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Invitrogen
BAG6 Polyclonal Antibody
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FIGURE: 1 / 6
BAG6 Antibody (PA5-95614) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of BAG6 in PC3 cell using BAG6 Polyclonal Antibody (Product # PA5-95614). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL and mouse anti-Beta Tubulin antibody overnight at 4°C. DyLight488 conjugated goat anti-rabbit IgG and DyLight 550 conjugated goat anti-mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. V... View More
Product Details
PA5-95614
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human BAT3/BAG6 recombinant protein (D15-A88).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human U87 whole cell, human SH-SY5Y whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell, human Hela whole cell, human THP-1 whole cell, human U87 whole cell, human SH-SY5Y whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell. IHC: human lung cancer tissue, human ovarian serous adenocarcinoma tissue. ICC/IF: PC3 cell. Flow: SH-SY5Y cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene was first characterized as part of a cluster of genes located within the human major histocompatibility complex class III region. This gene encodes a nuclear protein that is cleaved by caspase 3 and is implicated in the control of apoptosis. In addition, the protein forms a complex with E1A binding protein p300 and is required for the acetylation of p53 in response to DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BAG 6; BAG family molecular chaperone regulator 6; BAG-6; BAT 3; BCL2-associated athanogene 6; DAQB-195H10.3; HLA-B associated transcript 3; HLA-B associated transcript-3; HLA-B-associated transcript 3; Large proline-rich protein BAG6; large proline-rich protein BAT3; Protein G3; Protein Scythe; scythe
Gene Aliases: 2410045D21Rik; AA408914; BAG-6; BAG6; BAT3; D17H6S52E; D6S52E; G3; Scythe
UniProt ID: (Human) Q5SQ35, (Rat) Q6MG49, (Mouse) Q9Z1R2
Entrez Gene ID: (Human) 7917, (Rat) 94342, (Mouse) 224727
kidney development
immune system process
ubiquitin-dependent protein catabolic process
transport
synaptonemal complex assembly
spermatogenesis
brain development
embryo development
covalent chromatin modification
internal peptidyl-lysine acetylation
cell differentiation
lung development
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
regulation of cell proliferation
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
negative regulation of apoptotic process
negative regulation of proteolysis
protein stabilization
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
tail-anchored membrane protein insertion into ER membrane
positive regulation of ERAD pathway
maintenance of unfolded protein involved in ERAD pathway
protein localization to cytosolic proteasome complex involved in ERAD pathway
chromatin modification
regulation of apoptotic process
antigen processing and presentation of peptide antigen via MHC class I
apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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