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Western blot analysis of NIH 3T3 whole cell lysate using PA5-19421, BAF53A primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19421 staining of MEF1 whole cell lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human BAF53A.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:400|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-19421 targets BAF53A in IF, IHC (F), and WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-19421 immunogen is synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human BAF53A.
PA5-19421 detects BAF53A which has a predicted molecular weight of approximately 53 kDa.
This gene encodes a family member of actin-related proteins complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
53 kDa BRG1-associated factor A; actin-like 6; actin-like 6A; actin-like protein 6A; actin-related protein 4; actin-related protein Baf53a; ACTL6, ARPN-BETA, Arp4, BAF53A, INO80K, MGC5382, 53 kDa BRG1-associated factor A; arpNbeta; BAF complex 53 kDa subunit; BAF53; BRG1-associated factor 53A; BRG1/brm-associated factor 53A; hArpN beta; INO80 complex subunit K
2810432C06Rik; ACTL6; ACTL6A; AI851094; ARP4; ARPN-BETA; BAF53; BAF53A; C79802; INO80K; RGD1307747