACE1 levels to ~600% of control. (C) mRNAs prepared from cytokine-stimulated primary astrocytes in Fig 1D were analyzed for endogenous BACE1 mRNA levels by TaqMan quantitative RT-PCR. Histograms represent quantifications of BACE1 mRNA levels expressed as percent of vehicle control. Note that astrocytic BACE1 mRNA levels were significantly reduced by TNF-alpha+IFN-gamma stimulation (C), even though BACE1 protein levels were increased several fold in similarly treated astrocytes (B). Statistical analysis was {TM}

Product Details

PA1-757

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:2,000

View 28 publications 28 publications

Immunocytochemistry (ICC/IF)

1:20-1:200

View 5 publications 5 publications

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C(485) L R Q Q H D D F A D D I S L L K(501) of human beta-secretase.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_325863

Product Specific Information

PA1-757 detects transfected beta-secretase 1.

PA1-757 has been successfully used in ELISA, ICC/IF, Western blot and immunoprecipitation applications. The molecular weight of BACE1 detected may vary depending on the lysate tested. This may be due to glycosylation differences in various cell lines.

The PA1-757 immunizing peptide (Cat. # PEP-093) is available for use in neutralization and control experiments.

Target Information

BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides (Abeta) found in extracellular amyloid plaques of Alzheimer's disease (AD). In some cases early onset familial AD can be attributed to a "Swedish" mutation in the amyloid precursor protein (APP), which dramatically enhances the cleavage of this protein by BACE1. This and other genetic and pathological evidence has led to therapeutic approaches that have focused on the inhibition of BACE1 and other APP-cleaving enzymes, such as gamma-secretase stuff. APP is first cleaved by b-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-b peptide, which is deposited in the brains of all sufferers of Alzheimer's disease. The long-sought b-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). bACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Gut-derived β-amyloid: Likely a centerpiece of the gut-brain axis contributing to Alzheimer's pathogenesis.

Gut microbes

Jin J,Xu Z,Zhang L,Zhang C,Zhao X,Mao Y,Zhang H,Liang X,Wu J,Yang Y,Zhang J

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Western Blot

Tue Jan 24 00:00:00 EST 2023

Activation of the hypoxia response protects mice from amyloid-β accumulation.

Cellular and molecular life sciences : CMLS

Ollonen T,Kurkela M,Laitakari A,Sakko S,Koivisto H,Myllyharju J,Tanila H,Serpi R,Koivunen P

PA1-757 was used in Western Blotting to suggest that non-full-scale activation of the HIF pathway appears to mediate protection against neurodegeneration via several mechanisms and should be studied as a treatment option for AD.

Tue Jul 19 00:00:00 EDT 2022

Alzheimer's disease protease-containing plasma extracellular vesicles transfer to the hippocampus via the choroid plexus.

EBioMedicine

Lee JH,Ostalecki C,Oberstein T,Schierer S,Zinser E,Eberhardt M,Blume K,Plosnita B,Stich L,Bruns H,Coras R,Vera-Gonzales J,Maler M,Baur AS

PA1-757 was used in Western Blot to conclude that protease-containing pEV could be part of a communication axis between the periphery and the brain that could be become detrimental depending on pEV concentration and duration of target cell impact.

Tue Mar 01 00:00:00 EST 2022

The novel anti-colitic effect of β-adrenergic receptors via modulation of PS1/BACE-1/Aβ axis and NOTCH signaling in an ulcerative colitis model.

Frontiers in pharmacology

Nasser S,Abdallah DM,Ahmed KA,Abdel-Mottaleb Y,El-Abhar HS

PA1-757 was used in Western Blotting to investigate the impact of mirabegron (β3-agonist) and/or carvedilol (β1/β2 antagonist) on iodoacetamide-induced ulcerative colitis with emphasis on the novel pathomechanism of the PS1/BACE-1/Aβ axis in ulcerative colitis, and its relation to the inflammatory cascade, fibrotic processes, and the gut barrier dysfunction.

Fri Nov 18 00:00:00 EST 2022

Amyloidogenic Processing of Amyloid Precursor Protein Drives Stretch-Induced Disruption of Axonal Transport in hiPSC-Derived Neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Chaves RS,Tran M,Holder AR,Balcer AM,Dickey AM,Roberts EA,Bober BG,Gutierrez E,Head BP,Groisman A,Goldstein LSB,Almenar-Queralt A,Shah SB

PA1-757 was used in Immunocytochemistry to reveal potential subcellular pathways contributing to the development of AD-associated phenotypes following mild traumatic brain injury, and putative strategies for intervening in these pathways.

Wed Dec 08 00:00:00 EST 2021

Protein farnesylation is upregulated in Alzheimer's human brains and neuron-specific suppression of farnesyltransferase mitigates pathogenic processes in Alzheimer's model mice.

Acta neuropathologica communications

Jeong A,Cheng S,Zhong R,Bennett DA,Bergö MO,Li L

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Western Blot

Tue Jul 27 00:00:00 EDT 2021

Protein farnesylation is upregulated in Alzheimer's human brains and neuron-specific suppression of farnesyltransferase mitigates pathogenic processes in Alzheimer's model mice.

Acta neuropathologica communications

Jeong A,Cheng S,Zhong R,Bennett DA,Bergö MO,Li L

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Western Blot

Tue Jul 27 00:00:00 EDT 2021

Upregulation of APP endocytosis by neuronal aging drives amyloid-dependent synapse loss.

Journal of cell science

Burrinha T,Martinsson I,Gomes R,Terrasso AP,Gouras GK,Almeida CG

PA1-757 was used in Western Blot to identify APP endocytosis upregulation as a potential mechanism of neuronal aging and, thus, a novel target to prevent late-onset Alzheimer's disease.

Sat May 01 00:00:00 EDT 2021

A cellular complex of BACE1 and γ-secretase sequentially generates Aβ from its full-length precursor.

The Journal of cell biology

Liu L,Ding L,Rovere M,Wolfe MS,Selkoe DJ

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Immunoprecipitation

Mon Feb 04 00:00:00 EST 2019

A cellular complex of BACE1 and γ-secretase sequentially generates Aβ from its full-length precursor.

The Journal of cell biology

Liu L,Ding L,Rovere M,Wolfe MS,Selkoe DJ

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Immunoprecipitation

Mon Feb 04 00:00:00 EST 2019

Bin1 and CD2AP polarise the endocytic generation of beta-amyloid.

EMBO reports

Ubelmann F,Burrinha T,Salavessa L,Gomes R,Ferreira C,Moreno N,Guimas Almeida C

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Immunofluorescence

Sun Jan 01 00:00:00 EST 2017

Bin1 and CD2AP polarise the endocytic generation of beta-amyloid.

EMBO reports

Ubelmann F,Burrinha T,Salavessa L,Gomes R,Ferreira C,Moreno N,Guimas Almeida C

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Immunofluorescence

Sun Jan 01 00:00:00 EST 2017

Bin1 and CD2AP polarise the endocytic generation of beta-amyloid.

EMBO reports

Ubelmann F,Burrinha T,Salavessa L,Gomes R,Ferreira C,Moreno N,Guimas Almeida C

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Immunofluorescence

Sun Jan 01 00:00:00 EST 2017

Relationship between ubiquilin-1 and BACE1 in human Alzheimer's disease and APdE9 transgenic mouse brain and cell-based models.

Neurobiology of disease

Natunen T,Takalo M,Kemppainen S,Leskelä S,Marttinen M,Kurkinen KMA,Pursiheimo JP,Sarajärvi T,Viswanathan J,Gabbouj S,Solje E,Tahvanainen E,Pirttimäki T,Kurki M,Paananen J,Rauramaa T,Miettinen P,Mäkinen P,Leinonen V,Soininen H,Airenne K,Tanzi RE,Tanila H,Haapasalo A,Hiltunen M

PA1-757 was used in western blot to compare the relationship between BACE1 and ubiquilin-1 in human Alzheimer's disease, APdE9 transgenic mouse brain and cell-based models

Fri Jan 01 00:00:00 EST 2016

Cathepsin L Mediates the Degradation of Novel APP C-Terminal Fragments.

Biochemistry

Wang H,Sang N,Zhang C,Raghupathi R,Tanzi RE,Saunders A

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Western Blot

Tue May 12 00:00:00 EDT 2015

Cathepsin L Mediates the Degradation of Novel APP C-Terminal Fragments.

Biochemistry

Wang H,Sang N,Zhang C,Raghupathi R,Tanzi RE,Saunders A

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Western Blot

Tue May 12 00:00:00 EDT 2015

Elucidation of the BACE1 regulating factor GGA3 in Alzheimer's disease.

Journal of Alzheimer's disease : JAD

Natunen T,Parrado AR,Helisalmi S,Pursiheimo JP,Sarajärvi T,Mäkinen P,Kurkinen KM,Mullin K,Alafuzoff I,Haapasalo A,Bertram L,Soininen H,Tanzi RE,Hiltunen M

PA1-757 was used in western blot to study whether the BACE1-modulator GGA3 contributes to Alzheimer's disease pathology

Tue Apr 08 00:00:00 EDT 2014

Depletion of GGA1 and GGA3 mediates postinjury elevation of BACE1.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Walker KR,Kang EL,Whalen MJ,Shen Y,Tesco G

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Western Blot

Wed Jul 25 00:00:00 EDT 2012

Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport.

Biology open

Steuble M,Diep TM,Schätzle P,Ludwig A,Tagaya M,Kunz B,Sonderegger P

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Immunofluorescence

Wed Aug 15 00:00:00 EDT 2012

Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport.

Biology open

Steuble M,Diep TM,Schätzle P,Ludwig A,Tagaya M,Kunz B,Sonderegger P

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Immunofluorescence

Wed Aug 15 00:00:00 EDT 2012

Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport.

Biology open

Steuble M,Diep TM,Schätzle P,Ludwig A,Tagaya M,Kunz B,Sonderegger P

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Immunofluorescence

Wed Aug 15 00:00:00 EDT 2012

Minocycline corrects early, pre-plaque neuroinflammation and inhibits BACE-1 in a transgenic model of Alzheimer's disease-like amyloid pathology.

Journal of neuroinflammation

Ferretti MT,Allard S,Partridge V,Ducatenzeiler A,Cuello AC

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Western Blot

Mon Apr 02 00:00:00 EDT 2012

Involvement of receptor tyrosine kinase Tyro3 in amyloidogenic APP processing and β-amyloid deposition in Alzheimer's disease models.

PloS one

Zheng Y,Wang Q,Xiao B,Lu Q,Wang Y,Wang X

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Western Blot

Thu Nov 08 00:00:00 EST 2012

BACE1 protein endocytosis and trafficking are differentially regulated by ubiquitination at lysine 501 and the Di-leucine motif in the carboxyl terminus.

The Journal of biological chemistry

Kang EL,Biscaro B,Piazza F,Tesco G

PA1-757 was used in Immunocytochemistry to assess the effects of the lack of ubiquitination at Lys-501 in Β-Site amyloid precursor protein-cleaving enzyme.

Fri Dec 14 00:00:00 EST 2012

Development of a specific ELISA to measure BACE1 levels in human tissues.

Journal of neuroscience methods

Gonzales A,Decourt B,Walker A,Condjella R,Nural H,Sabbagh MN

PA1-757 was used in Western Blotting to develop an immunoassay capable of measuring BACE1 levels in human brain and platelet lysates, as a potential marker of Alzheimer's disease.

Sun Oct 30 00:00:00 EDT 2011

The contribution of activated astrocytes to Aβ production: implications for Alzheimer's disease pathogenesis.

Journal of neuroinflammation

Zhao J,O'Connor T,Vassar R

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Western Blot

Wed Nov 02 00:00:00 EDT 2011

The contribution of activated astrocytes to Aβ production: implications for Alzheimer's disease pathogenesis.

Journal of neuroinflammation

Zhao J,O'Connor T,Vassar R

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Western Blot

Wed Nov 02 00:00:00 EDT 2011

Docosahexaenoic acid-derived neuroprotectin D1 induces neuronal survival via secretase- and PPARγ-mediated mechanisms in Alzheimer's disease models.

PloS one

Zhao Y,Calon F,Julien C,Winkler JW,Petasis NA,Lukiw WJ,Bazan NG

PA1-757 was used in western blot to study the mechanism for the effect of docosahexaenoic acid-derived neuroprotectin D1 on neuronal survival

Wed Jan 05 00:00:00 EST 2011

Ubiquitin regulates GGA3-mediated degradation of BACE1.

The Journal of biological chemistry

Kang EL,Cameron AN,Piazza F,Walker KR,Tesco G

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Immunoprecipitation

Fri Jul 30 00:00:00 EDT 2010

Ubiquitin regulates GGA3-mediated degradation of BACE1.

The Journal of biological chemistry

Kang EL,Cameron AN,Piazza F,Walker KR,Tesco G

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Immunoprecipitation

Fri Jul 30 00:00:00 EDT 2010

Ubiquitin regulates GGA3-mediated degradation of BACE1.

The Journal of biological chemistry

Kang EL,Cameron AN,Piazza F,Walker KR,Tesco G

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Immunoprecipitation

Fri Jul 30 00:00:00 EDT 2010

Thiamine deficiency induces oxidative stress and exacerbates the plaque pathology in Alzheimer's mouse model.

Neurobiology of aging

Karuppagounder SS,Xu H,Shi Q,Chen LH,Pedrini S,Pechman D,Baker H,Beal MF,Gandy SE,Gibson GE

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Western Blot

Thu Oct 01 00:00:00 EDT 2009

Down-regulation of seladin-1 increases BACE1 levels and activity through enhanced GGA3 depletion during apoptosis.

The Journal of biological chemistry

Sarajärvi T,Haapasalo A,Viswanathan J,Mäkinen P,Laitinen M,Soininen H,Hiltunen M

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Immunofluorescence

Fri Dec 04 00:00:00 EST 2009

Down-regulation of seladin-1 increases BACE1 levels and activity through enhanced GGA3 depletion during apoptosis.

The Journal of biological chemistry

Sarajärvi T,Haapasalo A,Viswanathan J,Mäkinen P,Laitinen M,Soininen H,Hiltunen M

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Immunofluorescence

Fri Dec 04 00:00:00 EST 2009

Down-regulation of seladin-1 increases BACE1 levels and activity through enhanced GGA3 depletion during apoptosis.

The Journal of biological chemistry

Sarajärvi T,Haapasalo A,Viswanathan J,Mäkinen P,Laitinen M,Soininen H,Hiltunen M

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Immunofluorescence

Fri Dec 04 00:00:00 EST 2009

Phosphorylation of the translation initiation factor eIF2alpha increases BACE1 levels and promotes amyloidogenesis.

Neuron

O'Connor T,Sadleir KR,Maus E,Velliquette RA,Zhao J,Cole SL,Eimer WA,Hitt B,Bembinster LA,Lammich S,Lichtenthaler SF,Hébert SS,De Strooper B,Haass C,Bennett DA,Vassar R

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Western Blot

Fri Dec 26 00:00:00 EST 2008

Phosphorylation of the translation initiation factor eIF2alpha increases BACE1 levels and promotes amyloidogenesis.

Neuron

O'Connor T,Sadleir KR,Maus E,Velliquette RA,Zhao J,Cole SL,Eimer WA,Hitt B,Bembinster LA,Lammich S,Lichtenthaler SF,Hébert SS,De Strooper B,Haass C,Bennett DA,Vassar R

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Western Blot

Fri Dec 26 00:00:00 EST 2008

Beta-site amyloid precursor protein cleaving enzyme 1 levels become elevated in neurons around amyloid plaques: implications for Alzheimer's disease pathogenesis.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Zhao J,Fu Y,Yasvoina M,Shao P,Hitt B,O'Connor T,Logan S,Maus E,Citron M,Berry R,Binder L,Vassar R

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in ELISA

Wed Apr 04 00:00:00 EDT 2007

Beta-site amyloid precursor protein cleaving enzyme 1 levels become elevated in neurons around amyloid plaques: implications for Alzheimer's disease pathogenesis.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Zhao J,Fu Y,Yasvoina M,Shao P,Hitt B,O'Connor T,Logan S,Maus E,Citron M,Berry R,Binder L,Vassar R

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in ELISA

Wed Apr 04 00:00:00 EDT 2007

Temporal memory deficits in Alzheimer's mouse models: rescue by genetic deletion of BACE1.

The European journal of neuroscience

Ohno M,Chang L,Tseng W,Oakley H,Citron M,Klein WL,Vassar R,Disterhoft JF

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Western Blot

Sun Jan 01 00:00:00 EST 2006

Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion.

The Journal of biological chemistry

Hiltunen M,Lu A,Thomas AV,Romano DM,Kim M,Jones PB,Xie Z,Kounnas MZ,Wagner SL,Berezovska O,Hyman BT,Tesco G,Bertram L,Tanzi RE

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Western Blot

Fri Oct 27 00:00:00 EDT 2006

Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion.

The Journal of biological chemistry

Hiltunen M,Lu A,Thomas AV,Romano DM,Kim M,Jones PB,Xie Z,Kounnas MZ,Wagner SL,Berezovska O,Hyman BT,Tesco G,Bertram L,Tanzi RE

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Western Blot

Fri Oct 27 00:00:00 EDT 2006

Energy inhibition elevates beta-secretase levels and activity and is potentially amyloidogenic in APP transgenic mice: possible early events in Alzheimer's disease pathogenesis.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Velliquette RA,O'Connor T,Vassar R

PA1-757 was used in western blot to investigate the role of impaired energy production in the brain in the AD pathogenesis.

Wed Nov 23 00:00:00 EST 2005

Statins cause intracellular accumulation of amyloid precursor protein, beta-secretase-cleaved fragments, and amyloid beta-peptide via an isoprenoid-dependent mechanism.

The Journal of biological chemistry

Cole SL,Grudzien A,Manhart IO,Kelly BL,Oakley H,Vassar R

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Western Blot

Fri May 13 00:00:00 EDT 2005

Statins cause intracellular accumulation of amyloid precursor protein, beta-secretase-cleaved fragments, and amyloid beta-peptide via an isoprenoid-dependent mechanism.

The Journal of biological chemistry

Cole SL,Grudzien A,Manhart IO,Kelly BL,Oakley H,Vassar R

Published figure using BACE1 polyclonal antibody (Product # PA1-757) in Western Blot

Fri May 13 00:00:00 EDT 2005

BACE is degraded via the lysosomal pathway.

The Journal of biological chemistry

Koh YH,von Arnim CA,Hyman BT,Tanzi RE,Tesco G

PA1-757 was used in western blot to investigate BACE degradation via the lysosomal pathway.

Fri Sep 16 00:00:00 EDT 2005

The metabolism of beta-amyloid converting enzyme and beta-amyloid precursor protein processing.

Biochemical and biophysical research communications

Benjannet S,Cromlish JA,Diallo K,Chrétien M,Seidah NG

PA1-757 was used in immunoprecipitation to investigate BACE metabolism and its influence on beta-amyloid production

Fri Dec 03 00:00:00 EST 2004

Presenilin 1 stabilizes the C-terminal fragment of the amyloid precursor protein independently of gamma-secretase activity.

The Journal of biological chemistry

Pitsi D,Octave JN

PA1-757 was used in western blot to demonstrate the effect of presenilin 1 on Abeta production .

Fri Jun 11 00:00:00 EDT 2004

Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE.

Science (New York, N.Y.)

Vassar R,Bennett BD,Babu-Khan S,Kahn S,Mendiaz EA,Denis P,Teplow DB,Ross S,Amarante P,Loeloff R,Luo Y,Fisher S,Fuller J,Edenson S,Lile J,Jarosinski MA,Biere AL,Curran E,Burgess T,Louis JC,Collins F,Treanor J,Rogers G,Citron M

PA1-757 was used in immunoprecipitation to investigate beta-secretase cleavage of APP protein mediated by BACE

Fri Oct 22 00:00:00 EDT 1999

Bioinformatics

Protein Aliases: APP beta-secretase; asp 2; ASP2; Aspartyl protease 2; BACE 1; BACE-1D; BACE-I-432; Beta secretase; Beta-secretase 1; beta-secretase 1 precursor variant 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; beta-site APP-cleaving enzyme 1; Memapsin; Memapsin-2; Memapsin2; Membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2

Gene Aliases: ASP2; BACE; BACE1; C76936; HSPC104; KIAA1149

UniProt ID: (Human) P56817, (Mouse) P56818

Entrez Gene ID: (Human) 23621, (Mouse) 23821

Molecular Function: protease