Western blot analysis of NIH 3T3 whole cell lysate using PA5-19421, BAF53A primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19421 staining of MEF1 whole cell lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human BAF53A.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:400|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-19421 targets BAF53A in IF, IHC (F), and WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-19421 immunogen is synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human BAF53A.
PA5-19421 detects BAF53A which has a predicted molecular weight of approximately 53 kDa.
This gene encodes a family member of actin-related proteins complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.
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