Invitrogen

BBS4 Polyclonal Antibody

View all (6) BBS4 antibodies

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BBS4 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of BBS4 in human duodenum tissue shows strong cytoplasmic and membranous positivity in glandular cells. Samples were probed using a BBS4 Polyclonal Antibody (Product # PA5-58783).

BBS4 Antibody in Western Blot (WB) — Western blot analysis of BBS4 in control (vector only transfected HEK293T lysate) and BBS4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a BBS4 Polyclonal Antibody (Product # PA5-58783).

BBS4 Antibody in Western Blot (WB) — Western blot analysis of BBS4 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4. Samples were probed using a BBS4 Polyclonal Antibody (Product # PA5-58783).

Product Details

Tested Applications

Dilution

Immunohistochemistry (IHC)

1:200-1:500

Western Blot (WB)

0.4 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human BBS4

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2638489

Product Specific Information

Immunogen sequence: TQFPVSTESQ KPRQKKAPEF PILEKQNWLI HLHYIRKDYE ACKAVIKEQL QETQGLCEYA IYVQALIFRL EGNIQESL

Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 91%.

Target Information

BBS4 contains tetratricopeptide repeats (TPR), similar to O-linked N-acetyglucosamine transferase. Mutations in the gene encoding this protein have been observed in patients with Bardet-Biedl syndrome type 4. BBS4 may play a role in pigmentary retinopathy, obesity, polydactyly, renal malformation and mental retardation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Bardet-Biedl syndrome 4 protein; BBS4

Gene Aliases: BBS4

UniProt ID: (Human) Q96RK4

Entrez Gene ID: (Human) 585

Molecular Function: glycosyltransferase transferase

microtubule cytoskeleton organization mitotic cytokinesis neuron migration neural tube closure retina homeostasis heart looping negative regulation of systemic arterial blood pressure spermatid development visual perception sensory perception of smell negative regulation of gene expression protein transport dendrite development regulation of lipid metabolic process ventricular system development striatum development hippocampus development cerebral cortex development adult behavior negative regulation of actin filament polymerization melanosome transport regulation of cytokinesis protein localization to organelle negative regulation of GTPase activity microtubule anchoring at centrosome nonmotile primary cilium assembly social behavior photoreceptor cell outer segment organization negative regulation of appetite by leptin-mediated signaling pathway positive regulation of multicellular organism growth cilium assembly fat cell differentiation photoreceptor cell maintenance positive regulation of cilium assembly retinal rod cell development intracellular transport brain morphogenesis sensory processing centrosome organization maintenance of protein location in nucleus regulation of stress fiber assembly cilium morphogenesis regulation of cilium beat frequency involved in ciliary motility face development fat pad development protein localization to cilium protein localization to centrosome regulation of nonmotile primary cilium assembly protein localization to photoreceptor outer segment

Process(es)

RNA polymerase II repressing transcription factor binding microtubule motor activity protein binding dynactin binding alpha-tubulin binding beta-tubulin binding