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Rockland
BAG4 Polyclonal Antibody
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FIGURE: 1 / 3
BAG4 Antibody (200-401-ES1) in ICC/IF
Immunocytochemistry of BAG4 antibody. Cells: HeLa cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: SODD antibody at 5 µg/mL for 1 hr at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: SODD is cytoplasmic. Staining: BAG4 as precipitated brown signal with hematoxylin blue nuclear counterstain.
Product Details
200-401-ES1
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-BAG4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to amino acids near the N-terminus of human BAG4.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Ion-exchange chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. This protein was found to be associated with the death domain of tumor necrosis factor receptor type 1 (TNF-R1) and death receptor-3 (DR3), and thereby negatively regulates downstream cell death signaling. The regulatory role of this protein in cell death was demonstrated in epithelial cells which undergo apoptosis while integrin mediated matrix contacts are lost.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BAG family molecular chaperone regulator 4; BAG-4; BAG-family molecular chaperone regulator-4; Bcl-2-associated athanogene 4; BCL2-associated athanogene 4; Silencer of death domains
Gene Aliases: 2410112I15Rik; BAG-4; BAG4; SODD
UniProt ID: (Human) O95429, (Mouse) Q8CI61
Entrez Gene ID: (Human) 9530, (Mouse) 67384, (Rat) 361167
apoptotic process
positive regulation of fibroblast migration
positive regulation of actin filament polymerization
positive regulation of peptidyl-serine phosphorylation
regulation of apoptotic process
positive regulation of cell adhesion
protein heterooligomerization
positive regulation of stress fiber assembly
positive regulation of protein kinase B signaling
cellular response to tumor necrosis factor
cellular response to epidermal growth factor stimulus
protein localization to plasma membrane
negative regulation of mRNA modification
ruffle assembly
negative regulation of protein targeting to mitochondrion
negative regulation of phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity
protein folding
tumor necrosis factor-mediated signaling pathway
negative regulation of apoptotic process
regulation of cellular response to heat
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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