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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
0.5-1 µg/mL | - |
Immunohistochemistry (IHC) |
5 µg/mL | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
A 14 amino acid peptide near the carboxy terminus of human BCAS2. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS |
Contains |
0.02% sodium azide |
Storage conditions |
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C |
RRID |
AB_11153389 |
A suggested positive control is MCF7 cell lysate.
PA5-20905 can be used with blocking peptide PEP-1019.
cDNA encoding BCAS2 has been isolated. The deduced 225-amino acid protein contains a central potential N-glycosylation site and multiple N-terminal potential phosphorylation sites. Northern and Southern blot analyses demonstrates expression of a 1.5-kb transcript at various levels in multiple breast cancer cell lines, with greatest amplification in BT-20 and MCF-7 cells. BCAS2 is ubiquitously expressed in normal tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Breast carcinoma-amplified sequence 2; Breast carcinoma-amplified sequence 2 homolog; DNA amplified in mammary carcinoma 1 protein; Pre-mRNA-splicing factor SPF27; spliceosome associated protein, amplified in breast cancer; Spliceosome-associated protein SPF 27
Gene Aliases: 6430539P16Rik; AI132645; BCAS2; C76366; C80030; DAM1; Snt309; SPF27
UniProt ID: (Human) O75934, (Mouse) Q9D287
Entrez Gene ID: (Human) 10286, (Rat) 295334, (Mouse) 68183
Molecular Function:
RNA splicing factor
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