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Invitrogen
BAF53B Polyclonal Antibody
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FIGURE: 1 / 3
BAF53B Antibody (PA5-34896) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of BAF53B was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: BAF53B Polyclonal Antibody (Product # PA5-34896) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Product Details
PA5-34896
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human BAF53B. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.62 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: SK-N-SH.
Predicted reactivity: Mouse (99%), Rat (99%), Rhesus Monkey (100%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Belongs to the chromatin remodeling brain-specific BAF (bBAF) complex, as such plays a role in remodeling mononucleosomes in an ATP-dependent fashion. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex) and is required for postmitotic neural development and dendritic outgrowth. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. ACTL6B/BAF53B is not essential for assembly of the nBAF complex but is required for targeting the complex and CREST to the promoter of genes essential for dendritic growth.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 53 kDa BRG1-associated factor B; actin-like 6; Actin-like protein 6B; actin-like protein 6B {ECO:0000250|UniProtKB:O94805}; Actin-related protein Baf53b; actl6b {ECO:0000250|UniProtKB:O94805}; ArpNalpha; BAF53B; BRG1-associated factor 53B; hArpN alpha; unnamed protein product
Gene Aliases: ACTL6; ACTL6B; arpNalpha; BAF53B; DEE76; EIEE76; IDDSSAD; SMARCN2
UniProt ID: (Human) O94805, (Rat) P86173
Entrez Gene ID: (Human) 51412, (Rat) 288563
chromatin organization
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
cytoskeleton organization
nervous system development
positive regulation of cell proliferation
dendrite development
regulation of mitotic metaphase/anaphase transition
neuron maturation
positive regulation of T cell differentiation
negative regulation of cell differentiation
positive regulation of cell differentiation
positive regulation of myoblast differentiation
positive regulation of transcription, DNA-templated
regulation of G0 to G1 transition
positive regulation of stem cell population maintenance
regulation of G1/S transition of mitotic cell cycle
positive regulation of double-strand break repair
regulation of nucleotide-excision repair
transcription, DNA-templated
regulation of transcription, DNA-templated
spinal cord development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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