Proteintech

BBS4 Polyclonal Antibody

View all (12) BBS4 antibodies

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BBS4 Antibody in Immunocytochemistry (ICC/IF) — IF result (cytoplasm and the length of cilia cilia stain) of anti-BBS4 (12766-1-AP; 1:50) with serum-starved hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim.

BBS4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 12766-1-AP (BBS4 antibody) at dilution of 1:50 (under 40x lens).

BBS4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 12766-1-AP (BBS4 antibody) at dilution of 1:50 (under 10x lens).

BBS4 Antibody in Western Blot (WB) — Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12766-1-AP (BBS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

BBS4 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 12766-1-AP (BBS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

BBS4 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 12766-1-AP (BBS4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

BBS4 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 12766-1-AP (BBS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

12766-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 4 publications 4 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	BBS4 Fusion Protein Ag3430 (1-351 aa encoded by BC027624) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.14 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MAEERVATR TQFPVSTESQ KPRQKKAPEF PILEKQNWLI HLHYIRKDYE ACKAVIKEQL QETQGLCEYA IYVQALIFRL EGNIQESLEL FQTCAVLSPQ SADNLKQVAR SLFLLGKHKA AIEVYNEAAK LNQKDWEISH NLGVCYIYLK QFNKAQDQLH NALNLNRHDL TYIMLGKIHL LEGDLDKAIE VYKKAVEFSP ENTELLTTLG LLYLQLGIYQ KAFEHLGNAL TYDPTNYKAI LAAGSMMQTH GDFDVALTKY RVVACAVPES PPLWNNIGMC FFGKKKYVAA ISCLKRANYL APFDWKILYN LGLVHLTMQQ YASAFHFLSA AINFQPKMGE LYMLLAVALT NL (1-351 aa encoded by BC027624)

Target Information

BBS4 contains tetratricopeptide repeats (TPR), similar to O-linked N-acetyglucosamine transferase. Mutations in the gene encoding this protein have been observed in patients with Bardet-Biedl syndrome type 4. BBS4 may play a role in pigmentary retinopathy, obesity, polydactyly, renal malformation and mental retardation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts.

Autophagy

Struchtrup A,Wiegering A,Stork B,Rüther U,Gerhardt C

Thu Mar 07 00:00:00 EST 2019

Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets.

Nature communications

Krastev DB,Pettitt SJ,Campbell J,Song F,Tanos BE,Stoynov SS,Ashworth A,Lord CJ

Tue May 22 00:00:00 EDT 2018

BBS4 regulates the expression and secretion of FSTL1, a protein that participates in ciliogenesis and the differentiation of 3T3-L1.

Scientific reports

Prieto-Echagüe V,Lodh S,Colman L,Bobba N,Santos L,Katsanis N,Escande C,Zaghloul NA,Badano JL

Tue Aug 29 00:00:00 EDT 2017

BBS4 regulates the expression and secretion of FSTL1, a protein that participates in ciliogenesis and the differentiation of 3T3-L1.

Scientific reports

Prieto-Echagüe V,Lodh S,Colman L,Bobba N,Santos L,Katsanis N,Escande C,Zaghloul NA,Badano JL

Tue Aug 29 00:00:00 EDT 2017

DAZ-interacting Protein 1 (Dzip1) Phosphorylation by Polo-like Kinase 1 (Plk1) Regulates the Centriolar Satellite Localization of the BBSome Protein during the Cell Cycle.

The Journal of biological chemistry

Zhang B,Wang G,Xu X,Yang S,Zhuang T,Wang G,Ren H,Cheng SY,Jiang Q,Zhang C

Fri Jan 27 00:00:00 EST 2017

DAZ-interacting Protein 1 (Dzip1) Phosphorylation by Polo-like Kinase 1 (Plk1) Regulates the Centriolar Satellite Localization of the BBSome Protein during the Cell Cycle.

The Journal of biological chemistry

Zhang B,Wang G,Xu X,Yang S,Zhuang T,Wang G,Ren H,Cheng SY,Jiang Q,Zhang C

Fri Jan 27 00:00:00 EST 2017

BBS4 and BBS5 show functional redundancy in the BBSome to regulate the degradative sorting of ciliary sensory receptors.

Scientific reports

Xu Q,Zhang Y,Wei Q,Huang Y,Li Y,Ling K,Hu J

Tue Jul 07 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Bardet-Biedl syndrome 4 homolog; Bardet-Biedl syndrome 4 protein; Bardet-Biedl syndrome 4 protein homolog

Gene Aliases: AW537059; AW742241; BBS4; D9Ertd464e

UniProt ID: (Human) Q96RK4, (Mouse) Q8C1Z7

Entrez Gene ID: (Human) 585, (Mouse) 102774, (Rat) 300754

microtubule cytoskeleton organization mitotic cytokinesis neural tube closure retina homeostasis negative regulation of systemic arterial blood pressure transport spermatid development sensory perception of smell protein localization metabolic process negative regulation of gene expression protein transport dendrite development regulation of lipid metabolic process ventricular system development striatum development hippocampus development cerebral cortex development cell projection organization adult behavior negative regulation of actin filament polymerization regulation of cytokinesis leptin-mediated signaling pathway protein localization to organelle negative regulation of GTPase activity microtubule anchoring at centrosome nonmotile primary cilium assembly social behavior photoreceptor cell outer segment organization negative regulation of appetite by leptin-mediated signaling pathway positive regulation of multicellular organism growth cilium assembly response to leptin photoreceptor cell maintenance positive regulation of cilium assembly retinal rod cell development brain morphogenesis centrosome organization maintenance of protein location in nucleus regulation of stress fiber assembly cilium morphogenesis regulation of cilium beat frequency involved in ciliary motility face development fat pad development protein localization to cilium protein localization to centrosome regulation of nonmotile primary cilium assembly protein localization to photoreceptor outer segment neuron migration heart looping visual perception melanosome transport fat cell differentiation intracellular transport sensory processing biological_process

Process(es)

RNA polymerase II repressing transcription factor binding microtubule motor activity protein binding dynactin binding alpha-tubulin binding beta-tubulin binding molecular_function