|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 14 amino acid peptide near the center of human BANP.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||10 µg/ml|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is mouse kidney tissue lysate.
PA5-21030 can be used with blocking peptide PEP-1144.
BANP was initially identified as a binding protein to BTG3 in a yeast two-hybrid screen. BANP acts as a tumor suppressor by stabilizing p53 expression and leading to cell cycle arrest. p53 in turn binds to upstream elements of the BANP promoter, thereby forming a feedback loop. BANP is down-regulated in advanced stages of human breast cancer, and its overexpression in breast cancer cell lines inhibits their ability to metastasize by modulating TGF-beta signaling. Furthermore, BANP can modulate NF-kappa-B transactivation and can inhibit tumorigenesis by regulating NF-kappa-B target genes. Recent experiments have shown that BANP can also repress HIV-1 LTR mediated transcription by tethering the LTR matrix attachment region to nuclear matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.