|Immunohistochemistry (Paraffin) (IHC (P))||1:300|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from N-terminus of human Bak|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody is predicted to react with porcine and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is tonsil tissue.
Apoptosis or programmed cell death is a physiological cellular process characterized by cell shrinkage, membrane blebbing, DNA fragmentation, and release of Cytochrome C from the mitochondria. It is utilized by the organism to get rid of unwanted cells, which is critical for normal development and homeostasis of an organism. Disregulation of normal apoptosis process have been implicated in a variety of diseases, including cancer, autoimmune diseases, viral infections, etc. Programmed cell death occurs through complex cascades of cell signaling in which Bcl2 family members, among others, play an important role.The Bcl2 family of proteins regulate apoptosis as well as execute death signals at the mitochondrion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: apoptosis regulator BAK; BAK; BAK-LIKE; bcl-2 homologous antagonist/killer; bcl-2-like protein 7; bcl2-L-7; BCL2-like 7 protein; BCL2L7; CDN1; MGC117255; MGC3887; pro-apoptotic protein BAK
Gene Aliases: BAK; BAK-LIKE; BCL2L7; CDN1
UniProt ID: (Human) Q16611
Entrez Gene ID: (Human) 578
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