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Invitrogen
BAG2 Polyclonal Antibody
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FIGURE: 1 / 9
BAG2 Antibody (PA5-78853) in ICC/IF
Immunocytochemistry analysis of BAG2 using anti-BAG2 antibody (Product # PA5-78853). BAG2 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5 μg/mL rabbit anti-BAG2 antibody (Product # PA5-78853) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstai... View More
Product Details
PA5-78853
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human BAG2 recombinant protein (Position: M1-N211).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Jurkat whole cell, human Hela whole cell, human HepG2 whole cell, human K562 whole cell. ICC/IF: A431 cell. Flow: THP-1 cell.
Target Information
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BAG family molecular chaperone regulator 2; BAG-2; BAG2; Bcl-2-associated athanogene 2; BCL2 associated athanogene 2; dJ417I1.2 (BAG-family molecular chaperone regulator 2); KIAA0576; MGC149462; RP3-496N17.2; unnamed protein product
Gene Aliases: BAG-2; BAG2; dJ417I1.2
UniProt ID: (Human) O95816
Entrez Gene ID: (Human) 9532
protein folding
positive regulation of protein processing
protein metabolic process
negative regulation of protein ubiquitination
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of proteolysis
protein stabilization
positive regulation of proteasomal protein catabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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