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BAF53A Antibody (PA5-92438) in IHC (P)
Immunohistochemistry analysis of BAF53A in paraffin-embedded human placenta. Samples were incubated with BAF53A Polyclonal antibody (Product # PA5-92438) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
PA5-92438
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-120 of human ACTL6A (NP_0042921)
Conjugate
Form
Liquid
Concentration
1.33 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806500
Product Specific Information
Immunogen sequence: EDCPKVDFPT AIGMVVERDD GSTLMEIDGD KGKQGGPTYY IDTNALRVPR ENMEAISPLK NGMVEDWDSF QAILDHTYKM HVKSEASLHP V^M
^M
Positive Samples: HT-29, K-562, MCF7, BT-474, Mouse spleen, Mouse testis, Mouse ovary, Mouse thymus, Mouse heart; Cellular Location: Nucleus
Target Information
BAF53A is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 53 kDa BRG1-associated factor A; actin-like 6; actin-like 6A; actin-like 6B; Actin-like protein 6A; actin-related protein 4; Actin-related protein Baf53a; ACTL6B; ArpNbeta; BAF complex 53 kDa subunit; BAF53; BAF53A; BRG1-associated factor 53A; BRG1/brm-associated factor 53A; hArpN alpha; hArpN beta; INO80 complex subunit K; MGC5382; MGC5382 Arp4
Gene Aliases: 2810432C06Rik; ACTL6; ACTL6A; AI851094; ARP4; ARPN-BETA; BAF53; BAF53A; C79802; INO80K; RGD1307747
UniProt ID: (Human) O96019, (Mouse) Q9Z2N8
Entrez Gene ID: (Human) 86, (Mouse) 56456, (Rat) 361925
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
chromatin binding
transcription coactivator activity
protein binding
nucleosomal DNA binding
molecular_function
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
neural retina development
DNA repair
DNA recombination
chromatin remodeling
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
signal transduction
spinal cord development
regulation of growth
ATP-dependent chromatin remodeling
histone H4 acetylation
histone H2A acetylation
regulation of mitophagy
positive regulation of protein targeting to mitochondrion
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
nervous system development
chromatin modification
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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