Proteintech

BBS3 Polyclonal Antibody

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BBS3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MDCK cells using 12676-1-AP (BBS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

BBS3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney tissue slide using 12676-1-AP ( BBS3 Antibody) at dilution of 1:200 (under 40x lens).

BBS3 Antibody in Western Blot (WB) — Human testis tissue were subjected to SDS PAGE followed by western blot with 12676-1-AP (BBS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

BBS3 Antibody in Immunoprecipitation (IP) — IP result of anti-BBS3 (IP: 12676-1-AP, 3 µg; Detection: 12676-1-AP 1:600) with rat brain tissue lysate 5200 µg.

Product Details

12676-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	BBS3 Fusion Protein Ag3363 (1-186 aa encoded by BC024239) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MGLLDRLSV LLGLKKKEVH VLCLGLDNSG KTTIINKLKP SNAQSQNILP TIGFSIEKFK SSSLSFTVFD MSGQGRYRNL WEHYYKEGQA IIFVIDSSDR LRMVVAKEEL DTLLNHPDIK HRRIPILFFA NKMDLRDAVT SVKVSQLLCL ENIKDKPWHI CASDAIKGEG LQEGVDWLQD QIQTVKT (1-186 aa encoded by BC024239)

Target Information

The protein encoded by this gene belongs to the ARF-like (ADP ribosylation factor-like) sub-family of the ARF family of GTP-binding proteins which are involved in regulation of intracellular traffic. Mutations in this gene are associated with Bardet-Biedl syndrome (BBS). Alternative splicing results in multiple transcript variants of this gene. A vision-specific transcript encoding a different protein has been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.

PLoS genetics

Hsu Y,Garrison JE,Kim G,Schmitz AR,Searby CC,Zhang Q,Datta P,Nishimura DY,Seo S,Sheffield VC

Sun Oct 01 00:00:00 EDT 2017

Bardet-Biedl syndrome 3 regulates the development of cranial base midline structures.

Bone

Kawasaki M,Izu Y,Hayata T,Ideno H,Nifuji A,Sheffield VC,Ezura Y,Noda M

Tue Aug 01 00:00:00 EDT 2017

Basal body proteins regulate Notch signaling through endosomal trafficking.

Journal of cell science

Leitch CC,Lodh S,Prieto-Echagüe V,Badano JL,Zaghloul NA

Sun Jun 01 00:00:00 EDT 2014

ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.

Proceedings of the National Academy of Sciences of the United States of America

Humbert MC,Weihbrecht K,Searby CC,Li Y,Pope RM,Sheffield VC,Seo S

Tue Nov 27 00:00:00 EST 2012

Bioinformatics

Protein Aliases: ADP-ribosylation factor-like protein 6; ADP-ribosylation-like 6; ADP-ribosylation-like factor homolog ARL6; Bardet-Biedl syndrome 3 protein

Gene Aliases: 1110018H24Rik; 2210411E14Rik; ARL6; BBS3; RP55

UniProt ID: (Human) Q9H0F7, (Mouse) O88848

Entrez Gene ID: (Human) 84100, (Rat) 363760, (Mouse) 56297

Molecular Function: G-protein